Literature summary for 1.14.12.18 extracted from

Ohmori, T.; Morita, H.; Tanaka, M.; Tomoi, M.; Miyauchi, K.; Kasai, D.; Furukawa, K.; Masai, E.; Fukuda, M.
Expression in Escherichia coli of biphenyl 2,3-dioxygenase genes from a Gram-positive polychlorinated biphenyl degrader, Rhodococcus jostii RHA1 (2011), Biosci. Biotechnol. Biochem., 75, 26-33.

Search for more literature for 1.14.12.18

Cloned(Commentary)

Cloned (Comment)	Organism
gene cluster bphAaAbAcAd, genetic organization, the multicomponent enzyme components cannot be expressed in Escherichia coli actively due to lack of activity of the ferredoxin component in Escherichia coli involving codon usage bias. Therefore hybrid BphA gene derivatives are constructed by replacing ferredoxin and/or reductase components of RHA1 with those of Pseudomonas pseudoalcaligenes strain KF707. Expression of ferredoxin encoding gene bphAc in Escherichia coli strain Rosetta (DE3) pLacI resulting in an active protein	Rhodococcus jostii

Cloned (Comment)

Organism

gene cluster bphAaAbAcAd, genetic organization, the multicomponent enzyme components cannot be expressed in Escherichia coli actively due to lack of activity of the ferredoxin component in Escherichia coli involving codon usage bias. Therefore hybrid BphA gene derivatives are constructed by replacing ferredoxin and/or reductase components of RHA1 with those of Pseudomonas pseudoalcaligenes strain KF707. Expression of ferredoxin encoding gene bphAc in Escherichia coli strain Rosetta (DE3) pLacI resulting in an active protein

Rhodococcus jostii

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Fe2+	one Fe+ and one [Fe2-S2] cluster in the large subunit, as well as one [Fe2-S2] cluster in the small subunit of the enzyme complex	Rhodococcus jostii

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
biphenyl + NADH + H+ + O2	Rhodococcus jostii	-	(1S,2R)-3-phenylcyclohexa-3,5-diene-1,2-diol + NAD+	-	?

Organism

Organism	UniProt	Comment	Textmining
Rhodococcus jostii	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
biphenyl + NADH + H+ + O2	-	Rhodococcus jostii	(1S,2R)-3-phenylcyclohexa-3,5-diene-1,2-diol + NAD+	-	?

Synonyms

Synonyms	Comment	Organism
BphA	-	Rhodococcus jostii

Cofactor

Cofactor	Comment	Organism	Structure
FAD	in the reductase component of the enzyme complex	Rhodococcus jostii
NADH	-	Rhodococcus jostii

General Information

General Information	Comment	Organism
additional information	the multicomponent enzyme consists of small and large subunits of the oxygenase component, and ferredoxin and reductase components	Rhodococcus jostii