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Literature summary for 1.14.12.18 extracted from
- Steady-state kinetic characterization of evolved biphenyl dioxygenase, which acquired novel degradation ability for benzene and toluene (2006), Biosci. Biotechnol. Biochem., 70, 1021-1025.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Pseudomonas oleovorans |
| expressed in Escherichia coli | Paraburkholderia xenovorans |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0027 | - |
biphenyl | Pseudomonas pseudoalcaligenes strain 1072 | Pseudomonas oleovorans |  |
| 0.00342 | - |
biphenyl | Pseudomonas pseudoalcaligenes strain KF707 | Pseudomonas oleovorans | |
| 0.00368 | - |
biphenyl | Pseudomonas pseudoalcaligenes strain LB400 | Pseudomonas oleovorans | |
| 0.00433 | - |
Benzene | Pseudomonas pseudoalcaligenes strain 1072 | Pseudomonas oleovorans | |
| 0.0108 | - |
ethylbenzene | Pseudomonas pseudoalcaligenes strain 1072 | Pseudomonas oleovorans | |
| 0.0117 | - |
Toluene | Pseudomonas pseudoalcaligenes strain 1072 | Pseudomonas oleovorans | |
| 0.0236 | - |
ethylbenzene | Pseudomonas pseudoalcaligenes strain KF707 | Pseudomonas oleovorans | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Paraburkholderia xenovorans | - |
- |
- |
| Pseudomonas oleovorans | - |
strains KF707 and 1072 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Q-Sepharose FF column chromatography | Pseudomonas oleovorans |
| Q-Sepharose FF column chromatography | Paraburkholderia xenovorans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| benzene + ? | benzene is only a substrate for Pseudomonas pseudoalcaligenes strain 1072 | Pseudomonas oleovorans | ? | - |
? | |
| biphenyl + NAD(P)H + O2 | - |
Pseudomonas oleovorans | cis-biphenyl 2,3-dihydrodiol + NAD(P)+ | - |
? | |
| biphenyl + NAD(P)H + O2 | - |
Paraburkholderia xenovorans | cis-biphenyl 2,3-dihydrodiol + NAD(P)+ | - |
? | |
| ethylbenzene + ? | - |
Pseudomonas oleovorans | ? | - |
? | |
| toluene + NAD(P)H + O2 | toluene is only a substrate for Pseudomonas pseudoalcaligenes strain 1072 | Pseudomonas oleovorans | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| biphenyl dioxygenase | - |
Pseudomonas oleovorans |
| biphenyl dioxygenase | - |
Paraburkholderia xenovorans |
| BPH dox | - |
Pseudomonas oleovorans |
| BPH dox | - |
Paraburkholderia xenovorans |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0497 | - |
ethylbenzene | Pseudomonas pseudoalcaligenes strain KF707 | Pseudomonas oleovorans | |
| 0.056 | - |
Benzene | Pseudomonas pseudoalcaligenes strain 1072 | Pseudomonas oleovorans | |
| 0.113 | - |
biphenyl | Pseudomonas pseudoalcaligenes strain KF707 | Pseudomonas oleovorans | |
| 0.114 | - |
biphenyl | Pseudomonas pseudoalcaligenes strain LB400 | Pseudomonas oleovorans | |
| 0.115 | - |
Toluene | Pseudomonas pseudoalcaligenes strain 1072 | Pseudomonas oleovorans | |
| 0.168 | - |
biphenyl | Pseudomonas pseudoalcaligenes strain 1072 | Pseudomonas oleovorans | |
| 0.219 | - |
ethylbenzene | Pseudomonas pseudoalcaligenes strain 1072 | Pseudomonas oleovorans | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | - |
Pseudomonas oleovorans | |
| NADH | - |
Paraburkholderia xenovorans | |
| NADPH | - |
Pseudomonas oleovorans | |
| NADPH | - |
Paraburkholderia xenovorans | |
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