Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.11.9 extracted from

  • Sun, Y.J.; He, J.M.; Kong, J.Q.
    Characterization of two flavonol synthases with iron-independent flavanone 3-hydroxylase activity from Ornithogalum caudatum Jacq (2019), BMC Plant Biol., 19, 195 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.11.9

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Transetta (DE3) Albuca bracteata

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03757
-
 (2S)-naringenin pH 6.0, 20°C Albuca bracteata
0.11
-
 (2S)-naringenin pH 6.0, 20°C Albuca bracteata

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ the flavanone 3beta-hydroxylase activity of OcFLS1 and OcFLS2 does not absolutely require iron. However, the presence of sufficient Fe2+ is conducive to successive catalysis of flavanones to flavonols Albuca bracteata

Organism

Organism UniProt Comment Textmining
Albuca bracteata A0A482LTA5
-
-
Albuca bracteata A0A482LVU5
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Albuca bracteata

Source Tissue

Source Tissue Comment Organism Textmining
bulb
-
 Albuca bracteata
-
flower
-
 Albuca bracteata
-
leaf high expression Albuca bracteata
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2S)-naringenin + 2-oxoglutarate + O2
-
 Albuca bracteata (2R,3R)-dihydrokaempferol + succinate + CO2
-
 ?
(S)-eriodictyol + 2-oxoglutarate + O2
-
 Albuca bracteata dihydroquercetin + succinate + CO2
-
 ?
(S)-homoeriodictyol + 2-oxoglutarate + O2
-
 Albuca bracteata 3'-O-methyltaxifolin + succinate + CO2
-
 ?
(S)-pinocembrin + 2-oxoglutarate + O2
-
 Albuca bracteata pinobanksin + succinate + CO2
-
 ?

Synonyms

Synonyms Comment Organism
F3H
-
 Albuca bracteata
flavanone 3-hydroxylase
-
 Albuca bracteata
flavanone 3beta-hydroxylase
-
 Albuca bracteata
FLS1
-
 Albuca bracteata
FLS2
-
 Albuca bracteata

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20
-
-
 Albuca bracteata

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
-
 43 0°C: about 80% of maximal activity, 45°C: about 75% of maximal activity Albuca bracteata
5 50 5°C: about 90% of maximal activity, 50°C: about 50% of maximal activity Albuca bracteata

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Albuca bracteata

pH Range

pH Minimum pH Maximum Comment Organism
5 9 pH 5.0: about 40% of maximal activity, pH 9.0: about 50% of maximal activity Albuca bracteata
5 8 pH 5.0: about 75% of maximal activity, pH 8.0: about 40% of maximal activity Albuca bracteata