Application | Comment | Organism |
---|---|---|
medicine | manipulation of the hydroxylase gene can permit redirection of the pathway to increase flux toward taxol and can allow the preparation of 13alpha,14beta-hydroxy taxoids as new therapeutic agents | Taxus cuspidata |
Cloned (Comment) | Organism |
---|---|
expressed in Saccharomyces cerevisiae WAT11 cells | Taxus cuspidata |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.033 | - |
5alpha-acetoxytaxadiene | in Tris-HCl pH 7.5, temperature not specified in the publication | Taxus cuspidata | |
0.055 | - |
5alpha-acetoxy-10beta-hydroxytaxadiene | in Tris-HCl pH 7.5, temperature not specified in the publication | Taxus cuspidata |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Taxus cuspidata | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
57146 | - |
x * 57146, calculated from amino acid sequence | Taxus cuspidata |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Taxus cuspidata | Q84KI1 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5alpha-acetoxy-10beta-hydroxytaxadiene + O2 + NADPH + H+ | - |
Taxus cuspidata | 5alpha-acetoxy-10beta,14beta-dihydroxytaxadiene + NADP+ + H2O | - |
? | |
5alpha-acetoxytaxadiene + O2 + NADPH + H+ | - |
Taxus cuspidata | ? + NADP+ + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 57146, calculated from amino acid sequence | Taxus cuspidata |
Synonyms | Comment | Organism |
---|---|---|
cytochrome P450 taxoid 14beta-hydroxylase | - |
Taxus cuspidata |
taxoid 14beta-hydroxylase | - |
Taxus cuspidata |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Taxus cuspidata |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome P450 | dependent on | Taxus cuspidata |