Literature summary for 1.14.12.11 extracted from

Jiang, H.; Parales, R.E.; Lynch, N.A.; Gibson, D.T.
Site-directed mutagenesis of conserved amino acids in the alpha subunit of toluene dioxygenase: potential mononuclear non-heme iron coordination sites (1996), J. Bacteriol., 178, 3133-3139.

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Protein Variants

Protein Variants	Comment	Organism
D219A	mutation at alpha-subunit of oxygenase component completely abolishes toluene dioxygenase activity, mutation completely eliminates formation of cis-toluene dihydrodiol	Pseudomonas putida
E214A	mutation at alpha-subunit of oxygenase component completely abolishes toluene dioxygenase activity, mutation completely eliminates formation of cis-toluene dihydrodiol	Pseudomonas putida
H222A	mutation at alpha-subunit of oxygenase component completely abolishes toluene dioxygenase activity, mutation completely eliminates formation of cis-toluene dihydrodiol	Pseudomonas putida
H228A	mutation at alpha-subunit of oxygenase component completely abolishes toluene dioxygenase activity, mutation completely eliminates formation of cis-toluene dihydrodiol	Pseudomonas putida
Y221A	mutation at alpha-subunit of oxygenase component, 42% of the activity of the wild-type enzyme, formation of cis-toluene dihydrodiol is reduced	Pseudomonas putida
Y266A	mutation at alpha-subunit of oxygenase component, 12% of the activity of the wild-type enzyme, formation of cis-toluene dihydrodiol is reduced	Pseudomonas putida

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Iron	enzyme requires mononuclear iron for activity	Pseudomonas putida

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas putida	-	F1	-

Purification (Commentary)

Purification (Comment)	Organism
beta-subunit of oxygenase component	Pseudomonas putida

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
toluene + NADH + O2	-	Pseudomonas putida	(1S,2R)-3-methylcyclohexa-3,5-diene-1,2-diol + NAD+	-	?

Cofactor

Cofactor	Comment	Organism	Structure
NADH	-	Pseudomonas putida

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Release 2023.1 (January 2023)