BRENDA - Enzyme Database show
show all sequences of 1.14.12.24

Substrate specificities of hybrid naphthalene and 2,4-dinitrotoluene dioxygenase enzyme systems

Parales, R.E.; Emig, M.D.; Lynch, N.A.; Gibson, D.T.; J. Bacteriol. 180, 2337-2344 (1998)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli JM109(DE3) cells
Burkholderia sp.
Engineering
Amino acid exchange
Commentary
Organism
additional information
engineering of hybrid dioxygenase enzymes coexpressing genes from naphthalene and 2,4-dinitrotoluene dioxygenases in Escherichia coli. In the active hybrids, replacement of small subunits affects the rate of product formation but has no effect on the substrate range, regiospecificity, or enantiomeric purity of oxidation products with the substrates tested. The small subunit of the oxygenase is essential for activity but does not play a major role in determining the specificity of these enzymes. Introduction of the small subunit of 2-nitrotolene synthase from Pseudomonas sp. strain JS42 leads to 30% reduction in product formation from naphthalene and dinitrotoluene
Burkholderia sp.
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
50000
-
x * 50000, SDS-PAGE
Burkholderia sp.
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2,4-dinitrotoluene + NADH + H+ + O2
Burkholderia sp.
-
4-methyl-5-nitrocatechol + nitrite + NAD+
-
-
?
2,4-dinitrotoluene + NADH + H+ + O2
Burkholderia sp. DNT
-
4-methyl-5-nitrocatechol + nitrite + NAD+
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Burkholderia sp.
-
-
-
Burkholderia sp.
Q5BM20 and Q6QUY4 and Q45695 and Q6QUY3
Q5BM20 i.e flavoprotein reductase DntAa, Q6QUY4 i.e iron-sulfur ferredoxin DntAb, Q45695 i.e. large subunit DntAc, Q6QUY3 i.e. small subunit DntAd
-
Burkholderia sp. DNT
-
-
-
Burkholderia sp. DNT
Q5BM20 and Q6QUY4 and Q45695 and Q6QUY3
Q5BM20 i.e flavoprotein reductase DntAa, Q6QUY4 i.e iron-sulfur ferredoxin DntAb, Q45695 i.e. large subunit DntAc, Q6QUY3 i.e. small subunit DntAd
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
0.38
-
with naphthalene as substrate, pH and temperature not specified in the publication
Burkholderia sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2,4-dinitrotoluene + NADH + H+ + O2
-
734058
Burkholderia sp.
4-methyl-5-nitrocatechol + nitrite + NAD+
-
-
-
?
2,4-dinitrotoluene + NADH + H+ + O2
-
734058
Burkholderia sp. DNT
4-methyl-5-nitrocatechol + nitrite + NAD+
-
-
-
?
2-nitrotoluene + NADH + H+ + O2
-
734058
Burkholderia sp.
2-nitrobenzyl alcohol + NAD+ + ?
-
-
-
?
2-nitrotoluene + NADH + H+ + O2
-
734058
Burkholderia sp. DNT
2-nitrobenzyl alcohol + NAD+ + ?
-
-
-
?
naphthalene + NADH + H+ + O2
-
734058
Burkholderia sp.
(+)-cis-(1R,2S)-dihydroxy-1,2-dihydronaphthalene + NAD+ + ?
-
-
-
?
naphthalene + NADH + H+ + O2
-
734058
Burkholderia sp. DNT
(+)-cis-(1R,2S)-dihydroxy-1,2-dihydronaphthalene + NAD+ + ?
-
-
-
?
naphthalene + NADH + H+ + O2
-
734058
Burkholderia sp.
(1R,2S)-cis-1,2-dihydroxy-1,2-dihydronaphthalene + nitrite + NAD+
-
-
-
?
naphthalene + NADH + H+ + O2
-
734058
Burkholderia sp. DNT
(1R,2S)-cis-1,2-dihydroxy-1,2-dihydronaphthalene + nitrite + NAD+
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 50000, SDS-PAGE
Burkholderia sp.
Cofactor
Cofactor
Commentary
Organism
Structure
NADH
-
Burkholderia sp.
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli JM109(DE3) cells
Burkholderia sp.
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADH
-
Burkholderia sp.
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
engineering of hybrid dioxygenase enzymes coexpressing genes from naphthalene and 2,4-dinitrotoluene dioxygenases in Escherichia coli. In the active hybrids, replacement of small subunits affects the rate of product formation but has no effect on the substrate range, regiospecificity, or enantiomeric purity of oxidation products with the substrates tested. The small subunit of the oxygenase is essential for activity but does not play a major role in determining the specificity of these enzymes. Introduction of the small subunit of 2-nitrotolene synthase from Pseudomonas sp. strain JS42 leads to 30% reduction in product formation from naphthalene and dinitrotoluene
Burkholderia sp.
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
50000
-
x * 50000, SDS-PAGE
Burkholderia sp.
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2,4-dinitrotoluene + NADH + H+ + O2
Burkholderia sp.
-
4-methyl-5-nitrocatechol + nitrite + NAD+
-
-
?
2,4-dinitrotoluene + NADH + H+ + O2
Burkholderia sp. DNT
-
4-methyl-5-nitrocatechol + nitrite + NAD+
-
-
?
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
0.38
-
with naphthalene as substrate, pH and temperature not specified in the publication
Burkholderia sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2,4-dinitrotoluene + NADH + H+ + O2
-
734058
Burkholderia sp.
4-methyl-5-nitrocatechol + nitrite + NAD+
-
-
-
?
2,4-dinitrotoluene + NADH + H+ + O2
-
734058
Burkholderia sp. DNT
4-methyl-5-nitrocatechol + nitrite + NAD+
-
-
-
?
2-nitrotoluene + NADH + H+ + O2
-
734058
Burkholderia sp.
2-nitrobenzyl alcohol + NAD+ + ?
-
-
-
?
2-nitrotoluene + NADH + H+ + O2
-
734058
Burkholderia sp. DNT
2-nitrobenzyl alcohol + NAD+ + ?
-
-
-
?
naphthalene + NADH + H+ + O2
-
734058
Burkholderia sp.
(+)-cis-(1R,2S)-dihydroxy-1,2-dihydronaphthalene + NAD+ + ?
-
-
-
?
naphthalene + NADH + H+ + O2
-
734058
Burkholderia sp. DNT
(+)-cis-(1R,2S)-dihydroxy-1,2-dihydronaphthalene + NAD+ + ?
-
-
-
?
naphthalene + NADH + H+ + O2
-
734058
Burkholderia sp.
(1R,2S)-cis-1,2-dihydroxy-1,2-dihydronaphthalene + nitrite + NAD+
-
-
-
?
naphthalene + NADH + H+ + O2
-
734058
Burkholderia sp. DNT
(1R,2S)-cis-1,2-dihydroxy-1,2-dihydronaphthalene + nitrite + NAD+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 50000, SDS-PAGE
Burkholderia sp.
Other publictions for EC 1.14.12.24
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [░C]
Temperature Range [░C]
Temperature Stability [░C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [░C] (protein specific)
Temperature Range [░C] (protein specific)
Temperature Stability [░C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
741610
Kuece
-
Biodegradation of 2,4-dinitro ...
Microbacterium saccharophilum
Ann. Microbiol.
65
467-476
2015
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
733523
Khleifat
-
Characterization of 2,4-dinitr ...
Burkholderia sp., Burkholderia sp. DNT
Bioremediat. J.
14
38-53
2010
9
-
1
-
-
-
6
2
-
1
1
2
-
8
-
-
1
-
-
-
2
-
2
1
1
1
2
-
1
1
-
4
-
-
-
9
-
1
4
-
-
-
-
6
-
2
-
1
1
2
-
-
-
1
-
-
2
-
2
1
1
1
2
-
1
1
-
-
-
-
-
-
-
-
733549
Leungsakul T
Oxidation of aminonitrotoluene ...
Burkholderia sp., Burkholderia sp. DNT
Biotechnol. Bioeng.
93
231-237
2006
-
-
1
-
1
-
-
-
-
-
-
2
-
16
-
-
-
-
-
-
-
-
27
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
27
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
733548
Leungsakul
Saturation mutagenesis of 2,4- ...
Burkholderia sp., Burkholderia sp. DNT
Biotechnol. Bioeng.
92
416-426
2005
-
-
1
-
2
-
-
-
-
-
-
4
-
5
-
-
-
-
-
-
-
-
11
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
11
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
733152
Keenan
Saturation mutagenesis of Burk ...
Burkholderia cepacia, Burkholderia cepacia R34, Burkholderia sp., Burkholderia sp. DNT
Appl. Environ. Microbiol.
70
3222-3231
2004
-
-
1
-
4
-
-
-
-
-
-
2
-
9
-
-
-
-
-
-
-
-
24
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
4
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
24
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
734412
Lin
Effects of Vitreoscilla hemogl ...
Burkholderia sp., Burkholderia sp. DNT
J. Ind. Microbiol. Biotechnol.
30
362-368
2003
1
-
-
-
-
-
-
1
1
-
2
2
-
11
-
-
1
-
-
-
2
-
2
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
1
-
2
2
-
-
-
1
-
-
2
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
734502
Fish P.A
-
Vitreoscilla hemoglobin enhanc ...
Burkholderia sp., Burkholderia sp. DNT
J. Mol. Catal. B
9
75-82
2000
1
-
1
-
-
-
-
-
-
-
-
2
-
8
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
734057
Parales
Enzyme specificity of 2-nitrot ...
Burkholderia sp., Burkholderia sp. DNT
J. Bacteriol.
180
1194-1199
1998
-
-
-
-
1
-
-
-
-
-
-
-
-
11
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
734058
Parales
Substrate specificities of hyb ...
Burkholderia sp., Burkholderia sp. DNT
J. Bacteriol.
180
2337-2344
1998
-
-
1
-
1
-
-
-
-
-
1
2
-
14
-
-
-
-
-
-
1
-
10
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
-
1
2
-
-
-
-
-
-
1
-
10
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
734055
Suen
2,4-Dinitrotoluene dioxygenase ...
Burkholderia sp., Burkholderia sp. DNT
J. Bacteriol.
178
4926-4934
1996
-
-
1
-
-
-
-
-
-
-
-
2
-
16
-
-
-
-
-
-
-
-
25
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
25
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
734052
Suen
Cloning and characterization o ...
Pseudomonas sp., Pseudomonas sp. DNT
J. Bacteriol.
175
1831-1837
1993
-
-
-
-
-
-
-
-
-
-
-
2
-
5
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
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-
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-
-
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-
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-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
733145
Spanggord
Biodegradation of 2,4-dinitrot ...
Pseudomonas sp., Pseudomonas sp. DNT
Appl. Environ. Microbiol.
57
3200-3205
1991
-
-
-
-
-
-
-
-
-
-
-
2
-
4
-
-
-
-
-
-
-
-
12
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
12
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-