Cloned (Comment) | Organism |
---|---|
gene F3H, DNA and amino acid sequence determination and analysis, phylogenetic tree, semi-quantitative RT-PCR enzyme expression analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Artemisia annua |
Protein Variants | Comment | Organism |
---|---|---|
biotecnology | AaF3H is a potential target for regulation of flavonoids biosynthesis in Artemisia annua through metabolic engineering | Artemisia annua |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics | Artemisia annua | |
0.218 | - |
naringenin | pH 8.5, 35°C, recombinant His-tagged enzyme | Artemisia annua |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | required for activity | Artemisia annua |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
naringenin + 2-oxoglutarate + O2 | Artemisia annua | - |
dihydrokaempferol + succinate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Artemisia annua | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Artemisia annua |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | the AaF3H transcripts are accumulated in the cultivar with higher level of flavonoids than that with lower level of flavonoids | Artemisia annua | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
naringenin + 2-oxoglutarate + O2 | - |
Artemisia annua | dihydrokaempferol + succinate + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 41180, sequence calculation | Artemisia annua |
Synonyms | Comment | Organism |
---|---|---|
AaF3H | - |
Artemisia annua |
F3H | - |
Artemisia annua |
flavanone 3-hydroxylase | - |
Artemisia annua |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Artemisia annua |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Artemisia annua |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Artemisia annua | sequence calculation | - |
5.67 |