Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ascorbate | required | Pohlia nutans |
Cloned (Comment) | Organism |
---|---|
gene PnF3H, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis and tree, quantitative real-time PCR enzyme expression analysis, functional recombinant expression in Arabidopsis thaliana ecotype Col-0 via Agrobacterium tumefaciens-mediated transformation conferring tolerance to salt stress and abscisic acid treatment in transgenic Arabidopsis, recombinant expression of GFP-tagged enzyme in Arabidopsis thaliana | Pohlia nutans |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of transgenic Arabidospis thaliana plants expressing the enzyme from Pohlia nutans, the Agrobacterium tumefaciens-mediated transformation of gene PnF3H confers tolerance to salt stress and abscisic acid treatment in transgenic Arabidopsis, naringenin-feeding experiments and flavonoid metabolism profiling by HPLC analysis, phenotype, overview | Pohlia nutans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | required for activity | Pohlia nutans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
a (2S)-flavan-4-one + 2-oxoglutarate + O2 | Pohlia nutans | - |
a (2R,3R)-dihydroflavonol + succinate + CO2 | - |
? | |
naringenin + 2-oxoglutarate + O2 | Pohlia nutans | - |
dihydrokaempferol + succinate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pohlia nutans | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
a (2S)-flavan-4-one + 2-oxoglutarate + O2 | - |
Pohlia nutans | a (2R,3R)-dihydroflavonol + succinate + CO2 | - |
? | |
naringenin + 2-oxoglutarate + O2 | - |
Pohlia nutans | dihydrokaempferol + succinate + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
F3H | - |
Pohlia nutans |
flavanone 3-hydroxylase | - |
Pohlia nutans |
PnF3H | - |
Pohlia nutans |
Organism | Comment | Expression |
---|---|---|
Pohlia nutans | PnF3H expression levels increase by 2.49fold at 1 h and then slightly decrease to 1.47fold at 24 h with cold treatment. For the simulated drought stress (i.e. 20% PEG 6000), the PnF3H expression levels enhance by 2.39fold at 3 h and the transcript abundance remains high up to 12 h. In the UV-B radiation, the PnF3H expression levels increase and reach the maximum level of 4.57fold at 3 h. After exogenous abscisic acid treatment, the PnF3H expression levels increase quickly and reach the maximum level of 2.57fold at 0.5 h | up |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme plays a role in plant salt stress resistance. PnF3H transcripts are induced by various stress and may play a positive role in stress tolerance | Pohlia nutans |