Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | omission of ferrous ammonium sulfate reduces specific activity of purified ISP 7.4fold | Pseudomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27300 | - |
beta subunit of ISP, SDS-PAGE | Pseudomonas sp. |
53000 | - |
alpha subunit of ISP, SDS-PAGE | Pseudomonas sp. |
209000 | - |
native protein, gel filtration with Superose 12 | Pseudomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
- |
- |
Pseudomonas sp. LB400 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
purification of the iron sulfur protein (ISP) of biphenyl 2,3-dioxygenase | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
biphenyl + NAD(P)H + O2 | - |
Pseudomonas sp. | cis-biphenyl 2,3-dihydrodiol + NAD(P)+ | - |
? | |
biphenyl + NAD(P)H + O2 | - |
Pseudomonas sp. LB400 | cis-biphenyl 2,3-dihydrodiol + NAD(P)+ | - |
? | |
carbazole + NADH + O2 | no products with carbazole detected | Pseudomonas sp. | 3-hydroxycarbazole + NAD+ | - |
? | |
carbazole + NADH + O2 | no products with carbazole detected | Pseudomonas sp. LB400 | 3-hydroxycarbazole + NAD+ | - |
? | |
additional information | no substrates are benzene, toluene, 2,5-dichlorotoluene, carbazole and dibenzothiophene | Pseudomonas sp. | ? | - |
? | |
additional information | no substrates are benzene, toluene, 2,5-dichlorotoluene, carbazole and dibenzothiophene | Pseudomonas sp. LB400 | ? | - |
? | |
naphthalene + NADPH + O2 | poor substrate | Pseudomonas sp. | cis-naphthalene 1,2-dihydrodiol + NADP+ | - |
? | |
naphthalene + NADPH + O2 | poor substrate | Pseudomonas sp. LB400 | cis-naphthalene 1,2-dihydrodiol + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterohexamer | alpha3,beta3, 3 * 53000 + 3 * 27300, measurement of molecular weight and Stokes' radius with gel filtration | Pseudomonas sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
Ferredoxin | - |
Pseudomonas sp. |