Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli JM109(DE3) cells | Burkholderia sp. |
Protein Variants | Comment | Organism |
---|---|---|
additional information | engineering of hybrid dioxygenase enzymes coexpressing genes from naphthalene and 2,4-dinitrotoluene dioxygenases in Escherichia coli. In the active hybrids, replacement of small subunits affects the rate of product formation but has no effect on the substrate range, regiospecificity, or enantiomeric purity of oxidation products with the substrates tested. The small subunit of the oxygenase is essential for activity but does not play a major role in determining the specificity of these enzymes. Introduction of the small subunit of 2-nitrotolene synthase from Pseudomonas sp. strain JS42 leads to 30% reduction in product formation from naphthalene and dinitrotoluene | Burkholderia sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
x * 50000, SDS-PAGE | Burkholderia sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,4-dinitrotoluene + NADH + H+ + O2 | Burkholderia sp. | - |
4-methyl-5-nitrocatechol + nitrite + NAD+ | - |
? | |
2,4-dinitrotoluene + NADH + H+ + O2 | Burkholderia sp. DNT | - |
4-methyl-5-nitrocatechol + nitrite + NAD+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Burkholderia sp. | - |
- |
- |
Burkholderia sp. | Q5BM20 and Q6QUY4 and Q45695 and Q6QUY3 | Q5BM20 i.e flavoprotein reductase DntAa, Q6QUY4 i.e iron-sulfur ferredoxin DntAb, Q45695 i.e. large subunit DntAc, Q6QUY3 i.e. small subunit DntAd | - |
Burkholderia sp. DNT | - |
- |
- |
Burkholderia sp. DNT | Q5BM20 and Q6QUY4 and Q45695 and Q6QUY3 | Q5BM20 i.e flavoprotein reductase DntAa, Q6QUY4 i.e iron-sulfur ferredoxin DntAb, Q45695 i.e. large subunit DntAc, Q6QUY3 i.e. small subunit DntAd | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.38 | - |
with naphthalene as substrate, pH and temperature not specified in the publication | Burkholderia sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,4-dinitrotoluene + NADH + H+ + O2 | - |
Burkholderia sp. | 4-methyl-5-nitrocatechol + nitrite + NAD+ | - |
? | |
2,4-dinitrotoluene + NADH + H+ + O2 | - |
Burkholderia sp. DNT | 4-methyl-5-nitrocatechol + nitrite + NAD+ | - |
? | |
2-nitrotoluene + NADH + H+ + O2 | - |
Burkholderia sp. | 2-nitrobenzyl alcohol + NAD+ + ? | - |
? | |
2-nitrotoluene + NADH + H+ + O2 | - |
Burkholderia sp. DNT | 2-nitrobenzyl alcohol + NAD+ + ? | - |
? | |
naphthalene + NADH + H+ + O2 | - |
Burkholderia sp. | (+)-cis-(1R,2S)-dihydroxy-1,2-dihydronaphthalene + NAD+ + ? | - |
? | |
naphthalene + NADH + H+ + O2 | - |
Burkholderia sp. DNT | (+)-cis-(1R,2S)-dihydroxy-1,2-dihydronaphthalene + NAD+ + ? | - |
? | |
naphthalene + NADH + H+ + O2 | - |
Burkholderia sp. | (1R,2S)-cis-1,2-dihydroxy-1,2-dihydronaphthalene + NAD+ | - |
? | |
naphthalene + NADH + H+ + O2 | - |
Burkholderia sp. DNT | (1R,2S)-cis-1,2-dihydroxy-1,2-dihydronaphthalene + NAD+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 50000, SDS-PAGE | Burkholderia sp. |
Synonyms | Comment | Organism |
---|---|---|
DNTDO | - |
Burkholderia sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Burkholderia sp. |