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Literature summary for 1.14.11.9 extracted from
- Molecular cloning and characterization of a flavanone 3-hydroxylase gene from Artemisia annua L. (2016), Plant Physiol. Biochem., 105, 29-36 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene F3H, DNA and amino acid sequence determination and analysis, phylogenetic tree, semi-quantitative RT-PCR enzyme expression analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Artemisia annua |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| biotecnology | AaF3H is a potential target for regulation of flavonoids biosynthesis in Artemisia annua through metabolic engineering | Artemisia annua |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Michaelis-Menten kinetics | Artemisia annua | |
| 0.218 | - |
naringenin | pH 8.5, 35°C, recombinant His-tagged enzyme | Artemisia annua |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | required for activity | Artemisia annua | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| naringenin + 2-oxoglutarate + O2 | Artemisia annua | - |
dihydrokaempferol + succinate + CO2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Artemisia annua | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Artemisia annua |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | the AaF3H transcripts are accumulated in the cultivar with higher level of flavonoids than that with lower level of flavonoids | Artemisia annua | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| naringenin + 2-oxoglutarate + O2 | - |
Artemisia annua | dihydrokaempferol + succinate + CO2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 41180, sequence calculation | Artemisia annua |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AaF3H | - |
Artemisia annua |
| F3H | - |
Artemisia annua |
| flavanone 3-hydroxylase | - |
Artemisia annua |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
- |
Artemisia annua |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Artemisia annua |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Artemisia annua | sequence calculation | - |
5.67 |
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Release 2023.1 (January 2023)
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