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Literature summary for 1.14.12.3 extracted from

  • Axcell, B.C.; Geary, P.J.
    Purification and some properties of a soluble benzene-oxidizing system from a strain of Pseudomonas (1975), Biochem. J., 146, 173-183.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.12.3

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0112
-
 NADH
-
 Pseudomonas sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
 Pseudomonas sp.
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ required Pseudomonas sp.
Iron the terminal dioxygenase component contains 2 atoms of iron and 3 atoms of inorganic sulfur Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
186000
-
 terminal dioxygenase component, meniscus depletion method Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
benzene + NADH + O2 Pseudomonas sp. reaction in benzene catabolism ?
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzene + NADH + O2
-
 Pseudomonas sp. cis-cyclohexa-3,5-diene-1,2-diol + NAD+
-
 ?
benzene + NADH + O2 reaction in benzene catabolism Pseudomonas sp. ?
-
 ?
benzene + NADPH + O2 activity with NADPH is 10% of the activity with NADH Pseudomonas sp. cis-cyclohexa-3,5-diene-1,2-diol + NADP+
-
 ?

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Pseudomonas sp.
NADPH activity with NADPH is 10% of the activity with NADH Pseudomonas sp.