BRENDA - Enzyme Database
show all sequences of 1.14.12.10

Rate-determining attack on substrate precedes rieske cluster oxidation during cis-dihydroxylation by benzoate dioxygenase

Rivard, B.S.; Rogers, M.S.; Marell, D.J.; Neibergall, M.B.; Chakrabarty, S.; Cramer, C.J.; Lipscomb, J.D.; Biochemistry 54, 4652-4664 (2015)

Data extracted from this reference:

Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
benzoate + NADH + H+ + O2
Pseudomonas putida
-
(1R,6S)-1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate + NAD+
-
-
?
benzoate + NADH + H+ + O2
Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182
-
(1R,6S)-1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate + NAD+
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas putida
-
-
-
Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
benzoate + NADH + H+ + O2
-
741916
Pseudomonas putida
(1R,6S)-1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate + NAD+
-
-
-
?
benzoate + NADH + H+ + O2
-
741916
Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182
(1R,6S)-1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate + NAD+
-
-
-
?
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
4.4
-
Benzoate
at pH 6.8 temperature not specified in the publication
Pseudomonas putida
Cofactor
Cofactor
Commentary
Organism
Structure
NADH
-
Pseudomonas putida
[2Fe-2S]-center
-
Pseudomonas putida
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADH
-
Pseudomonas putida
[2Fe-2S]-center
-
Pseudomonas putida
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
benzoate + NADH + H+ + O2
Pseudomonas putida
-
(1R,6S)-1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate + NAD+
-
-
?
benzoate + NADH + H+ + O2
Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182
-
(1R,6S)-1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate + NAD+
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
benzoate + NADH + H+ + O2
-
741916
Pseudomonas putida
(1R,6S)-1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate + NAD+
-
-
-
?
benzoate + NADH + H+ + O2
-
741916
Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182
(1R,6S)-1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate + NAD+
-
-
-
?
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
4.4
-
Benzoate
at pH 6.8 temperature not specified in the publication
Pseudomonas putida
Other publictions for EC 1.14.12.10
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
742546
Solyanikova
Kinetics of interaction betwe ...
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355-362
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2
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9
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1
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1
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3
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743275
Tavakoli
Expression, purification and ...
Rhodococcus ruber, Rhodococcus ruber UKMP-5M
Mol. Biol. Res. Commun.
5
133-142
2016
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1
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1
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2
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1
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8
1
1
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1
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8
1
1
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1
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1
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741649
Karandikar
Biochemical characterization ...
Pseudomonas aeruginosa, Pseudomonas aeruginosa PP4
Appl. Biochem. Biotechnol.
177
318-333
2015
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2
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741916
Rivard
Rate-determining attack on su ...
Pseudomonas putida, Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182
Biochemistry
54
4652-4664
2015
-
-
-
-
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2
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20
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2
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1
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2
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2
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-
2
-
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-
1
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686803
Kim
Molecular cloning and function ...
Chromohalobacter sp., Chromohalobacter sp. HS-2
FEMS Microbiol. Lett.
280
235-241
2008
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-
1
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2
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5
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2
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1
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2
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2
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-
-
-
-
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690587
Sun
Microbial production of cis-1, ...
Pseudomonas putida, Pseudomonas putida KT 2240
Appl. Microbiol. Biotechnol.
80
977-984
2008
-
1
1
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-
1
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4
-
46
-
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1
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4
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1
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1
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1
1
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1
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4
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1
-
4
-
1
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-
1
-
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-
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685176
Neibergall
Hydrogen peroxide dependent ci ...
Pseudomonas putida, Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182
Biochemistry
46
8004-8016
2007
-
-
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19
-
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2
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2
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677068
Kim
Analysis of aromatic catabolic ...
Pseudomonas putida, Pseudomonas putida KT 2440
Proteomics
6
1301-1318
2006
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-
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1
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5
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1
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2
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1
-
1
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1
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1
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1
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-
2
-
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-
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1
-
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658491
Xu
Genetic organization of genes ...
Acinetobacter calcoaceticus, Acinetobacter calcoaceticus PHEA-2
Curr. Microbiol.
46
235-240
2003
-
-
-
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1
2
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6
-
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2
1
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1
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1
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1
2
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2
1
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659031
Ge
Characterization of hybrid tol ...
Acinetobacter calcoaceticus, Acinetobacter calcoaceticus BADO ADP1
J. Bacteriol.
185
5333-5341
2003
-
-
1
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4
-
1
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2
-
4
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1
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1
1
10
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4
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2
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1
2
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4
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1
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2
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1
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1
1
10
-
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4
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396142
Wolfe
Benzoate 1,2-dioxygenase from ...
Pseudomonas putida, Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182
Biochemistry
41
9611-9626
2002
-
-
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1
5
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22
-
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1
1
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2
-
8
2
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3
-
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-
3
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1
5
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1
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2
-
8
2
-
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396141
Haddad
Cloning and expression of the ...
Rhodococcus sp., Rhodococcus sp. 19070
Appl. Environ. Microbiol.
67
2507-2514
2001
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-
1
-
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1
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6
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6
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1
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1
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6
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396140
Bundy
Similarities between the antAB ...
Acinetobacter sp.
J. Bacteriol.
180
4466-4474
1998
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1
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5
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1
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1
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1
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660529
Crosby
-
Synthesis of optically active ...
Pseudomonas putida
Tetrahedron
47
4789-4846
1991
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1
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1
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1
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396139
Yamaguchi
Subunit structure of oxygenase ...
Pseudomonas putida, Pseudomonas putida C-1
J. Biol. Chem.
257
12497-12502
1982
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1
4
2
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7
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2
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2
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4
2
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396138
Yamaguchi
Purification and characterizat ...
Pseudomonas putida, Pseudomonas putida C-1
J. Biol. Chem.
255
5058-5063
1980
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4
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1
4
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8
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1
1
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1
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11
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1
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1
2
1
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2
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2
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4
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1
4
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1
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1
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11
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1
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1
2
1
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