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Literature summary for 1.14.13.146 extracted from

  • Jennewein, S.; Rithner, C.D.; Williams, R.M.; Croteau, R.
    Taxoid metabolism: Taxoid 14beta-hydroxylase is a cytochrome P450-dependent monooxygenase (2003), Arch. Biochem. Biophys., 413, 262-270.
    View publication on PubMed

Application

Application Comment Organism
medicine manipulation of the hydroxylase gene can permit redirection of the pathway to increase flux toward taxol and can allow the preparation of 13alpha,14beta-hydroxy taxoids as new therapeutic agents Taxus cuspidata

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Saccharomyces cerevisiae WAT11 cells Taxus cuspidata

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.033
-
5alpha-acetoxytaxadiene in Tris-HCl pH 7.5, temperature not specified in the publication Taxus cuspidata
0.055
-
5alpha-acetoxy-10beta-hydroxytaxadiene in Tris-HCl pH 7.5, temperature not specified in the publication Taxus cuspidata

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
Taxus cuspidata
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57146
-
x * 57146, calculated from amino acid sequence Taxus cuspidata

Organism

Organism UniProt Comment Textmining
Taxus cuspidata Q84KI1
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5alpha-acetoxy-10beta-hydroxytaxadiene + O2 + NADPH + H+
-
Taxus cuspidata 5alpha-acetoxy-10beta,14beta-dihydroxytaxadiene + NADP+ + H2O
-
?
5alpha-acetoxytaxadiene + O2 + NADPH + H+
-
Taxus cuspidata ? + NADP+ + H2O
-
?

Subunits

Subunits Comment Organism
? x * 57146, calculated from amino acid sequence Taxus cuspidata

Synonyms

Synonyms Comment Organism
cytochrome P450 taxoid 14beta-hydroxylase
-
Taxus cuspidata
taxoid 14beta-hydroxylase
-
Taxus cuspidata

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Taxus cuspidata

Cofactor

Cofactor Comment Organism Structure
cytochrome P450 dependent on Taxus cuspidata