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Literature summary for 1.14.12.18 extracted from

  • Suenaga, H.; Sato, M.; Goto, M.; Takeshita, M.; Furukawa, K.
    Steady-state kinetic characterization of evolved biphenyl dioxygenase, which acquired novel degradation ability for benzene and toluene (2006), Biosci. Biotechnol. Biochem., 70, 1021-1025.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Pseudomonas oleovorans
expressed in Escherichia coli Paraburkholderia xenovorans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0027
-
biphenyl Pseudomonas pseudoalcaligenes strain 1072 Pseudomonas oleovorans
0.00342
-
biphenyl Pseudomonas pseudoalcaligenes strain KF707 Pseudomonas oleovorans
0.00368
-
biphenyl Pseudomonas pseudoalcaligenes strain LB400 Pseudomonas oleovorans
0.00433
-
Benzene Pseudomonas pseudoalcaligenes strain 1072 Pseudomonas oleovorans
0.0108
-
ethylbenzene Pseudomonas pseudoalcaligenes strain 1072 Pseudomonas oleovorans
0.0117
-
Toluene Pseudomonas pseudoalcaligenes strain 1072 Pseudomonas oleovorans
0.0236
-
ethylbenzene Pseudomonas pseudoalcaligenes strain KF707 Pseudomonas oleovorans

Organism

Organism UniProt Comment Textmining
Paraburkholderia xenovorans
-
-
-
Pseudomonas oleovorans
-
strains KF707 and 1072
-

Purification (Commentary)

Purification (Comment) Organism
Q-Sepharose FF column chromatography Pseudomonas oleovorans
Q-Sepharose FF column chromatography Paraburkholderia xenovorans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzene + ? benzene is only a substrate for Pseudomonas pseudoalcaligenes strain 1072 Pseudomonas oleovorans ?
-
?
biphenyl + NAD(P)H + O2
-
Pseudomonas oleovorans cis-biphenyl 2,3-dihydrodiol + NAD(P)+
-
?
biphenyl + NAD(P)H + O2
-
Paraburkholderia xenovorans cis-biphenyl 2,3-dihydrodiol + NAD(P)+
-
?
ethylbenzene + ?
-
Pseudomonas oleovorans ?
-
?
toluene + NAD(P)H + O2 toluene is only a substrate for Pseudomonas pseudoalcaligenes strain 1072 Pseudomonas oleovorans ?
-
?

Synonyms

Synonyms Comment Organism
biphenyl dioxygenase
-
Pseudomonas oleovorans
biphenyl dioxygenase
-
Paraburkholderia xenovorans
BPH dox
-
Pseudomonas oleovorans
BPH dox
-
Paraburkholderia xenovorans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0497
-
ethylbenzene Pseudomonas pseudoalcaligenes strain KF707 Pseudomonas oleovorans
0.056
-
Benzene Pseudomonas pseudoalcaligenes strain 1072 Pseudomonas oleovorans
0.113
-
biphenyl Pseudomonas pseudoalcaligenes strain KF707 Pseudomonas oleovorans
0.114
-
biphenyl Pseudomonas pseudoalcaligenes strain LB400 Pseudomonas oleovorans
0.115
-
Toluene Pseudomonas pseudoalcaligenes strain 1072 Pseudomonas oleovorans
0.168
-
biphenyl Pseudomonas pseudoalcaligenes strain 1072 Pseudomonas oleovorans
0.219
-
ethylbenzene Pseudomonas pseudoalcaligenes strain 1072 Pseudomonas oleovorans

Cofactor

Cofactor Comment Organism Structure
NADH
-
Pseudomonas oleovorans
NADH
-
Paraburkholderia xenovorans
NADPH
-
Pseudomonas oleovorans
NADPH
-
Paraburkholderia xenovorans