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Literature summary for 1.14.12.23 extracted from

  • Li, T.; Gao, Y.Z.; Xu, J.; Zhang, S.T.; Guo, Y.; Spain, J.C.; Zhou, N.Y.
    A Recently assembled degradation pathway for 2,3-dichloronitrobenzene in Diaphorobacter sp. Strain JS3051 (2021), mBio, 12, e0223121.
    View publication on PubMed
Search for more literature for 1.14.12.23

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Diaphorobacter sp. JS3051

Protein Variants

Protein Variants Comment Organism
E204I the mutation causes a shift in preference from meta- to ortho-substituted substrates, such as 2-chloronitrobenzene, 2-nitrotoluene, and 2,3-dichloronitrobenzene. Unexpectedly, 2,3-dichloronitrobenzene is still a primary substrate of the variant Diaphorobacter sp. JS3051

Organism

Organism UniProt Comment Textmining
Diaphorobacter sp. JS3051
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,3-dichloronitrobenzene + NADH + O2 highest activity Diaphorobacter sp. JS3051 3,4-dichlorocatechol + nitrite + NAD+
-
 ?
2-dichloronitrobenzene + NADH + O2 low activity Diaphorobacter sp. JS3051 ? + nitrite + NAD+
-
 ?
2-nitrotoluene + NADH + O2 low activity Diaphorobacter sp. JS3051 ? + nitrite + NAD+
-
 ?
3,4-dichloronitrobenzene + NADH + O2 low activity Diaphorobacter sp. JS3051 ? + nitrite + NAD+
-
 ?
3-chloronitrobenzene + NADH + O2 second highest activity Diaphorobacter sp. JS3051 ? + nitrite + NAD+
-
 ?
3-nitrotoluene + NADH + O2 third highest activity Diaphorobacter sp. JS3051 ? + nitrite + NAD+
-
 ?
additional information no activity with 2,4-dichloronitrobenzene, 4-nitrotoluene, 4-dichloronitrobenzene, and nitrobenzene Diaphorobacter sp. JS3051 ?
-
 ?

Synonyms

Synonyms Comment Organism
2,3-dichloronitrobenzene dioxygenase
-
 Diaphorobacter sp. JS3051
23DCNB dioxygenase
-
 Diaphorobacter sp. JS3051
23DCNBDO
-
 Diaphorobacter sp. JS3051
DcbAaAbAcAd
-
 Diaphorobacter sp. JS3051

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Diaphorobacter sp. JS3051