Cloned (Comment) | Organism |
---|---|
genes bedC1 and bedC2 encoding the terminal oxygenase alpha-subunit and beta-subunit, expression in Escherichia coli | Pseudomonas putida |
Protein Variants | Comment | Organism |
---|---|---|
H119C | the mutant alpha-subunit of the terminal dioxygenase is unable to coordinate an EPR-detectable Rieske [2Fe-2S] cluster with the characteristic g factors | Pseudomonas putida |
H222M | in the reconstitution assay with the reductase component, the ferredoxin component and the beta-subunit of terminal dioxygenase the mutant alpha-subunit is unable to reconstitute dioxygenase activity | Pseudomonas putida |
H228C | in the reconstitution assay with the reductase component, the ferredoxin component and the beta-subunit of terminal dioxygenase the mutant alpha-subunit is unable to reconstitute dioxygenase activity | Pseudomonas putida |
H98C | the mutant alpha-subunit of the terminal dioxygenase is unable to coordinate an EPR-detectable Rieske [2Fe-2S] cluster with the characteristic g factors, detection of a novel EPR spectrum, the intensity of the spectrum is approximately 8% from the wild-type | Pseudomonas putida |
Y118S | the mutant alpha-subunit of the terminal dioxygenase shows an EPR spectrum of half the intensity of that of the wild-type. In the reconstitution assay with the reductase component, the ferredoxin component and the beta-subunit of terminal dioxygenase it shows significantly reduced activities | Pseudomonas putida |
Y221A | in the reconstitution assay with the reductase component, the ferredoxin component and the beta-subunit of terminal dioxygenase the mutant alpha-subunit shows significantly reduced activity | Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
55000 Da is the MW of the alpha-subunit of the terminal dioxygenase | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzene + NADH + O2 | Pseudomonas putida | reaction in benzene catabolism | ? | - |
? | |
benzene + NADH + O2 | Pseudomonas putida ML2 | reaction in benzene catabolism | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Pseudomonas putida ML2 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzene + NADH + O2 | - |
Pseudomonas putida | cis-cyclohexa-3,5-diene-1,2-diol + NAD+ | - |
? | |
benzene + NADH + O2 | - |
Pseudomonas putida ML2 | cis-cyclohexa-3,5-diene-1,2-diol + NAD+ | - |
? | |
benzene + NADH + O2 | reaction in benzene catabolism | Pseudomonas putida | ? | - |
? | |
benzene + NADH + O2 | reaction in benzene catabolism | Pseudomonas putida ML2 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | 55000 Da is the MW of the alpha-subunit of the terminal dioxygenase | Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
Ferredoxin | the benzene dioxygenase comprises three components: 1. flavoprotein, NADH:ferredoxin oxidoreductase, 2. an intermediate electron-transfer protein, or ferredoxin and 3. a terminal dioxygenase | Pseudomonas putida |