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Literature summary for 1.14.13.1 extracted from

  • White-Stevens, R.H.; Kamin, H.
    Studies of a flavoprotein, salicylate hydroxylase. I. Preparation, properties, and the uncoupling of oxygen reduction from hydroxylation (1972), J. Biol. Chem., 247, 2358-2370.
    View publication on PubMed
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Inhibitors

Inhibitors Comment Organism Structure
benzoate competitive inhibitor Pseudomonas sp.
Br-
-
 Pseudomonas sp.
Cl-
-
 Pseudomonas sp.
F-
-
 Pseudomonas sp.
I-
-
 Pseudomonas sp.
NO3-
-
 Pseudomonas sp.
p-chloromercuribenzoate
-
 Pseudomonas sp.
SCN-
-
 Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Pseudomonas sp.
0.0027
-
 salicylate
-
 Pseudomonas sp.
0.011
-
 2,6-dihydroxybenzoate
-
 Pseudomonas sp.
0.015
-
 p-Aminosalicylate
-
 Pseudomonas sp.
0.0167
-
 NADH
-
 Pseudomonas sp.
0.017
-
 NADH
-
 Pseudomonas sp.
0.028
-
 2,3-Dihydroxybenzoate
-
 Pseudomonas sp.
0.065
-
 2,5-Dihydroxybenzoate
-
 Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000 48700 gel filtration, sedimentation equilibrium, calculation of MW per flavin Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
10.57
-
-
 Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,3-dihydroxybenzoate + NADH + O2
-
 Pseudomonas sp. pyrogallol + CO2 + H2O + NAD+
-
 ?
2,5-dihydroxybenzoate + NADH + O2
-
 Pseudomonas sp. benzene-1,2,5-triol + CO2 + H2O + NAD+
-
 ?
2,6-dihydroxybenzoate + NADH + O2
-
 Pseudomonas sp. pyrogallol + CO2 + H2O + NAD+
-
 ?
m-hydroxybenzoate + NADH + O2 6% of the reaction with salicylate Pseudomonas sp. 1,3-dihydroxybenzene + CO2 + NAD+ + H2O
-
 ?
p-aminosalicylate + NADH + O2
-
 Pseudomonas sp. 1,2-dihydroxy-4-aminobenzene + NAD+ + CO2 + H2O
-
 ?
salicylate + NADH + H+ + O2
-
 Pseudomonas sp. catechol + NAD+ + H2O + CO2
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 43000-48700, sedimentation equilibrium Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8.5
-
 Pseudomonas sp.

Cofactor

Cofactor Comment Organism Structure
FAD flavoprotein Pseudomonas sp.
NADH
-
 Pseudomonas sp.

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1
-
 benzoate
-
 Pseudomonas sp.
60
-
 Cl-
-
 Pseudomonas sp.