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Literature summary for 1.14.12.18 extracted from

  • Barriault, D.; Simard, C.; Chatel, H.; Sylvestre, M.
    Characterization of hybrid biphenyl dioxygenases obtained by recombining Burkholderia sp. strain LB400 bphA with the homologous gene of Comamonas testosteroni B-356 (2001), Can. J. Microbiol., 47, 1025-1032.
    View publication on PubMed
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Protein Variants

Protein Variants Comment Organism
additional information characterization of hybrid biphenyl dioxygenases obtained by recombining Burkholderia sp. strain Comamonas testosteroni LB400 bphA with the homologous gene of Comamonas testosteroni B-356. The C-terminal portion of Burkholderia sp. LB400 alpha-subunit can withstand extensive structural modifications, and that these modifications can change the catalytic properties of the enzyme. Exchanging the C-terminal portion of Comamonas testosteroni B-356 BPDO alpha-subunit with that of Burkholderia sp. LB400 alpha-subunit generates inactive chimeras Comamonas testosteroni
additional information characterization of hybrid biphenyl dioxygenases obtained by recombining Burkholderia sp. strain Comamonas testosteroni LB400 bphA with the homologous gene of Comamonas testosteroni B-356. The C-terminal portion of Burkholderia sp. LB400 alpha-subunit can withstand extensive structural modifications, and that these modifications can change the catalytic properties of the enzyme. Exchanging the C-terminal portion of Comamonas testosteroni B-356 BPDO alpha-subunit with that of Burkholderia sp. LB400 alpha-subunit generates inactive chimeras Burkholderia sp.

Organism

Organism UniProt Comment Textmining
Burkholderia sp.
-
 strain LB400, hybrid biphenyl dioxygenases obtained by recombining Burkholderia sp. strain LB400 bphA with the homologous gene of Comamonas testosteroni B-356
-
Comamonas testosteroni
-
 strain B-356, hybrid biphenyl dioxygenases obtained by recombining Burkholderia sp. strain LB400 bphA with the homologous gene of Comamonas testosteroni B-356
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2',5,5'-tetrachlorobiphenyl + NADH + H+ + O2
-
 Burkholderia sp. ?
-
 ?
2,2'-dichlorobiphenyl + NADH + H+ + O2
-
 Burkholderia sp. ?
-
 ?
2,2'-dichlorobiphenyl + NADH + H+ + O2 wild-type enzyme Comamonas testosteroni ?
-
 ?
3,3'-dichlorobiphenyl + NADH + H+ + O2 wild-type enzyme Comamonas testosteroni ?
-
 ?
4,4'-dichlorobiphenyl + NADH + H+ + O2
-
 Burkholderia sp. ?
-
 ?
biphenyl + NADH + H+ + O2
-
 Burkholderia sp. ?
-
 ?
biphenyl + NADH + H+ + O2 wild-type enzyme Comamonas testosteroni ?
-
 ?
additional information no activity with 3,3'-dichlorobiphenyl Burkholderia sp. ?
-
 ?
additional information no activity with 4,4'-dichlorobiphenyl and 2,2',5,5'-tetrachlorobiphenyl Comamonas testosteroni ?
-
 ?

Synonyms

Synonyms Comment Organism
BPDO
-
 Comamonas testosteroni
BPDO
-
 Burkholderia sp.