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Literature summary for 1.14.12.8 extracted from

  • Junker, F.; Saller, E.; Schläfli Oppenberg, H.R.; Kroneck, P.M.H.; Leisinger, T.; Cook, A.M.
    Degradative pathways for p-toluenecarboxylate and p-toluenesulfonate and their multicomponent oxygenases in Comamonas testosteroni strains PSB-4 and T-2 (1996), Microbiology, 142, 2419-2427.
    View publication on PubMed

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+
-
Comamonas testosteroni

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
dioxygenase A, SDS-PAGE Comamonas testosteroni

Organism

Organism UniProt Comment Textmining
Comamonas testosteroni
-
T-2
-
Comamonas testosteroni
-
PSB-4
-
Comamonas testosteroni PSB-4
-
PSB-4
-
Comamonas testosteroni T-2
-
T-2
-

Purification (Commentary)

Purification (Comment) Organism
purification of the oxygenase system Comamonas testosteroni

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.03
-
for enzyme system grown on sulfobenzoate Comamonas testosteroni

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-sulfobenzoate + NADH + O2
-
Comamonas testosteroni 3,4-dihydroxybenzoate + sulfite + NAD+
-
?
4-sulfobenzoate + NADH + O2
-
Comamonas testosteroni PSB-4 3,4-dihydroxybenzoate + sulfite + NAD+
-
?
4-sulfobenzoate + NADH + O2
-
Comamonas testosteroni T-2 3,4-dihydroxybenzoate + sulfite + NAD+
-
?
4-sulfobenzoate + NADH + O2
-
Comamonas testosteroni TER-1: 3,4-dihydroxybenzoate + sulfite + NAD+
-
?
4-toluenesulfonate + NADH + O2 this strain requires the reductase (TsaB) of toluenesulfonate methyl monooxygenase in the incompletely expressed sulfobenzoate dioxygenase system (PsbAC) Comamonas testosteroni ?
-
?
4-toluenesulfonate + NADH + O2 this strain requires the reductase (TsaB) of toluenesulfonate methyl monooxygenase in the incompletely expressed sulfobenzoate dioxygenase system (PsbAC) Comamonas testosteroni PSB-4 ?
-
?
4-toluenesulfonate + NADH + O2 this strain requires the reductase (TsaB) of toluenesulfonate methyl monooxygenase in the incompletely expressed sulfobenzoate dioxygenase system (PsbAC) Comamonas testosteroni T-2 ?
-
?
4-toluenesulfonate + NADH + O2 this strain requires the reductase (TsaB) of toluenesulfonate methyl monooxygenase in the incompletely expressed sulfobenzoate dioxygenase system (PsbAC) Comamonas testosteroni TER-1: ?
-
?