Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.12.24 extracted from

  • Suen, W.C.; Haigler, B.E.; Spain, J.C.
    2,4-Dinitrotoluene dioxygenase from Burkholderia sp. strain DNT: similarity to naphthalene dioxygenase (1996), J. Bacteriol., 178, 4926-4934.
    View publication on PubMed
Search for more literature for 1.14.12.24

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli JM109(DE3) cells Burkholderia sp.
expression in Escherichia coli Burkholderia sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2,4-dinitrotoluene + NADH + O2 Burkholderia sp.
-
 4-methyl-5-nitrocatechol + nitrite + NAD+
-
 ?

Organism

Organism UniProt Comment Textmining
Burkholderia sp.
-
-
-
Burkholderia sp. Q5BM20 and Q6QUY4 and Q45695 and Q6QUY3 Q5BM20 i.e flavoprotein reductase DntAa, Q6QUY4 i.e iron-sulfur ferredoxin DntAb, Q45695 i.e. large subunit DntAc, Q6QUY3 i.e. small subunit DntAd
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,4-dinitrotoluene + NADH + O2
-
 Burkholderia sp. 4-methyl-5-nitrocatechol + nitrite + NAD+
-
 ?
2-nitrotoluene + NADH + H+ + O2
-
 Burkholderia sp. 2-nitrobenzyl alcohol + NAD+ + ?
-
 ?
3-nitrotoluene + NADH + H+ + O2
-
 Burkholderia sp. 3-nitrobenzyl alcohol + NAD+ + ?
-
 ?
4-nitrotoluene + NADH + H+ + O2
-
 Burkholderia sp. 4-nitrobenzyl alcohol + NAD+ + ?
-
 ?
acenaphthene + NADH + H+ + O2
-
 Burkholderia sp. 1-acenaphthenol + NAD+ + ?
-
 ?
indan + NADH + H+ + O2
-
 Burkholderia sp. 1-indanol + 1-indanone + indene + NAD+
-
 ?
indan + NADH + H+ + O2
-
 Burkholderia sp. 1-indanol + ? + NAD+ no formation of 1,2-dihydroxyindan ?
indene + NADH + H+ + O2
-
 Burkholderia sp. 1-indenol + 1,2-dihydroxyindan + NAD+
-
 ?
indole + NADH + H+ + O2
-
 Burkholderia sp. indigo + NAD+ + ?
-
 ?
indoline + NADH + H+ + O2
-
 Burkholderia sp. indigo + ? + NAD+
-
 ?
indoline + NADH + H+ + O2
-
 Burkholderia sp. indole + NAD+ + ?
-
 ?
additional information no activity with anisole, carbazole, and nitrobenzene Burkholderia sp. ?
-
 ?
naphthalene + NADH + H+ + O2
-
 Burkholderia sp. (+)-cis-(1R,2S)-dihydroxy-1,2-dihydronaphthalene + NAD+
-
 ?
naphthalene + O2
-
 Burkholderia sp. cis-1,2-dihydroxy-1,2-dihydronaphthalene product is formed in 92% enantiomeric excess ?
phenetole + NADH + H+ + O2
-
 Burkholderia sp. phenol + NAD+ + ?
-
 ?

Synonyms

Synonyms Comment Organism
DNT dioxygenase
-
 Burkholderia sp.
dntA
-
 Burkholderia sp.

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Burkholderia sp.

General Information

General Information Comment Organism
physiological function an Escherichia coli clone that expresses DNT dioxygenase converts dinitrotoluene to 4-methyl-5-nitrocatechol and naphthalene to (+)-cis-(1R,2S)-dihydroxy-1,2-dihydronaphthalene. The enzyme system exhibits similar substrate specificities as naphthalene dioxygenase, both can oxidize such compounds as indole, indan, indene, phenetole, and acenaphthene Burkholderia sp.