Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Rhodococcus erythropolis | |
HgCl2 | 1 mM, 85% inhibition | Rhodococcus erythropolis | |
additional information | no inhibition with 1 mM iodoacetate, iodoacetamide, EDTA, 2,2'-dipyridyl and DTT | Rhodococcus erythropolis | |
p-chloromercuriobenzoate | 0.1 mM, 14% inhibition | Rhodococcus erythropolis | |
phenylhydrazine | 1 mM, 10% inhibition | Rhodococcus erythropolis | |
SDS | 1 mM, 97% inhibition | Rhodococcus erythropolis | |
ZnCl2 | 1 mM 33% inhibition | Rhodococcus erythropolis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.038 | - |
NADPH | - |
Rhodococcus erythropolis | |
0.12 | - |
(1R,4S)-menthone | 30°C and glyine/NaOH buffer, pH 9.5 | Rhodococcus erythropolis | |
0.13 | - |
(1S,4R)-1-hydroxy-2-oxolimonene | 30°C and glyine/NaOH buffer, pH 9.5 | Rhodococcus erythropolis | |
0.13 | - |
(4R)-dihydrocarvone | 30°C and glyine/NaOH buffer, pH 9.5 | Rhodococcus erythropolis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
57000 | - |
gel filtration | Rhodococcus erythropolis |
60000 | - |
1 * 60000, SDS-PAGE | Rhodococcus erythropolis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Rhodococcus erythropolis | MMKMO is involved in the conversion of the monocyclic monoterpene ketone intermediates formed in the degradation pathways of all stereoisomers of three different monocyclic monoterpenes, i.e. limonene, (dihydro)carveol and menthol. MMKMO converts all enantiomers of the natural substrates with almost equal efficiency | ? | - |
? | |
additional information | Rhodococcus erythropolis DCL14 | MMKMO is involved in the conversion of the monocyclic monoterpene ketone intermediates formed in the degradation pathways of all stereoisomers of three different monocyclic monoterpenes, i.e. limonene, (dihydro)carveol and menthol. MMKMO converts all enantiomers of the natural substrates with almost equal efficiency | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus erythropolis | - |
- |
- |
Rhodococcus erythropolis DCL14 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rhodococcus erythropolis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(1R)-pulegone + NADPH + H+ + O2 | 31% of the activity with (1R,4R)-dihydrocarvone | Rhodococcus erythropolis | ? | - |
? | |
(1R)-pulegone + NADPH + H+ + O2 | 31% of the activity with (1R,4R)-dihydrocarvone | Rhodococcus erythropolis DCL14 | ? | - |
? | |
(1R,4R)-dihydrocarvone + NADPH + H+ + O2 | - |
Rhodococcus erythropolis | (4R,7R)-4-isopropenyl-7-methyl-2-oxo-oxepanone + NADP+ + H2O | - |
? | |
(1R,4R)-dihydrocarvone + NADPH + H+ + O2 | - |
Rhodococcus erythropolis DCL14 | (4R,7R)-4-isopropenyl-7-methyl-2-oxo-oxepanone + NADP+ + H2O | - |
? | |
(1R,4S)-1-hydroxy-2-oxolimonene + NADPH + H+ + O2 | 3-isopropenyl-6-oxoheptanoate is the spontaneous rearrangement product of the lactone formed by MMKMO. 133% of the activity with (1R,4R)-dihydrocarvone | Rhodococcus erythropolis | 3-isopropenyl-6-oxoheptanoate + NADP+ + H2O | - |
? | |
(1R,4S)-1-hydroxy-2-oxolimonene + NADPH + H+ + O2 | 3-isopropenyl-6-oxoheptanoate is the spontaneous rearrangement product of the lactone formed by MMKMO. 133% of the activity with (1R,4R)-dihydrocarvone | Rhodococcus erythropolis DCL14 | 3-isopropenyl-6-oxoheptanoate + NADP+ + H2O | - |
? | |
(1R,4S)-menthone + NADPH + H+ + O2 | 76% of the activity with (1R,4R)-dihydrocarvone | Rhodococcus erythropolis | (4R,7S)-7-isopropyl-4-methyl-2-oxo-oxepanone + NADP+ + H2O | - |
? | |
(1S)-pulegone + NADPH + H+ + O2 | 51% of the activity with (1R,4R)-dihydrocarvone | Rhodococcus erythropolis | ? | - |
? | |
(1S,4R)-1-hydroxy-2-oxolimonene + NADPH + H+ + O2 | 3-isopropenyl-6-oxoheptanoate is the spontaneous rearrangement product of the lactone formed by MMKMO. 88% of the activity with (1R,4R)-dihydrocarvone | Rhodococcus erythropolis | 3-isopropenyl-6-oxoheptanoate + NADP+ + H2O | - |
? | |
(1S,4R)-dihydrocarvone + NADPH + H+ + O2 | - |
Rhodococcus erythropolis | ? | - |
? | |
(1S,4R)-menthone + NADPH + H+ + O2 | 82% of the activity with (1R,4R)-dihydrocarvone | Rhodococcus erythropolis | (4S,7R)-7-isopropyl-4-methyl-2-oxo-oxepanone + NADP+ + H2O | - |
? | |
(1S,4R)iso-dihydrocarvone + NADPH + H+ + O2 | - |
Rhodococcus erythropolis | (3S,4R)-6-isopropenyl-3-methyl-2-oxo-oxepanone + NADP+ + H2O | - |
? | |
(4S)-carvone + NADPH + H+ + O2 | 14% of the activity with (1R,4R)-dihydrocarvone | Rhodococcus erythropolis | ? | - |
? | |
1,2-cyclohexanedione + NADPH + H+ + O2 | 95% of the activity with (1R,4R)-dihydrocarvone | Rhodococcus erythropolis | ? | - |
? | |
1,4-cyclohexanedione + NADPH + H+ + O2 | 92% of the activity with (1R,4R)-dihydrocarvone | Rhodococcus erythropolis | ? | - |
? | |
2,2-dimethylcyclohexanone + NADPH + H+ + O2 | 161% of the activity with (1R,4R)-dihydrocarvone | Rhodococcus erythropolis | ? | - |
? | |
2,6-dimethylcyclohexanone + NADPH + H+ + O2 | 108% of the activity with (1R,4R)-dihydrocarvone | Rhodococcus erythropolis | ? | - |
? | |
2-allylcyclohexanone + NADPH + H+ + O2 | 145% of the activity with (1R,4R)-dihydrocarvone | Rhodococcus erythropolis | ? | - |
? | |
2-chlorocyclohexanone + NADPH + H+ + O2 | 112% of the activity with (1R,4R)-dihydrocarvone | Rhodococcus erythropolis | ? | - |
? | |
2-methoxycyclohexanone + NADPH + H+ + O2 | 168% of the activity with (1R,4R)-dihydrocarvone | Rhodococcus erythropolis | ? | - |
? | |
2-methylcyclohexanone + NADPH + H+ + O2 | 155% of the activity with (1R,4R)-dihydrocarvone | Rhodococcus erythropolis | ? | - |
? | |
2-phenylcyclohexanone + NADPH + O2 | 148% of the activity with (1R,4R)-dihydrocarvone | Rhodococcus erythropolis | ? | - |
? | |
3-methylcyclohexanone + NADPH + H+ + O2 | 151% of the activity with (1R,4R)-dihydrocarvone | Rhodococcus erythropolis | ? | - |
? | |
4-methylcyclohexanone + NADPH + H+ + O2 | 40% of the activity with (1R,4R)-dihydrocarvone | Rhodococcus erythropolis | ? | - |
? | |
8-mercaptomenthone + NADPH + H+ + O2 | 14% of the activity with (1R,4R)-dihydrocarvone | Rhodococcus erythropolis | ? | - |
? | |
cycloheptanone + NADPH + H+ + O2 | 10% of the activity with (1R,4R)-dihydrocarvone | Rhodococcus erythropolis | ? | - |
? | |
cyclohexanone + NADPH + H+ + O2 | 151% of the activity with (1R,4R)-dihydrocarvone | Rhodococcus erythropolis | ? | - |
? | |
cyclopentanone + NADPH + H+ + O2 | 12% of the activity with (1R,4R)-dihydrocarvone | Rhodococcus erythropolis | ? | - |
? | |
additional information | MMKMO is involved in the conversion of the monocyclic monoterpene ketone intermediates formed in the degradation pathways of all stereoisomers of three different monocyclic monoterpenes, i.e. limonene, (dihydro)carveol and menthol. MMKMO converts all enantiomers of the natural substrates with almost equal efficiency | Rhodococcus erythropolis | ? | - |
? | |
additional information | MMKMO converts all enantiomers of the natural substrates with almost equal efficiency. No activity with NADH | Rhodococcus erythropolis | ? | - |
? | |
additional information | MMKMO is involved in the conversion of the monocyclic monoterpene ketone intermediates formed in the degradation pathways of all stereoisomers of three different monocyclic monoterpenes, i.e. limonene, (dihydro)carveol and menthol. MMKMO converts all enantiomers of the natural substrates with almost equal efficiency | Rhodococcus erythropolis DCL14 | ? | - |
? | |
additional information | MMKMO converts all enantiomers of the natural substrates with almost equal efficiency. No activity with NADH | Rhodococcus erythropolis DCL14 | ? | - |
? | |
norcamphor + NADPH + H+ + O2 | 145% of the activity with (1R,4R)-dihydrocarvone | Rhodococcus erythropolis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 60000, SDS-PAGE | Rhodococcus erythropolis |
Synonyms | Comment | Organism |
---|---|---|
Baeyer-Villiger mono-oxygenase | - |
Rhodococcus erythropolis |
BVMO | - |
Rhodococcus erythropolis |
MMKMO | - |
Rhodococcus erythropolis |
monocyclic monoterpene ketone mono-oxygenase | - |
Rhodococcus erythropolis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
36 | - |
- |
Rhodococcus erythropolis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
inactivation above | Rhodococcus erythropolis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.6 | - |
(1R,4S)-menthone | 30°C and glyine/NaOH buffer, pH 9.5 | Rhodococcus erythropolis | |
3.9 | - |
(1S,4R)-1-hydroxy-2-oxolimonene | 30°C and glyine/NaOH buffer, pH 9.5 | Rhodococcus erythropolis | |
3.9 | - |
(4R)-dihydrocarvone | 30°C and glyine/NaOH buffer, pH 9.5 | Rhodococcus erythropolis | |
4.9 | - |
(1R,4S)-1-hydroxy-2-oxolimonene | 30°C and glyine/NaOH buffer, pH 9.5 | Rhodococcus erythropolis | |
6 | - |
(1S,4R)-menthone | 30°C and glyine/NaOH buffer, pH 9.5 | Rhodococcus erythropolis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.3 | - |
pH-optimum in Tris/HCl buffer | Rhodococcus erythropolis |
9.5 | - |
pH-optimum in glycine/NaOH buffer | Rhodococcus erythropolis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
4 min, inactivation of MMKMO in Tris/HCl buffer is observed above pH 8.5 | Rhodococcus erythropolis |
10 | - |
4 min, inactivation of MMKMO in glycine/NaOH buffer is observed above pH 8.5 | Rhodococcus erythropolis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | contains 1 mol FAD per monomer as prosthetic group | Rhodococcus erythropolis | |
NADPH | no activity with NADH | Rhodococcus erythropolis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
9.5 | - |
ATP | - |
Rhodococcus erythropolis |