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Literature summary for 1.14.13.148 extracted from

  • Haining, R.; Hunter, A.; Sadeque, A.; Philpot, R.; Rettie, A.
    Baculovirus-mediated expression and purification of human FMO3: Catalytic, immunochemical, and structural characterization (1997), Drug Metab. Dispos., 25, 790-797.
    View publication on PubMed
Search for more literature for 1.14.13.148

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Trichoplusia ni cells and in Escherichia coli strain XL-1 Blue Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Homo sapiens
-
-

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein N-linked glycosylation at Asn61 and O-linked glycosylation at Thr29, Thr249, and Thr381. Posttranslational modification of human FMO3 by insect cells is limited to cleavage at the N-terminal methionine Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
cholate solubilization and sequential column chromatography on octyl-Sepharose, DEAE-Sepharose, and hydroxyapatite Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
liver FMO3 is quantitatively a major human liver monooxygenase Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.049
-
 crude enzyme from membranes, using methyl 4-tolyl sulfide as substrate, in 25 mM potassium diphosphate (pH 8.5), at 37°C Homo sapiens
0.201
-
 after purification, using methyl 4-tolyl sulfide as substrate, in 25 mM potassium diphosphate (pH 8.5), at 37°C Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methyl 4-tolyl sulfide + NADPH + H+ + O2
-
 Homo sapiens methyl 4-tolyl sulfoxide + NADP+ + H2O
-
 ?

Synonyms

Synonyms Comment Organism
FMO3
-
 Homo sapiens

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Homo sapiens
NADPH required for activity Homo sapiens