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Literature summary for 1.14.12.18 extracted from

  • Suenaga, H.; Goto, M.; Furukawa, K.
    Active-site engineering of biphenyl dioxygenase: effect of substituted amino acids on substrate specificity and regiospecificity (2006), Appl. Microbiol. Biotechnol., 71, 168-176.
    View publication on PubMed
Search for more literature for 1.14.12.18

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Pseudomonas oleovorans

Protein Variants

Protein Variants Comment Organism
I335F/T376N does not show any significant difference in the oxidation of biphenyl compared with wild type Bph Dox, and exhibits 2,3-dioxygenase activity for 2,2'-dichlorobiphenyl and 3,4-dioxygenase activity for 2,5,4'-trichlorobiphenyl Pseudomonas oleovorans
T376D decreased degradation activity Pseudomonas oleovorans
T376F shows novel degradation activity for dibenzofuran, which is a poor substrate for the wild type enzyme Pseudomonas oleovorans
T376G decreased degradation activity Pseudomonas oleovorans
T376K shows novel degradation activity for dibenzofuran, which is a poor substrate for the wild type enzyme Pseudomonas oleovorans
T376N shows novel degradation activity for dibenzofuran, which is a poor substrate for the wild type enzyme Pseudomonas oleovorans
T376S decreased degradation activity Pseudomonas oleovorans
T376V shows novel degradation activity for dibenzofuran, which is a poor substrate for the wild type enzyme Pseudomonas oleovorans

Organism

Organism UniProt Comment Textmining
Pseudomonas oleovorans
-
-
-
Pseudomonas oleovorans KF707
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-dichlorobiphenyl + NADH + O2
-
 Pseudomonas oleovorans 2,3-dihydroxy-2'-chlorobiphenyl + 3,4-dihydro-3,4-dihydroxy-2,2'-dichlorobiphenyl + NAD+
-
 ?
2,2'-dichlorobiphenyl + NADH + O2
-
 Pseudomonas oleovorans KF707 2,3-dihydroxy-2'-chlorobiphenyl + 3,4-dihydro-3,4-dihydroxy-2,2'-dichlorobiphenyl + NAD+
-
 ?
2,5,4'-trichlorobiphenyl + NADH + O2
-
 Pseudomonas oleovorans ?
-
 ?
2,5,4'-trichlorobiphenyl + NADH + O2
-
 Pseudomonas oleovorans KF707 ?
-
 ?
4,4'-dichlorobiphenyl + NAD(P)H + O2
-
 Pseudomonas oleovorans 2,3-dihydroxy-4,4'-dichlorobiphenyl + NAD(P)+
-
 ?
4,4'-dichlorobiphenyl + NAD(P)H + O2
-
 Pseudomonas oleovorans KF707 2,3-dihydroxy-4,4'-dichlorobiphenyl + NAD(P)+
-
 ?
4-methylbiphenyl + NADH + O2
-
 Pseudomonas oleovorans 2-hydroxy-6-oxo-6-[4-methylphenyl]-hexa-2,4-dienoic acid + NAD+
-
 ?
4-methylbiphenyl + NADH + O2
-
 Pseudomonas oleovorans KF707 2-hydroxy-6-oxo-6-[4-methylphenyl]-hexa-2,4-dienoic acid + NAD+
-
 ?
biphenyl + NAD(P)H + O2
-
 Pseudomonas oleovorans cis-biphenyl 2,3-dihydrodiol + NAD(P)+
-
 ?
biphenyl + NAD(P)H + O2
-
 Pseudomonas oleovorans KF707 cis-biphenyl 2,3-dihydrodiol + NAD(P)+
-
 ?
dibenzofuran + NADH + O2 dibenzofuran is a poor substrate Pseudomonas oleovorans ?
-
 ?
diphenylmethane + NADH + O2
-
 Pseudomonas oleovorans ?
-
 ?

Synonyms

Synonyms Comment Organism
biphenyl dioxygenase
-
 Pseudomonas oleovorans
BPH dox
-
 Pseudomonas oleovorans
BphABC
-
 Pseudomonas oleovorans

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Pseudomonas oleovorans
NADPH
-
 Pseudomonas oleovorans