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Literature summary for 1.14.13.1 extracted from

  • Yamamoto, S.; Katagiri, M.; Maeno, H.; Hayaishi, O.
    Salicylate hydroxylase, a monooxygenase requiring flavin adenine dinucleotide (1965), J. Biol. Chem., 240, 3408-3413.
    View publication on PubMed
Search for more literature for 1.14.13.1

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
 Pseudomonas sp.
ascorbic acid slight Pseudomonas sp.
CuSO4
-
 Pseudomonas sp.
Na2MoO4 slight Pseudomonas sp.
p-chloromercuribenzoate
-
 Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0019
-
 salicylate
-
 Pseudomonas sp.
0.0026
-
 NADH
-
 Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57200
-
 calculation from diffusion and sedimentation data Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
13.5
-
-
 Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,3-dihydroxybenzoate + NADH + O2
-
 Pseudomonas sp. pyrogallol + CO2 + H2O + NAD+
-
 ?
2,6-dihydroxybenzoate + NADH + O2
-
 Pseudomonas sp. pyrogallol + CO2 + H2O + NAD+
-
 ?
salicylate + NADH + H+ + O2
-
 Pseudomonas sp. catechol + NAD+ + H2O + CO2
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20
-
 assay at Pseudomonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25
-
 apoenzyme unstable above Pseudomonas sp.
38
-
 30 min, complete inactivation in absence of FAD Pseudomonas sp.
38
-
 2 h, 10% loss of activity in the presence of FAD Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8
-
 Pseudomonas sp.

pH Range

pH Minimum pH Maximum Comment Organism
6 10 about 50% of activity maximum at pH 6 and 10 Pseudomonas sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
8
-
 highest stability Pseudomonas sp.

Cofactor

Cofactor Comment Organism Structure
FAD flavoprotein Pseudomonas sp.
FAD 1 mol of FAD loosly bound to 1 mol of enzyme, MW 57000 Pseudomonas sp.
NADH
-
 Pseudomonas sp.