Cloned (Comment) | Organism |
---|---|
tobacco plants transiently expressing BPDO | Paraburkholderia xenovorans LB400 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paraburkholderia xenovorans LB400 | - |
- |
- |
Rhodococcus jostii RHA1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
active BphAE and BphG copurified from Nicotiana benthamiana leaves | Paraburkholderia xenovorans LB400 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Phe378 and Phe384 are near the substrate inside the catalytic pocket and thus likely interact with it | Rhodococcus jostii RHA1 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Biphenyl 2,3-dioxygenase | - |
Rhodococcus jostii RHA1 |
Biphenyl 2,3-dioxygenase | - |
Paraburkholderia xenovorans LB400 |
biphenyl dioxygenase | - |
Rhodococcus jostii RHA1 |
biphenyl dioxygenase | - |
Paraburkholderia xenovorans LB400 |
BPDO | - |
Rhodococcus jostii RHA1 |
BPDO | - |
Paraburkholderia xenovorans LB400 |
BphA1A2 | - |
Rhodococcus jostii RHA1 |
BphAE | - |
Paraburkholderia xenovorans LB400 |
General Information | Comment | Organism |
---|---|---|
physiological function | catalyzes critical steps of the bacterial polychlorinated biphenyl degrading pathway | Rhodococcus jostii RHA1 |
physiological function | catalyzes critical steps of the bacterial polychlorinated biphenyl degrading pathway. Transgenic plants cotransformed with pGreenbphA + bphE + bphG + pGreen-bphF do not produce the three enzyme components simultaneously. Frequency of viable plants among transformants is low, thus simultaneous expression of all four BPDO genes in transgenic tobacco is hampered by genetic or physiological reasons | Paraburkholderia xenovorans LB400 |