BRENDA - Reference to 1.14.11.9; Id = 745623

ascorbate

required

x Ascocenda

gene F3H cloned from petals, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis and tree, quantitative RT-PCR enzyme expression analysis, recombinant expression in Escherichia coli strain BL21

x Ascocenda

0.207

-

naringenin

pH 8.0, 45°C

x Ascocenda

Fe2+

required for catalytic activity

x Ascocenda

a (2S)-flavan-4-one + 2-oxoglutarate + O2

x Ascocenda

-

a (2R,3R)-dihydroflavonol + succinate + CO2

-

?

a (2S)-flavan-4-one + 2-oxoglutarate + O2

x Ascocenda NK-2012

-

a (2R,3R)-dihydroflavonol + succinate + CO2

-

?

naringenin + 2-oxoglutarate + O2

x Ascocenda

-

dihydrokaempferol + succinate + CO2

-

?

naringenin + 2-oxoglutarate + O2

x Ascocenda NK-2012

-

dihydrokaempferol + succinate + CO2

-

?

x Ascocenda

J9QYL0

var. Subun

-

x Ascocenda NK-2012

J9QYL0

var. Subun

-

flower

enzyme expression analysis of AcF3H at different developmental stages and in different parts of the flower, overview. The highest enzyme expression occurs when the petals turned pale-orange

x Ascocenda

-

petal

-

x Ascocenda

-

a (2S)-flavan-4-one + 2-oxoglutarate + O2

-

745623

x Ascocenda

a (2R,3R)-dihydroflavonol + succinate + CO2

-

?

a (2S)-flavan-4-one + 2-oxoglutarate + O2

-

745623

x Ascocenda NK-2012

a (2R,3R)-dihydroflavonol + succinate + CO2

-

?

naringenin + 2-oxoglutarate + O2

-

745623

x Ascocenda

dihydrokaempferol + succinate + CO2

-

?

naringenin + 2-oxoglutarate + O2

-

745623

x Ascocenda NK-2012

dihydrokaempferol + succinate + CO2

-

?

x * 41220, sequence calculation, x * 41500, recombinant enzyme, SDS-PAGE

x Ascocenda

AcF3H

-

x Ascocenda

F3H

-

x Ascocenda

flavanone 3-hydroxylase

-

x Ascocenda

35

-

recombinant enzyme

x Ascocenda

20

50

recombinant enzyme, stable at 20-45°C, loss of activity above 45°C, inactivation at 60°C

x Ascocenda

8

-

recombinant enzyme

x Ascocenda

sequence calculation

-

5.1

ascorbate

required

x Ascocenda

gene F3H cloned from petals, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis and tree, quantitative RT-PCR enzyme expression analysis, recombinant expression in Escherichia coli strain BL21

x Ascocenda

0.207

-

naringenin

pH 8.0, 45°C

x Ascocenda

Fe2+

required for catalytic activity

x Ascocenda

a (2S)-flavan-4-one + 2-oxoglutarate + O2

x Ascocenda

-

a (2R,3R)-dihydroflavonol + succinate + CO2

-

?

a (2S)-flavan-4-one + 2-oxoglutarate + O2

x Ascocenda NK-2012

-

a (2R,3R)-dihydroflavonol + succinate + CO2

-

?

naringenin + 2-oxoglutarate + O2

x Ascocenda

-

dihydrokaempferol + succinate + CO2

-

?

naringenin + 2-oxoglutarate + O2

x Ascocenda NK-2012

-

dihydrokaempferol + succinate + CO2

-

?

flower

enzyme expression analysis of AcF3H at different developmental stages and in different parts of the flower, overview. The highest enzyme expression occurs when the petals turned pale-orange

x Ascocenda

-

petal

-

x Ascocenda

-

a (2S)-flavan-4-one + 2-oxoglutarate + O2

-

745623

x Ascocenda

a (2R,3R)-dihydroflavonol + succinate + CO2

-

?

a (2S)-flavan-4-one + 2-oxoglutarate + O2

-

745623

x Ascocenda NK-2012

a (2R,3R)-dihydroflavonol + succinate + CO2

-

?

naringenin + 2-oxoglutarate + O2

-

745623

x Ascocenda

dihydrokaempferol + succinate + CO2

-

?

naringenin + 2-oxoglutarate + O2

-

745623

x Ascocenda NK-2012

dihydrokaempferol + succinate + CO2

-

?

x * 41220, sequence calculation, x * 41500, recombinant enzyme, SDS-PAGE

x Ascocenda

35

-

recombinant enzyme

x Ascocenda

20

50

recombinant enzyme, stable at 20-45°C, loss of activity above 45°C, inactivation at 60°C

x Ascocenda

8

-

recombinant enzyme

x Ascocenda

sequence calculation

-

5.1

742957

Kallscheuer

Functional expression of plan ...

Petunia x hybrida

J. Biotechnol.

258

190-196

2017

-

1

-

1

-

1

-

5

-

3

-

2

-

2

-

1

-

1

-

3

-

2

-

744729

Chaipanya

-

Isolation and expression anal ...

Nelumbo nucifera, Nymphaea hybrid cultivar

Chiang Mai J. Sci.

44

427-437

2017

-

2

-

2

-

2

-

2

-

6

-

2

-

4

-

2

-

2

-

2

-

6

-

2

-

2

-

745484

Zhang

-

Molecular cloning and functio ...

Vaccinium myrtillus

J. Hortic. Sci. Biotechnol.

92

57-64

2017

-

1

-

1

-

4

-

3

-

1

-

4

-

1

-

745623

Khumkarjorn

-

Cloning and expression analys ...

x Ascocenda, x Ascocenda NK-2012

J. Plant Biochem. Biotechnol.

26

179-190

2017

1

-

1

-

1

-

1

-

4

-

2

-

2

-

4

1

3

1

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

4

-

2

-

4

1

-

1

-

1

-

1

-

746071

Li

-

PnF3H, a flavanone 3-hydroxyl ...

Pohlia nutans

Plant Growth Regul.

83

489-500

2017

1

-

1

-

1

-

1

-

2

-

1

-

2

-

3

-

1

-

1

-

1

-

1

-

2

-

2

-

1

-

744604

Tu

Molecular characterization of ...

Carthamus tinctorius

BMC Plant Biol.

16

132

2016

-

1

-

3

1

-

1

-

6

-

1

-

1

-

4

1

3

-

1

-

1

-

3

1

-

1

-

1

-

1

-

4

1

-

1

2

1

-

746122

Xiong

Molecular cloning and charact ...

Artemisia annua

Plant Physiol. Biochem.

105

29-36

2016

-

1

-

1

-

2

-

1

-

1

-

2

-

1

-

1

-

1

3

1

-

1

-

1

-

1

-

1

-

2

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

746138

Song

Molecular cloning and identif ...

Lycium chinense

Plant Physiol. Biochem.

98

89-100

2016

-

1

-

2

-

4

-

6

-

2

-

2

-

1

-

2

-

6

-

2

-

2

-

745621

Kumar

-

Functional characterization o ...

Phyllanthus emblica

J. Plant Biochem. Biotechnol.

24

453-460

2015

1

-

1

-

1

-

3

-

1

-

3

-

3

1

3

1

-

1

-

1

-

1

-

1

-

3

-

3

-

3

1

-

1

-

3

-

742549

Flores

Phenylalanine ammonia-lyase, ...

Fragaria x ananassa, Ribes nigrum, Ribes rubrum, Rubus idaeus, Rubus plicatus, Vaccinium myrtillus

Food Chem.

153

130-133

2014

-

5

-

5

-

8

-

11

-

15

-

15

5

-

5

-

5

-

5

-

13

-

15

-

5

-

5

-

743524

Li

Flower color patterning in pa ...

Viola x wittrockiana

Plant Physiol. Biochem.

84

134-141

2014

-

2

-

2

-

2

-

1

-

743971

Zhang

-

Cloning and functional analys ...

Reaumuria trigyna

Acta Physiol. Plant.

36

1221-1229

2014

-

1

-

2

-

2

-

3

-

2

1

6

-

2

-

2

-

6

-

2

-

1

3

6

2

-

746091

Mahajan

Overexpression of a tea flava ...

Camellia sinensis, Camellia sinensis UPASI-10

Plant Mol. Biol.

85

551-573

2014

-

1

-

6

-

5

-

1

-

6

-

2

-

1

-

6

-

1

-

6

-

1

-

726211

Liu

Regulation of flavanone 3-hydr ...

Hololachna songarica

Plant Physiol. Biochem.

73C

161-167

2013

-

1

-

1

-

4

-

1

2

-

1

-

1

-

1

-

1

-

1

-

726318

Cheng

Diversifying selection on flav ...

Glycine max, Glycine soja

PLoS ONE

8

e54154

2013

-

2

-

3

-

4

-

2

-

4

-

726200

Flachowsky

Silencing of flavanone-3-hydro ...

Malus domestica

Plant Physiol. Biochem.

51

18-25

2012

-

1

-

1

-

1

-

1

-

2

-

3

-

3

-

3

1

-

1

-

1

-

1

-

1

-

1

-

2

-

3

-

1

-

1

-

1

2

1

-

725165

Himi

Differential expression of thr ...

Triticum aestivum

Int. J. Plant Genomics

2011

369460

2011

-

1

-

1

-

3

-

5

-

4

-

3

-

6

-

3

-

3

-

3

-

10

-

3

-

3

9

-

713238

Lovdal

Synergetic effects of nitrogen ...

Solanum lycopersicum, Solanum lycopersicum Suzanne

Phytochemistry

71

605-613

2010

-

1

-

2

-

2

-

1

-

2

-

2

-

1

-

2

-

1

-

2

-

1

-

713256

Liu

Molecular dissection of the pa ...

Sorghum bicolor

Plant Cell Physiol.

51

1173-1185

2010

-

1

-

3

-

1

-

2

-

1

-

1

-

1

3

1

-

698431

Halbwirth

Measuring flavonoid enzyme act ...

no activity in Actinidia deliciosa, Prunus avium, Prunus cerasus, Prunus domestica, Ribes uva-crispa, Rubus idaeus, Rubus plicatus, Sambucus nigra

J. Agric. Food Chem.

57

4983-4987

2009

-

7

-

14

-

9

-

7

-

22

28

-

21

-

21

-

21

-

21

-

7

-

14

-

7

-

22

28

-

21

-

700130

Cheng

-

Polymorphisms of soybean isofl ...

Glycine max, Glycine soja

Mol. Breed.

25

13-24

2009

-

2

-

2

-

2

-

2

-

4

-

2

-

2

-

2

-

700225

Khlestkina

Anthocyanin biosynthesis genes ...

Secale cereale

Mol. Genet. Genomics

282

475-485

2009

-

1

-

4

-

2

-

1

-

700742

Zheng

-

Sugars induce anthocyanin accu ...

Vitis vinifera

Plant Growth Regul.

58

251-260

2009

1

-

1

-

1

-

1

-

1

3

-

1

-

1

-

1

-

1

-

700794

Eungwanichayapant

Accumulation of catechins in t ...

Camellia sinensis var. sinensis

Plant Physiol. Biochem.

47

94-97

2009

-

1

-

1

-

2

-

2

-

1

-

1

-

1

-

2

-

1

-

688942

Kim

Accumulation of flavonols in r ...

Glycine max

Mol. Cells

25

247-252

2008

-

1

-

1

-

1

2

-

1

-

4

1

-

1

-

1

-

1

2

-

4

1

-

688943

Kim

Flavanone 3beta-hydroxylases f ...

Oryza sativa

Mol. Cell

25

312-316

2008

-

1

-

1

3

-

3

-

5

-

1

-

12

1

-

1

-

3

-

3

-

3

-

3

-

3

-

12

3

-

689653

Owens

Biochemical and genetic charac ...

Arabidopsis thaliana

Plant Physiol. Biochem.

46

833-843

2008

-

1

-

1

-

4

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

690168

Singh

An early gene of the flavonoid ...

Camellia sinensis

Tree Physiol.

28

1349-1356

2008

-

1

-

3

-

1

-

1

-

1

-

1

-

1

-

700308

Hukkanen

Benzothiadiazole affects the l ...

Rubus arcticus

Mol. Plant Pathol.

9

799-808

2008

-

4

-

1

-

1

-

1

-

701213

Nakatsuka

-

Isolation and expression analy ...

Rhododendron x pulchrum

Sci. Hortic.

118

314-320

2008

-

1

-

1

-

4

-

2

-

1

-

4

-

1

-

746540

Singh

An early gene of the flavonoi ...

Camellia sinensis

Tree Physiol.

28

1349-1356

2008

-

1

-

1

-

3

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

1

2

-

2

-

689577

Gebhardt

Evolution of flavone synthase ...

Petroselinum crispum

Plant Physiol.

144

1442-1454

2007

-

1

-

16

-

4

-

1

-

1

-

16

-

1

-

671524

Miyahisa

Combinatorial biosynthesis of ...

Citrus sinensis

Appl. Microbiol. Biotechnol.

71

53-58

2006

-

1

-

6

-

1

-

2

-

1

-

1

-

674117

Halbwirth

Two-phase flavonoid formation ...

Fragaria x ananassa

J. Agric. Food Chem.

54

1479-1485

2006

-

3

-

1

-

1

-

2

-

2

-

1

-

1

-

2

-

675792

Leonard

Functional expression of a P45 ...

Malus domestica

Metab. Eng.

8

172-181

2006

-

1

-

4

-

2

-

2

-

1

-

2

-

676671

Halbwirth

-

Screening for inhibitors of 2- ...

Malus domestica, Pyrus communis

Plant Sci.

171

194-205

2006

12

-

2

-

22

-

2

-

2

-

2

-

4

-

6

2

-

2

-

2

-

12

-

2

-

22

-

2

-

2

-

4

-

2

-

2

-

658733

Wellmann

Significance of C-terminal seq ...

Petunia x hybrida

FEBS Lett.

561

149-154

2004

-

1

-

1

-

1

-

3

-

1

-

4

-

2

-

1

-

1

-

1

-

1

-

4

-

655572

Martens

Divergent evolution of flavono ...

Petroselinum crispum

FEBS Lett.

544

93-98

2003

-

1

-

4

-

1

-

2

-

1

-

1

-

660098

Pelt

Flavanone 3-hydroxylase expres ...

Citrus x paradisi, Petunia sp.

Phytochemistry

64

435-444

2003

-

6

-

10

-

2

-

10

-

439125

Lukacin

Site-directed mutagenesis of t ...

Petunia x hybrida

Eur. J. Biochem.

267

853-860

2000

-

1

-

3

-

4

1

-

1

-

4

-

1

-

1

-

1

-

3

-

4

1

-

1

-

1

-

1

-

439127

Lukacin

The monomeric polypeptide comp ...

Petunia x hybrida

FEBS Lett.

467

353-358

2000

-

1

-

5

-

1

-

1

-

1

-

439124

Lukacin

Identification of strictly con ...

Petunia x hybrida

Eur. J. Biochem.

249

748-757

1997

-

1

-

5

-

1

2

1

2

-

1

-

3

-

1

-

1

-

3

-

2

-

1

-

5

-

1

-

2

1

2

-

1

-

1

-

1

-

3

-

2

-

439128

Pelletier

Analysis of flavanone 3-hydrox ...

Arabidopsis thaliana

Plant Physiol.

111

339-345

1996

-

1

-

1

-

4

-

2

-

1

-

1

-

1

-

2

-

1

-

439122

Charrier

Molecular characterization and ...

Medicago sativa

Plant Mol. Biol.

29

773-786

1995

-

3

-

1

-

1

-

439126

Britsch

Molecular characterization of ...

Antirrhinum majus, Callistephus chinensis, Dianthus caryophyllus, Hordeum vulgare, Matthiola incana, Petunia x hybrida

Eur. J. Biochem.

217

745-754

1993

-

3

-

2

-

2

-

6

-

1

-

3

-

3

-

3

-

2

-

2

-

1

-

3

-

3

-

439113

Britsch

Purification and characterizat ...

Petroselinum crispum

Arch. Biochem. Biophys.

282

152-160

1990

1

-

3

-

1

-

1

-

1

-

1

-

3

1

-

1

-

1

-

1

-

3

1

3

-

1

-

1

-

1

-

3

1

-

1

-

439114

Britsch

Purification of flavanone 3 be ...

Petunia x hybrida

Arch. Biochem. Biophys.

276

348-354

1990

-

1

-

1

-

1

-

1

-

1

-

439123

O'Neill

Molecular genetic analysis of ...

Solanum lycopersicum

Mol. Gen. Genet.

224

279-288

1990

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

439115

Beerhues

-

Flavanone 3-hydroxylase and di ...

Tulipa hybrid cultivar

J. Plant Physiol.

133

743-746

1989

-

4

-

1

-

1

-

1

-

2

-

1

-

4

-

1

-

1

-

2

-

1

-

439116

Takeda

-

Anthocyanin composition of Sin ...

Sinapis alba

Phytochemistry

27

1351-1353

1988

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

439119

Britsch

Purification and characterizat ...

Petunia x hybrida

Eur. J. Biochem.

156

569-577

1986

2

-

10

4

-

2

3

-

2

1

-

1

-

1

3

1

-

1

-

2

-

2

-

10

2

4

-

2

3

-

1

-

1

-

1

3

1

-

1

-

439120

Forkmann

Selection and characterisation ...

Dahlia pinnata, Glandularia x hybrida, Streptocarpus hybrida, Zinnia elegans

Planta

161

261-265

1984

-

12

-

4

-

4

-

7

-

4

-

4

-

12

-

4

-

4

-

4

-

4

-

438998

Dangelmayr

-

Relationship between flower de ...

Matthiola incana

Z. Naturforsch. C

38

551-555

1983

-

2

-

1

-

4

-

2

-

2

-

4

-

2

-

439001

Forkmann

-

Genetic control of flavanone 3 ...

Antirrhinum majus

Z. Naturforsch. C

36

411-416

1981

-

1

-

1

-

1

-

1

-

1

-

439006

Forkmann

-

Anthocyanin biosynthesis in fl ...

Matthiola incana

Z. Naturforsch. C

35

691-695

1980

1

-

5

-

1

-

1

-

1

-

2

-

1

-

5

-

1

-

1

-

2

-

Cloning and expression analysis of a flavanone 3-hydroxylase gene in Ascocenda orchid

Data extracted from this reference: