BRENDA - Enzyme Database show
show all sequences of 1.14.12.23

Patchwork assembly of nag-like nitroarene dioxygenase genes and the 3-chlorocatechol degradation cluster for evolution of the 2-chloronitrobenzene catabolism pathway in Pseudomonas stutzeri ZWLR2-1

Liu, H.; Wang, S.J.; Zhang, J.J.; Dai, H.; Tang, H.; Zhou, N.Y.; Appl. Environ. Microbiol. 77, 4547-4552 (2011)

Data extracted from this reference:

Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas stutzeri ZWLR2-1
F4Y9I5 and F4Y9I4 and F4Y9J0 and F4Y9J1
F4Y9I5 i.e. ferredoxin reductase cnbAa, F4Y9I4 i.e. ferredoxin cnbAb, F4Y9J0 i.e. 2-chloronitrobenzene dioxygenase alpha subunit cnbAc, F4Y9J1 i.e. 2-chloronitrobenzene dioxygenase beta subunit cnbAd
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-chloronitrobenzene + NADH + O2
-
733159
Pseudomonas stutzeri ZWLR2-1
3-chlorocatechol + nitrite + NAD+
-
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-chloronitrobenzene + NADH + O2
-
733159
Pseudomonas stutzeri ZWLR2-1
3-chlorocatechol + nitrite + NAD+
-
-
-
?
General Information
General Information
Commentary
Organism
physiological function
Escherichia coli cells expressing dioxygenase genes cnbAaAbAcAd convert 2-chloronitrobenzene to 3-chlorocatechol with concomitant nitrite release. The cnbCDEF gene cluster, homologous to a 3-chlorocatechol degradation cluster in Sphingomonas sp. strain TFD44, probably contains all of the genes necessary for the conversion of 3-chlorocatechol to 3-oxoadipate
Pseudomonas stutzeri ZWLR2-1
General Information (protein specific)
General Information
Commentary
Organism
physiological function
Escherichia coli cells expressing dioxygenase genes cnbAaAbAcAd convert 2-chloronitrobenzene to 3-chlorocatechol with concomitant nitrite release. The cnbCDEF gene cluster, homologous to a 3-chlorocatechol degradation cluster in Sphingomonas sp. strain TFD44, probably contains all of the genes necessary for the conversion of 3-chlorocatechol to 3-oxoadipate
Pseudomonas stutzeri ZWLR2-1
Other publictions for EC 1.14.12.23
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
733176
Mahan
Selection for growth on 3-nitr ...
Acidovorax sp., Acidovorax sp. JS42
Appl. Environ. Microbiol.
81
309-319
2015
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4
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18
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17
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1
1
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18
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733264
Singh
Expression, purification and s ...
Diaphorobacter sp. DS2
Biochem. Biophys. Res. Commun.
445
36-42
2014
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1
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733159
Liu
Patchwork assembly of nag-like ...
Pseudomonas stutzeri ZWLR2-1
Appl. Environ. Microbiol.
77
4547-4552
2011
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733154
Ju
Control of substrate specifici ...
Comamonas sp.
Appl. Environ. Microbiol.
72
1817-1824
2006
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1
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6
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10
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733153
Parales
Purification, characterization ...
Acidovorax sp., Acidovorax sp. JS42
Appl. Environ. Microbiol.
71
3806-3814
2005
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1
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2
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7
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3
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734066
Keenan
Protein engineering of the arc ...
Ralstonia sp., Ralstonia sp. U2
J. Bacteriol.
187
3302-3310
2005
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4
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6
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734469
Friemann
Structural insight into the di ...
Comamonas sp., Comamonas sp. JS7650
J. Mol. Biol.
348
1139-1151
2005
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1
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733150
Lessner
Molecular characterization and ...
Comamonas sp.
Appl. Environ. Microbiol.
68
634-641
2002
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734057
Parales
Enzyme specificity of 2-nitrot ...
Pseudomonas sp., Pseudomonas sp. JS42
J. Bacteriol.
180
1194-1199
1998
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