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Literature summary for 1.14.12.18 extracted from

  • Kumar, P.; Mohammadi, M.; Viger, J.F.; Barriault, D.; Gomez-Gil, L.; Eltis, L.D.; Bolin, J.T.; Sylvestre, M.
    Structural insight into the expanded PCB-degrading abilities of a biphenyl dioxygenase obtained by directed evolution (2011), J. Mol. Biol., 405, 531-547.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.12.18

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli DH11S cells Paraburkholderia xenovorans

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, using 20–25% (w/v) PEG 8000 or PEG 5000 MME, 50 mM PIPES (pH 6.5), 100 mM ammonium acetate, 5% (v/v) glycerol, and 0.2% (w/v) agarose Paraburkholderia xenovorans

Protein Variants

Protein Variants Comment Organism
F336M the mutant produces principally 3,4-dihydro-3,4-dihydroxy-2,2'-dichlorobiphenyl from 2,2'-dichlorobiphenyl Paraburkholderia xenovorans
T335A the mutant yields 2,3-dihydroxy-2,2'chlorobiphenyl as the major metabolite from 2,2'-dichlorobiphenyl Paraburkholderia xenovorans
T335A/F336M the mutant metabolizes 2,2'-dichlorobiphenyl better than the wild type enzyme to generate principally 3,4-dihydro-3,4-dihydroxy-2,2'-dichlorobiphenyl instead of 2,3-dihydroxy-2'-chlorobiphenyl as produced by the wild type enzyme, the mutant produces 3',4'-dihydroxy-3',4'-dihydro-2,6-dichlorobiphenyl as a major metabolite from 2,6-dichlorobiphenyl Paraburkholderia xenovorans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
biphenyl + NADH + H+ + O2 Paraburkholderia xenovorans
-
 cis-(2R,3S)-dihydroxy-1-phenylcyclohexa-4,6-diene + NAD+
-
 ?

Organism

Organism UniProt Comment Textmining
Paraburkholderia xenovorans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Paraburkholderia xenovorans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-dichlorobiphenyl + NADH + H+ + O2 poor substrate Paraburkholderia xenovorans 2,3-dihydroxy-2'-chlorobiphenyl + NAD+ + HCl
-
 ?
2,6-dichlorobiphenyl + NADH + H+ + O2 poor substrate Paraburkholderia xenovorans ?
-
 ?
biphenyl + NADH + H+ + O2
-
 Paraburkholderia xenovorans cis-(2R,3S)-dihydroxy-1-phenylcyclohexa-4,6-diene + NAD+
-
 ?

Synonyms

Synonyms Comment Organism
BPDO
-
 Paraburkholderia xenovorans

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Paraburkholderia xenovorans

General Information

General Information Comment Organism
metabolism BPDO is one of the most potent biocatalysts of natural origin for the dioxygenation of chlorobiphenyls Paraburkholderia xenovorans