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Literature summary for 1.14.12.7 extracted from

  • Batie, C.J.; LaHaie, E.; Ballou, D.P.
    Purification and characterization of phthalate oxygenase and phthalate oxygenase reductase from Pseudomonas cepacia (1987), J. Biol. Chem., 262, 1510-1518.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
Burkholderia cepacia
Cu2+
-
Burkholderia cepacia
EDTA
-
Burkholderia cepacia
Zn2+
-
Burkholderia cepacia

Metals/Ions

Metals/Ions Comment Organism Structure
Fe3+ stimulates Burkholderia cepacia
Iron active form of enzyme contains one mononuclear iron and one [2Fe-2S] center per monomer Burkholderia cepacia

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48000
-
4 * 48000, SDS-PAGE Burkholderia cepacia
217000
-
gel filtration Burkholderia cepacia

Organism

Organism UniProt Comment Textmining
Burkholderia cepacia
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,3-dicarboxypyridine + NADH + O2 69% of the activity with o-phthalate Burkholderia cepacia 5,6-dihydroxy-5,6-dihydropyridine-2,3-dicarboxylate + NAD+ + H2O
-
?
4-chloro-o-phthalate + NADH + O2 79% of the activity with o-phthalate Burkholderia cepacia 4-chloro-cis-4,5-dihydroxycyclohexa-1(6),2-diene-1,2-dicarboxylate + NAD+ + H2O
-
?
phthalate + NADH + O2 o-phthalate Burkholderia cepacia cis-4,5-dihydroxycyclohexa-1(6),2-diene-1,2-dicarboxylate + NAD+ + H2O
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 48000, SDS-PAGE Burkholderia cepacia

Cofactor

Cofactor Comment Organism Structure
FMN enzyme contains FMN Burkholderia cepacia
NADH
-
Burkholderia cepacia