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Literature summary for 1.14.12.11 extracted from

  • Zylstra, G.J.; Gibson, D.T.
    Toluene degradation by Pseudomonas putida F1. Nucleotide sequence of the todC1C2BADE genes and their expression in Escherichia coli (1989), J. Biol. Chem., 264, 14940-14946.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
genes encoding the three components of the toluene dioxygenase overproduced in Escherichia coli JM109: 1. the reductaseTOL - tolA, 2. the ferredoxinTOL and 3. the two subunits of the terminal dioxygenase - todC1C2 Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
F1
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
toluene + NADH + O2
-
Pseudomonas putida (1S,2R)-3-methylcyclohexa-3,5-diene-1,2-diol + NAD+
-
?

Cofactor

Cofactor Comment Organism Structure
NADH
-
Pseudomonas putida