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Literature summary for 1.14.12.18 extracted from

  • Vezina, J.; Barriault, D.; Sylvestre, M.
    Family shuffling of soil DNA to change the regiospecificity of Burkholderia xenovorans LB400 biphenyl dioxygenase (2007), J. Bacteriol., 189, 779-788.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.12.18

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Paraburkholderia xenovorans

Protein Variants

Protein Variants Comment Organism
F370Y lower reactivity toward 2,2-dichlorobiphenyl but unchanged regiospecificity toward this substrate compared to the wild type enzyme Paraburkholderia xenovorans
L283S lower reactivity toward 2,2-dichlorobiphenyl but unchanged regiospecificity toward this substrate compared to the wild type enzyme Paraburkholderia xenovorans
M237T lower reactivity toward 2,2-dichlorobiphenyl but unchanged regiospecificity toward this substrate compared to the wild type enzyme Paraburkholderia xenovorans
S238T lower reactivity toward 2,2-dichlorobiphenyl but unchanged regiospecificity toward this substrate compared to the wild type enzyme Paraburkholderia xenovorans
T377N lower reactivity toward 2,2-dichlorobiphenyl but unchanged regiospecificity toward this substrate compared to the wild type enzyme Paraburkholderia xenovorans

Organism

Organism UniProt Comment Textmining
Paraburkholderia xenovorans
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2',3,3'-tetrachlorobiphenyl + NADH + H+ + O2
-
 Paraburkholderia xenovorans 5,6-dihydro-5,6-dihydroxy-2,2',3,3'-tetrachlorobiphenyl + 4,5-dihydro-4,5-dihydroxy-2,2',3,3'-tetrachlorobiphenyl + NAD+
-
 ?
2,2',5,5'-tetrachlorobiphenyl + NADH + H+ + O2
-
 Paraburkholderia xenovorans 3,4-dihydro-3,4-dihydroxy-2,2',5,5'-tetrachlorobiphenyl + NAD+
-
 ?
2,2'-dichlorobiphenyl + NADH + H+ + O2
-
 Paraburkholderia xenovorans 2,3-dihydroxy-2'-chlorobiphenyl + 3,4-dihydro-3,4-dihydroxy-2,2'-dichlorobiphenyl + NAD+ + HCl
-
 ?
2,4',5-trichlorobiphenyl + NADH + H+ + O2
-
 Paraburkholderia xenovorans 3,4-dihydro-3,4-dihydroxy-2,5,4'-trichlorobiphenyl + 2',3'-dihydro-2',3'-dihydroxy-2,5,4'-trichlorobiphenyl + NAD+
-
 ?
biphenyl + NAD(P)H + O2
-
 Paraburkholderia xenovorans cis-biphenyl 2,3-dihydrodiol + NAD(P)+
-
 ?
ethylbenzene + NADH + O2
-
 Paraburkholderia xenovorans ?
-
 ?
additional information no activity on toluene and benzene Paraburkholderia xenovorans ?
-
 ?

Synonyms

Synonyms Comment Organism
biphenyl dioxygenase
-
 Paraburkholderia xenovorans
BPDO
-
 Paraburkholderia xenovorans
BphAE
-
 Paraburkholderia xenovorans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Paraburkholderia xenovorans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Paraburkholderia xenovorans

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Paraburkholderia xenovorans
NADPH
-
 Paraburkholderia xenovorans