BRENDA - Enzyme Database
show all sequences of 1.14.11.9

Molecular cloning and identification of a flavanone 3-hydroxylase gene from Lycium chinense, and its overexpression enhances drought stress in tobacco

Song, X.; Diao, J.; Ji, J.; Wang, G.; Guan, C.; Jin, C.; Wang, Y.; Plant Physiol. Biochem. 98, 89-100 (2016)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
gene LcF3H, DNA and amino acid sequence determination and analysis, phylogenetic analysis, quantitative PCR enzyme expression analysis, recombinant expression in Escherichia coli strain BL21(DE3), recombinant overexpression in leaves of Nicotiana tabacum cv. Xanthinc via transformation with Agrobacterium tumefaciens strain EHA105, overexpression of heterogenous LcF3H enhances the expression levels of downstream genes but does not cause alteration in the expression of upstream genes including endogenous F3H of Nicotiana tabacum. The enzyme's overexpression does cause a significant increase in the content of three major flavan-3-ols, catechin, epicatechin and epigallocatechin
Lycium chinense
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
a (2S)-flavan-4-one + 2-oxoglutarate + O2
Lycium chinense
-
a (2R,3R)-dihydroflavonol + succinate + CO2
-
-
?
naringenin + 2-oxoglutarate + O2
Lycium chinense
-
dihydrokaempferol + succinate + CO2
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Lycium chinense
A0A068EM79
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
flower
-
Lycium chinense
-
leaf
primordial leaf, young leaf (highest level), and mature leaf
Lycium chinense
-
additional information
tissue pattern of enzyme expression, overview
Lycium chinense
-
root
low expression level
Lycium chinense
-
seedling
-
Lycium chinense
-
stem
-
Lycium chinense
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
a (2S)-flavan-4-one + 2-oxoglutarate + O2
-
746138
Lycium chinense
a (2R,3R)-dihydroflavonol + succinate + CO2
-
-
-
?
naringenin + 2-oxoglutarate + O2
-
746138
Lycium chinense
dihydrokaempferol + succinate + CO2
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
flavanone 3-hydroxylase
-
Lycium chinense
LcF3H
-
Lycium chinense
Cloned(Commentary) (protein specific)
Commentary
Organism
gene LcF3H, DNA and amino acid sequence determination and analysis, phylogenetic analysis, quantitative PCR enzyme expression analysis, recombinant expression in Escherichia coli strain BL21(DE3), recombinant overexpression in leaves of Nicotiana tabacum cv. Xanthinc via transformation with Agrobacterium tumefaciens strain EHA105, overexpression of heterogenous LcF3H enhances the expression levels of downstream genes but does not cause alteration in the expression of upstream genes including endogenous F3H of Nicotiana tabacum. The enzyme's overexpression does cause a significant increase in the content of three major flavan-3-ols, catechin, epicatechin and epigallocatechin
Lycium chinense
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
a (2S)-flavan-4-one + 2-oxoglutarate + O2
Lycium chinense
-
a (2R,3R)-dihydroflavonol + succinate + CO2
-
-
?
naringenin + 2-oxoglutarate + O2
Lycium chinense
-
dihydrokaempferol + succinate + CO2
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
flower
-
Lycium chinense
-
leaf
primordial leaf, young leaf (highest level), and mature leaf
Lycium chinense
-
additional information
tissue pattern of enzyme expression, overview
Lycium chinense
-
root
low expression level
Lycium chinense
-
seedling
-
Lycium chinense
-
stem
-
Lycium chinense
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
a (2S)-flavan-4-one + 2-oxoglutarate + O2
-
746138
Lycium chinense
a (2R,3R)-dihydroflavonol + succinate + CO2
-
-
-
?
naringenin + 2-oxoglutarate + O2
-
746138
Lycium chinense
dihydrokaempferol + succinate + CO2
-
-
-
?
Expression
Organism
Commentary
Expression
Lycium chinense
the transcript levels of LcF3H shows significant decrease when plants are treated by exogenous nordihydroguaiaretic acid (100 mM) application together with drought stress treatment comparing to that treated with drought stress alone, the decrease in LcF3H expression under drought stress is less in overexpressing lines compared to the control plants
down
Lycium chinense
positive correlation between absisic acid and LcF3H expression level
additional information
General Information
General Information
Commentary
Organism
metabolism
positive correlation between absisic acid and LcF3H expression level
Lycium chinense
physiological function
the flavanone 3-hydroxylase (F3H) gene which encodes flavanone 3-hydroxylase, is essential in flavonoids biosynthetic pathway. Lycium chinense (L. chinense) is a deciduous woody perennial halophyte that grows under a large variety of environmental conditions and survives under extreme drought stress, possible relationship between the oxidative damage and the regulation of LcF3H gene expression in Lycium chinense under drought stress
Lycium chinense
General Information (protein specific)
General Information
Commentary
Organism
metabolism
positive correlation between absisic acid and LcF3H expression level
Lycium chinense
physiological function
the flavanone 3-hydroxylase (F3H) gene which encodes flavanone 3-hydroxylase, is essential in flavonoids biosynthetic pathway. Lycium chinense (L. chinense) is a deciduous woody perennial halophyte that grows under a large variety of environmental conditions and survives under extreme drought stress, possible relationship between the oxidative damage and the regulation of LcF3H gene expression in Lycium chinense under drought stress
Lycium chinense
Expression (protein specific)
Organism
Commentary
Expression
Lycium chinense
the transcript levels of LcF3H shows significant decrease when plants are treated by exogenous nordihydroguaiaretic acid (100 mM) application together with drought stress treatment comparing to that treated with drought stress alone, the decrease in LcF3H expression under drought stress is less in overexpressing lines compared to the control plants
down
Lycium chinense
positive correlation between absisic acid and LcF3H expression level
additional information
Other publictions for EC 1.14.11.9
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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745484
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Molecular cloning and functio ...
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745623
Khumkarjorn
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Cloning and expression analys ...
x Ascocenda, x Ascocenda NK-2012
J. Plant Biochem. Biotechnol.
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2017
1
-
1
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4
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4
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2
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4
1
1
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1
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1
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1
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746071
Li
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PnF3H, a flavanone 3-hydroxyl ...
Pohlia nutans
Plant Growth Regul.
83
489-500
2017
1
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1
-
1
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1
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2
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1
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1
1
1
1
-
-
744604
Tu
Molecular characterization of ...
Carthamus tinctorius
BMC Plant Biol.
16
132
2016
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1
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3
1
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6
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1
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1
1
2
2
1
-
-
746122
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Artemisia annua
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105
29-36
2016
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1
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1
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746138
Song
Molecular cloning and identif ...
Lycium chinense
Plant Physiol. Biochem.
98
89-100
2016
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1
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2
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2
2
2
2
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745621
Kumar
-
Functional characterization o ...
Phyllanthus emblica
J. Plant Biochem. Biotechnol.
24
453-460
2015
1
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1
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1
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3
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742549
Flores
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Fragaria x ananassa, Ribes nigrum, Ribes rubrum, Rubus idaeus, Rubus plicatus, Vaccinium myrtillus
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153
130-133
2014
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5
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8
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11
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15
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13
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15
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5
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5
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743524
Li
Flower color patterning in pa ...
Viola x wittrockiana
Plant Physiol. Biochem.
84
134-141
2014
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1
1
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743971
Zhang
-
Cloning and functional analys ...
Reaumuria trigyna
Acta Physiol. Plant.
36
1221-1229
2014
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1
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2
2
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1
3
6
2
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746091
Mahajan
Overexpression of a tea flava ...
Camellia sinensis, Camellia sinensis UPASI-10
Plant Mol. Biol.
85
551-573
2014
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1
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1
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6
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1
1
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726211
Liu
Regulation of flavanone 3-hydr ...
Hololachna songarica
Plant Physiol. Biochem.
73C
161-167
2013
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1
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1
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726318
Cheng
Diversifying selection on flav ...
Glycine max, Glycine soja
PLoS ONE
8
e54154
2013
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2
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4
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726200
Flachowsky
Silencing of flavanone-3-hydro ...
Malus domestica
Plant Physiol. Biochem.
51
18-25
2012
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1
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1
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1
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3
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1
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1
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1
2
2
1
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725165
Himi
Differential expression of thr ...
Triticum aestivum
Int. J. Plant Genomics
2011
369460
2011
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3
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10
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3
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3
9
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713238
Lovdal
Synergetic effects of nitrogen ...
Solanum lycopersicum, Solanum lycopersicum Suzanne
Phytochemistry
71
605-613
2010
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1
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2
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1
1
1
1
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713256
Liu
Molecular dissection of the pa ...
Sorghum bicolor
Plant Cell Physiol.
51
1173-1185
2010
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1
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3
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1
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1
3
3
1
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698431
Halbwirth
Measuring flavonoid enzyme act ...
no activity in Actinidia deliciosa, Prunus avium, Prunus cerasus, Prunus domestica, Ribes uva-crispa, Rubus idaeus, Rubus plicatus, Sambucus nigra
J. Agric. Food Chem.
57
4983-4987
2009
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7
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14
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9
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-
7
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22
28
-
21
-
21
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21
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21
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7
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14
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7
-
22
28
-
21
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-
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-
-
700130
Cheng
-
Polymorphisms of soybean isofl ...
Glycine max, Glycine soja
Mol. Breed.
25
13-24
2009
-
2
2
-
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-
-
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2
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2
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2
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4
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2
2
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2
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-
2
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
700225
Khlestkina
Anthocyanin biosynthesis genes ...
Secale cereale
Mol. Genet. Genomics
282
475-485
2009
-
-
1
-
-
-
-
-
-
-
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4
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2
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1
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-
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-
-
-
-
-
-
-
-
-
-
-
-
700742
Zheng
-
Sugars induce anthocyanin accu ...
Vitis vinifera
Plant Growth Regul.
58
251-260
2009
1
1
-
-
-
-
-
-
-
-
1
-
-
1
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1
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3
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1
1
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1
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1
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-
-
-
-
-
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-
700794
Eungwanichayapant
Accumulation of catechins in t ...
Camellia sinensis var. sinensis
Plant Physiol. Biochem.
47
94-97
2009
-
1
-
-
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1
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2
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2
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2
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1
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-
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-
688942
Kim
Accumulation of flavonols in r ...
Glycine max
Mol. Cells
25
247-252
2008
-
-
1
-
-
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-
1
-
1
2
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1
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4
1
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1
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4
1
-
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-
-
-
-
-
-
-
-
-
-
-
688943
Kim
Flavanone 3beta-hydroxylases f ...
Oryza sativa
Mol. Cell
25
312-316
2008
-
-
1
-
-
-
1
3
-
-
3
-
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5
-
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1
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12
1
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1
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3
3
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3
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3
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3
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3
-
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12
3
-
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-
-
-
-
-
-
-
-
-
-
-
-
689653
Owens
Biochemical and genetic charac ...
Arabidopsis thaliana
Plant Physiol. Biochem.
46
833-843
2008
-
-
-
-
1
-
-
1
-
-
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4
-
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1
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1
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1
-
1
-
1
-
1
-
1
-
-
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-
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-
-
-
-
690168
Singh
An early gene of the flavonoid ...
Camellia sinensis
Tree Physiol.
28
1349-1356
2008
-
-
1
-
-
-
-
-
-
-
-
-
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3
-
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1
1
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1
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1
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1
1
-
1
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-
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-
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-
-
-
-
-
-
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-
700308
Hukkanen
Benzothiadiazole affects the l ...
Rubus arcticus
Mol. Plant Pathol.
9
799-808
2008
-
-
-
-
-
-
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4
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1
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1
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-
-
-
-
-
-
701213
Nakatsuka
-
Isolation and expression analy ...
Rhododendron x pulchrum
Sci. Hortic.
118
314-320
2008
-
-
1
-
-
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-
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1
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4
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2
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1
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4
-
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-
-
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1
1
1
1
-
-
746540
Singh
An early gene of the flavonoi ...
Camellia sinensis
Tree Physiol.
28
1349-1356
2008
-
-
1
-
-
-
-
-
-
-
-
1
-
3
-
-
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-
-
1
1
-
1
1
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1
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-
-
1
-
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1
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1
1
-
1
1
-
-
-
-
-
-
-
1
2
-
-
2
-
-
689577
Gebhardt
Evolution of flavone synthase ...
Petroselinum crispum
Plant Physiol.
144
1442-1454
2007
-
-
1
-
16
-
-
-
-
-
-
-
-
4
-
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-
-
-
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1
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1
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16
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1
-
-
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-
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-
-
-
-
-
-
-
671524
Miyahisa
Combinatorial biosynthesis of ...
Citrus sinensis
Appl. Microbiol. Biotechnol.
71
53-58
2006
-
-
1
-
-
-
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-
-
-
-
-
-
6
-
-
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-
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1
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-
-
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-
-
-
-
674117
Halbwirth
Two-phase flavonoid formation ...
Fragaria x ananassa
J. Agric. Food Chem.
54
1479-1485
2006
-
-
-
-
-
-
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3
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1
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1
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1
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2
-
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-
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-
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-
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-
-
-
-
-
-
675792
Leonard
Functional expression of a P45 ...
Malus domestica
Metab. Eng.
8
172-181
2006
-
-
1
-
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-
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4
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1
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2
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-
-
676671
Halbwirth
-
Screening for inhibitors of 2- ...
Malus domestica, Pyrus communis
Plant Sci.
171
194-205
2006
12
-
2
-
-
-
22
-
-
2
-
-
-
2
-
-
-
-
-
2
-
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4
-
6
2
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2
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-
2
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12
-
2
2
-
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-
-
22
-
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2
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2
-
-
4
-
2
-
-
-
2
-
-
-
-
-
-
-
-
-
658733
Wellmann
Significance of C-terminal seq ...
Petunia x hybrida
FEBS Lett.
561
149-154
2004
-
-
1
-
1
-
-
-
-
-
-
1
-
3
-
-
1
-
-
-
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4
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2
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1
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1
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1
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1
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-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
655572
Martens
Divergent evolution of flavono ...
Petroselinum crispum
FEBS Lett.
544
93-98
2003
-
-
1
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
1
-
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-
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1
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1
-
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
660098
Pelt
Flavanone 3-hydroxylase expres ...
Citrus x paradisi, Petunia sp.
Phytochemistry
64
435-444
2003
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
10
-
-
-
-
2
-
-
-
-
-
-
-
-
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-
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-
-
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-
10
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439125
Lukacin
Site-directed mutagenesis of t ...
Petunia x hybrida
Eur. J. Biochem.
267
853-860
2000
-
-
1
-
3
-
-
4
1
-
-
1
-
4
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
3
-
-
-
-
4
1
-
-
1
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439127
Lukacin
The monomeric polypeptide comp ...
Petunia x hybrida
FEBS Lett.
467
353-358
2000
-
-
-
-
-
-
-
-
-
-
1
-
-
5
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439124
Lukacin
Identification of strictly con ...
Petunia x hybrida
Eur. J. Biochem.
249
748-757
1997
-
-
1
-
5
-
1
2
1
2
-
1
-
3
-
-
1
-
-
-
1
-
3
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
5
-
-
1
-
2
1
2
-
1
-
-
-
1
-
-
1
-
3
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
439128
Pelletier
Analysis of flavanone 3-hydrox ...
Arabidopsis thaliana
Plant Physiol.
111
339-345
1996
-
-
1
-
-
-
-
-
-
-
-
1
-
4
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439122
Charrier
Molecular characterization and ...
Medicago sativa
Plant Mol. Biol.
29
773-786
1995
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439126
Britsch
Molecular characterization of ...
Antirrhinum majus, Callistephus chinensis, Dianthus caryophyllus, Hordeum vulgare, Matthiola incana, Petunia x hybrida
Eur. J. Biochem.
217
745-754
1993
-
-
3
-
-
-
2
-
-
-
2
-
-
6
-
-
1
-
-
3
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
2
-
-
-
-
2
-
-
-
-
1
-
3
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439113
Britsch
Purification and characterizat ...
Petroselinum crispum
Arch. Biochem. Biophys.
282
152-160
1990
1
-
-
-
-
-
3
3
-
1
1
-
-
1
-
-
1
1
-
-
1
-
3
1
-
-
-
-
-
1
-
-
-
1
-
-
1
-
-
-
-
-
-
-
3
1
3
-
1
1
-
-
-
-
1
-
-
1
-
3
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
439114
Britsch
Purification of flavanone 3 be ...
Petunia x hybrida
Arch. Biochem. Biophys.
276
348-354
1990
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439123
O'Neill
Molecular genetic analysis of ...
Solanum lycopersicum
Mol. Gen. Genet.
224
279-288
1990
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439115
Beerhues
-
Flavanone 3-hydroxylase and di ...
Tulipa hybrid cultivar
J. Plant Physiol.
133
743-746
1989
-
-
-
-
-
-
4
-
-
1
-
-
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
1
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
439116
Takeda
-
Anthocyanin composition of Sin ...
Sinapis alba
Phytochemistry
27
1351-1353
1988
-
-
-
-
-
-
-
-
-
-
-
1
-
1
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1
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1
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-
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-
-
-
-
-
-
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-
-
-
-
-
-
-
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-
1
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-
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-
1
-
-
1
-
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-
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-
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-
439119
Britsch
Purification and characterizat ...
Petunia x hybrida
Eur. J. Biochem.
156
569-577
1986
2
-
-
-
-
-
10
4
-
2
3
-
-
2
1
-
1
-
-
1
1
1
3
1
-
-
-
-
-
1
-
-
-
2
-
-
2
-
-
-
-
-
-
-
10
2
4
-
2
3
-
-
1
-
1
-
1
1
1
3
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
439120
Forkmann
Selection and characterisation ...
Dahlia pinnata, Glandularia x hybrida, Streptocarpus hybrida, Zinnia elegans
Planta
161
261-265
1984
-
-
-
-
-
-
12
-
4
-
-
4
-
7
-
-
-
-
-
4
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
12
-
-
4
-
-
4
-
-
-
-
-
4
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
438998
Dangelmayr
-
Relationship between flower de ...
Matthiola incana
Z. Naturforsch. C
38
551-555
1983
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
4
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
4
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439001
Forkmann
-
Genetic control of flavanone 3 ...
Antirrhinum majus
Z. Naturforsch. C
36
411-416
1981
-
-
-
-
-
-
-
-
1
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-
-
-
1
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-
-
1
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-
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-
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-
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1
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1
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439006
Forkmann
-
Anthocyanin biosynthesis in fl ...
Matthiola incana
Z. Naturforsch. C
35
691-695
1980
1
-
-
-
-
-
5
-
1
1
-
-
-
1
-
-
-
-
-
1
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
5
-
-
1
1
-
-
-
-
-
-
-
1
1
-
2
-
-
-
-
-
-
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-