KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0013 | - |
NADPH | pH 7.0, pH 37°C | Tyria jacobaeae | |
0.0014 | - |
senecionine | pH 7.0, 37°C | Tyria jacobaeae | |
0.0029 | - |
monocrotaline | pH 7.0, 37°C | Creatonotos transiens | |
0.0031 | - |
senecionine | pH 7.0, 37°C | Creatonotos transiens | |
0.0048 | - |
senecionine | pH 7.0, 37°C | Arctia caja | |
0.0051 | - |
monocrotaline | pH 7.0, 37°C | Arctia caja | |
0.0125 | - |
monocrotaline | pH 7.0, 37°C | Tyria jacobaeae | |
0.0186 | - |
heliotrine | pH 7.0, 37°C | Arctia caja | |
0.0229 | - |
heliotrine | pH 7.0, 37°C | Creatonotos transiens | |
0.284 | - |
heliotrine | pH 7.0, 37°C | Tyria jacobaeae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Tyria jacobaeae | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
51000 | - |
4 * 51000, SDS-PAGE | Tyria jacobaeae |
200000 | - |
gel filtration | Tyria jacobaeae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Amerila phaedra | - |
- |
- |
Arctia caja | - |
- |
- |
Arctia intercalaris | - |
- |
- |
Callimorpha dominula | - |
- |
- |
Creatonotos transiens | - |
- |
- |
Idea leuconoe | - |
- |
- |
no activity in Spodoptera littoralis | - |
- |
- |
Tyria jacobaeae | - |
- |
- |
Zonocerus variegatus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Tyria jacobaeae |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adult | - |
Amerila phaedra | - |
fat body | - |
Zonocerus variegatus | - |
hemolymph | from late instar larvae | Tyria jacobaeae | - |
larva | - |
Arctia caja | - |
larva | - |
Creatonotos transiens | - |
larva | - |
Callimorpha dominula | - |
larva | - |
Arctia intercalaris | - |
pupa | - |
Idea leuconoe | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4.67 | - |
- |
Tyria jacobaeae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
axillaridine + NADPH + O2 | 76% of the activity with senecionine | Tyria jacobaeae | axillaridine N-oxide + NADP+ + H2O | - |
? | |
axillaridine + NADPH + O2 | 79% of the activity with senecionine | Arctia caja | axillaridine N-oxide + NADP+ + H2O | - |
? | |
axillaridine + NADPH + O2 | 93% of the activity with senecionine | Creatonotos transiens | axillaridine N-oxide + NADP+ + H2O | - |
? | |
axillarine + NADPH + O2 | 38% of the activity with senecionine | Creatonotos transiens | axillarine N-oxide + NADP+ + H2O | - |
? | |
axillarine + NADPH + O2 | 55% of the activity with senecionine | Arctia caja | axillarine N-oxide + NADP+ + H2O | - |
? | |
axillarine + NADPH + O2 | 74% of the activity with senecionine | Tyria jacobaeae | axillarine N-oxide + NADP+ + H2O | - |
? | |
heliotrine + NADPH + O2 | - |
Tyria jacobaeae | heliotrine N-oxide + NADP+ + H2O | - |
? | |
heliotrine + NADPH + O2 | 115% of the activity with senecionine | Creatonotos transiens | heliotrine N-oxide + NADP+ + H2O | - |
? | |
heliotrine + NADPH + O2 | 25% of the activity with senecionine | Tyria jacobaeae | heliotrine N-oxide + NADP+ + H2O | - |
? | |
heliotrine + NADPH + O2 | 86% of the activity with senecionine | Arctia caja | heliotrine N-oxide + NADP+ + H2O | - |
? | |
indicine + NADPH + O2 | 35% of the activity with senecionine | Tyria jacobaeae | indicine N-oxide + NADP+ + H2O | - |
? | |
indicine + NADPH + O2 | 78% of the activity with senecionine | Creatonotos transiens | indicine N-oxide + NADP+ + H2O | - |
? | |
indicine + NADPH + O2 | 90% of the activity with senecionine | Arctia caja | indicine N-oxide + NADP+ + H2O | - |
? | |
lycopsamine + NADPH + O2 | 20% of the activity with senecionine | Tyria jacobaeae | lycopsamine N-oxide + NADP+ + H2O | - |
? | |
lycopsamine + NADPH + O2 | 75% of the activity with senecionine | Creatonotos transiens | lycopsamine N-oxide + NADP+ + H2O | - |
? | |
lycopsamine + NADPH + O2 | 93% of the activity with senecionine | Arctia caja | lycopsamine N-oxide + NADP+ + H2O | - |
? | |
monocrotaline + NADPH + O2 | - |
Tyria jacobaeae | monocrotaline N-oxide + NADP+ + H2O | - |
? | |
monocrotaline + NADPH + O2 | 103% of the activity with senecionine | Arctia caja | monocrotaline N-oxide + NADP+ + H2O | - |
? | |
monocrotaline + NADPH + O2 | 110% of the activity with senecionine | Creatonotos transiens | monocrotaline N-oxide + NADP+ + H2O | - |
? | |
monocrotaline + NADPH + O2 | 92% of the activity with senecionine | Tyria jacobaeae | monocrotaline N-oxide + NADP+ + H2O | - |
? | |
additional information | the enzyme N-oxidizes only alkaloids with structural elements which are essential for hepatotoxic and genotoxic pyrrolizidine alkaloids, i.e. 1,2-double bond, esterification of the allylic hydroxyl group, presence of a second free or esterified hydroxyl group at carbon 7. No activity with: senkirkine, sarracine, supinine, phalaenopsine, retronecine, heliotridine, supinidine, isoretronecanol, nicotine, caffeine, tropane alkaloids, quinolizidine alkaloids, indole alkaloids, isoquinolines and synthetic heterocyclic tertiary amines | Tyria jacobaeae | ? | - |
? | |
additional information | the enzyme N-oxidizes only alkaloids with structural elements which are essential for hepatotoxic and genotoxic pyrrolizidine alkaloids, i.e. 1,2-double bond, esterification of the allylic hydroxyl group, presence of a second free or esterified hydroxyl group at carbon 7. No activity with: triangularine, senkirkine, sarracine, supinine, phalaenopsine, retronecine, heliotridine, supinidine, isoretronecanol, nicotine, caffeine, tropane alkaloids, quinolizidine alkaloids, indole alkaloids, isoquinolines and synthetic heterocyclic tertiary amines | Arctia caja | ? | - |
? | |
additional information | the enzyme N-oxidizes only alkaloids with structural elements which are essential for hepatotoxic and genotoxic pyrrolizidine alkaloids, i.e. 1,2-double bond, esterification of the allylic hydroxyl group, presence of a second free or esterified hydroxyl group at carbon 7. No activity with: triangularine, senkirkine, sarracine, supinine, phalaenopsine, retronecine, heliotridine, supinidine, isoretronecanol, nicotine, caffeine, tropane alkaloids, quinolizidine alkaloids, indole alkaloids, isoquinolines and synthetic heterocyclic tertiary amines | Creatonotos transiens | ? | - |
? | |
retrorsine + NADPH + O2 | 93% of the activity with senecionine | Creatonotos transiens | retrorsine N-oxide + NADP+ + H2O | - |
? | |
retrorsine + NADPH + O2 | 97% of the activity with senecionine | Tyria jacobaeae | retrorsine N-oxide + NADP+ + H2O | - |
? | |
retrorsine + NADPH + O2 | as active as senecionine | Arctia caja | retrorsine N-oxide + NADP+ + H2O | - |
? | |
rinderine + NADPH + O2 | 23% of the activity with senecionine | Tyria jacobaeae | rinderine N-oxide + NADP+ + H2O | - |
? | |
rinderine + NADPH + O2 | 79% of the activity with senecionine | Arctia caja | rinderine N-oxide + NADP+ + H2O | - |
? | |
rinderine + NADPH + O2 | 81% of the activity with senecionine | Creatonotos transiens | rinderine N-oxide + NADP+ + H2O | - |
? | |
senecionine + NADPH + O2 | - |
Arctia caja | senecionine N-oxide + NADP+ + H2O | - |
? | |
senecionine + NADPH + O2 | - |
Tyria jacobaeae | senecionine N-oxide + NADP+ + H2O | - |
? | |
senecionine + NADPH + O2 | - |
Creatonotos transiens | senecionine N-oxide + NADP+ + H2O | - |
? | |
seneciphylline + NADPH + O2 | 117% of the activity with senecionine | Arctia caja | seneciphylline N-oxide + NADP+ + H2O | - |
? | |
seneciphylline + NADPH + O2 | 119% of the activity with senecionine | Creatonotos transiens | seneciphylline N-oxide + NADP+ + H2O | - |
? | |
seneciphylline + NADPH + O2 | 95% of the activity with senecionine | Tyria jacobaeae | seneciphylline N-oxide + NADP+ + H2O | - |
? | |
senecivernine + NADPH + O2 | 107% of the activity with senecionine | Arctia caja | senecivernine N-oxide + NADP+ + H2O | - |
? | |
senecivernine + NADPH + O2 | 113% of the activity with senecionine | Creatonotos transiens | senecivernine N-oxide + NADP+ + H2O | - |
? | |
senecivernine + NADPH + O2 | 81% of the activity with senecionine | Tyria jacobaeae | senecivernine N-oxide + NADP+ + H2O | - |
? | |
triangularine + NADPH + O2 | 60% of the activity with senecionine | Tyria jacobaeae | triangularine N-oxide + NADP+ + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 51000, SDS-PAGE | Tyria jacobaeae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 45 | - |
Tyria jacobaeae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Tyria jacobaeae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | molar apoenzyme/FAD ratio of 0.4 | Tyria jacobaeae | |
NADPH | strictly dependent on | Arctia caja | |
NADPH | strictly dependent on | Creatonotos transiens | |
NADPH | strictly dependent on, no reaction with NADH | Tyria jacobaeae |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Tyria jacobaeae | determination on an Ampholine PAGplate pH 3.5-9.5 using a horizantal electrophoresis system | - |
4.9 |