BRENDA - Enzyme Database
show all sequences of 1.14.11.9

Functional characterization of flavanone 3-hydroxylase gene from Phyllanthus emblica (L.)

Kumar, A.; Singh, B.; Singh, K.; J. Plant Biochem. Biotechnol. 24, 453-460 (2015)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
ascorbate
required for activity
Phyllanthus emblica
Cloned(Commentary)
Cloned (Commentary)
Organism
gene PeF3H, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, semi-quantitative RT-PCR enzyme expression analysis, recombinant expression in Escherichia coli tsrain M15 pREP-4
Phyllanthus emblica
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Fe2+
required for activity
Phyllanthus emblica
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
a (2S)-flavan-4-one + 2-oxoglutarate + O2
Phyllanthus emblica
-
a (2R,3R)-dihydroflavonol + succinate + CO2
-
-
?
eriodictyol + 2-oxoglutarate + O2
Phyllanthus emblica
-
dihydroquercetin + succinate + CO2
-
-
?
naringenin + 2-oxoglutarate + O2
Phyllanthus emblica
-
dihydrokaempferol + succinate + CO2
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Phyllanthus emblica
T1UPN7
ten year old healthy tree growing in the botanical garden of Panjab University, Chandigarh, India under natural conditions
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
flower
-
Phyllanthus emblica
-
fruit
-
Phyllanthus emblica
-
leaf
-
Phyllanthus emblica
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
a (2S)-flavan-4-one + 2-oxoglutarate + O2
-
745621
Phyllanthus emblica
a (2R,3R)-dihydroflavonol + succinate + CO2
-
-
-
?
eriodictyol + 2-oxoglutarate + O2
-
745621
Phyllanthus emblica
dihydroquercetin + succinate + CO2
-
-
-
?
naringenin + 2-oxoglutarate + O2
-
745621
Phyllanthus emblica
dihydrokaempferol + succinate + CO2
-
-
-
?
Subunits
Subunits
Commentary
Organism
More
three-dimensional protein structure modeling, overview. F3H has a jelly roll in the enzyme core, a typical structure shared by all 2-oxoglutarate-dependent dioxygenases including F3Hs. Position between 194 and 295 amino acids represent the 2-oxoglutarate-ferrous(II) oxygenase superfamily domain which plays key role in catalyzing a redox reaction
Phyllanthus emblica
Synonyms
Synonyms
Commentary
Organism
F3H
-
Phyllanthus emblica
flavanone 3-hydroxylase
-
Phyllanthus emblica
PeF3H
-
Phyllanthus emblica
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
assay at
Phyllanthus emblica
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
assay at
Phyllanthus emblica
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
ascorbate
required for activity
Phyllanthus emblica
Cloned(Commentary) (protein specific)
Commentary
Organism
gene PeF3H, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, semi-quantitative RT-PCR enzyme expression analysis, recombinant expression in Escherichia coli tsrain M15 pREP-4
Phyllanthus emblica
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Fe2+
required for activity
Phyllanthus emblica
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
a (2S)-flavan-4-one + 2-oxoglutarate + O2
Phyllanthus emblica
-
a (2R,3R)-dihydroflavonol + succinate + CO2
-
-
?
eriodictyol + 2-oxoglutarate + O2
Phyllanthus emblica
-
dihydroquercetin + succinate + CO2
-
-
?
naringenin + 2-oxoglutarate + O2
Phyllanthus emblica
-
dihydrokaempferol + succinate + CO2
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
flower
-
Phyllanthus emblica
-
fruit
-
Phyllanthus emblica
-
leaf
-
Phyllanthus emblica
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
a (2S)-flavan-4-one + 2-oxoglutarate + O2
-
745621
Phyllanthus emblica
a (2R,3R)-dihydroflavonol + succinate + CO2
-
-
-
?
eriodictyol + 2-oxoglutarate + O2
-
745621
Phyllanthus emblica
dihydroquercetin + succinate + CO2
-
-
-
?
naringenin + 2-oxoglutarate + O2
-
745621
Phyllanthus emblica
dihydrokaempferol + succinate + CO2
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
More
three-dimensional protein structure modeling, overview. F3H has a jelly roll in the enzyme core, a typical structure shared by all 2-oxoglutarate-dependent dioxygenases including F3Hs. Position between 194 and 295 amino acids represent the 2-oxoglutarate-ferrous(II) oxygenase superfamily domain which plays key role in catalyzing a redox reaction
Phyllanthus emblica
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
assay at
Phyllanthus emblica
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
assay at
Phyllanthus emblica
General Information
General Information
Commentary
Organism
evolution
the enzyme belongs to the Fe(II)- and 2-oxoglutarate-dependent dioxygenase (2-ODD) superfamily, sharing the conserved motif of pfam 03171. F3H has a jelly roll in the enzyme core, a typical structure shared by all 2-oxoglutarate-dependent dioxygenases including F3Hs. Phylogenetic and molecular evolutionary analyses
Phyllanthus emblica
additional information
three-dimensional protein structure modeling, overview
Phyllanthus emblica
physiological function
flavanone 3-hydroxylase (F3H) is an important regulatory enzyme of the flavonoid pathway which catalyzes the stereospecific hydroxylation of (2S)-naringenin to form (2R,3R)-dihydroflavonol. These dihydroflavonols serve as intermediates for the biosynthesis of anthocyanidins and flavonols
Phyllanthus emblica
General Information (protein specific)
General Information
Commentary
Organism
evolution
the enzyme belongs to the Fe(II)- and 2-oxoglutarate-dependent dioxygenase (2-ODD) superfamily, sharing the conserved motif of pfam 03171. F3H has a jelly roll in the enzyme core, a typical structure shared by all 2-oxoglutarate-dependent dioxygenases including F3Hs. Phylogenetic and molecular evolutionary analyses
Phyllanthus emblica
additional information
three-dimensional protein structure modeling, overview
Phyllanthus emblica
physiological function
flavanone 3-hydroxylase (F3H) is an important regulatory enzyme of the flavonoid pathway which catalyzes the stereospecific hydroxylation of (2S)-naringenin to form (2R,3R)-dihydroflavonol. These dihydroflavonols serve as intermediates for the biosynthesis of anthocyanidins and flavonols
Phyllanthus emblica
Other publictions for EC
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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1
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1
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4
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1
1
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745623
Khumkarjorn
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Cloning and expression analys ...
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1
-
1
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1
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4
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2
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1
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4
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2
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4
1
1
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1
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1
-
-
1
-
-
-
-
-
-
746071
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Pohlia nutans
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1
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2
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2
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1
1
1
1
-
-
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1
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1
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1
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1
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2
1
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1
1
1
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1
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1
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746138
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1
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2
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4
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6
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2
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2
2
2
2
-
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745621
Kumar
-
Functional characterization o ...
Phyllanthus emblica
J. Plant Biochem. Biotechnol.
24
453-460
2015
1
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3
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3
1
1
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1
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3
3
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5
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5
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8
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11
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15
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15
5
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5
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13
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15
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5
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1
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743971
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Reaumuria trigyna
Acta Physiol. Plant.
36
1221-1229
2014
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1
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2
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2
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1
3
6
2
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746091
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85
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1
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726211
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73C
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726318
Cheng
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PLoS ONE
8
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2013
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2
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4
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726200
Flachowsky
Silencing of flavanone-3-hydro ...
Malus domestica
Plant Physiol. Biochem.
51
18-25
2012
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1
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1
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1
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1
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3
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1
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2
2
1
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725165
Himi
Differential expression of thr ...
Triticum aestivum
Int. J. Plant Genomics
2011
369460
2011
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3
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3
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10
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3
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3
9
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713238
Lovdal
Synergetic effects of nitrogen ...
Solanum lycopersicum, Solanum lycopersicum Suzanne
Phytochemistry
71
605-613
2010
-
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1
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2
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2
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1
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1
1
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1
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713256
Liu
Molecular dissection of the pa ...
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2010
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1
3
3
1
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-
698431
Halbwirth
Measuring flavonoid enzyme act ...
no activity in Actinidia deliciosa, Prunus avium, Prunus cerasus, Prunus domestica, Ribes uva-crispa, Rubus idaeus, Rubus plicatus, Sambucus nigra
J. Agric. Food Chem.
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7
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28
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7
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28
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21
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700130
Cheng
-
Polymorphisms of soybean isofl ...
Glycine max, Glycine soja
Mol. Breed.
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2009
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2
2
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2
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700225
Khlestkina
Anthocyanin biosynthesis genes ...
Secale cereale
Mol. Genet. Genomics
282
475-485
2009
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1
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4
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700742
Zheng
-
Sugars induce anthocyanin accu ...
Vitis vinifera
Plant Growth Regul.
58
251-260
2009
1
1
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1
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700794
Eungwanichayapant
Accumulation of catechins in t ...
Camellia sinensis var. sinensis
Plant Physiol. Biochem.
47
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2009
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1
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688942
Kim
Accumulation of flavonols in r ...
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Mol. Cells
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2008
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4
1
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688943
Kim
Flavanone 3beta-hydroxylases f ...
Oryza sativa
Mol. Cell
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2008
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1
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1
3
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3
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5
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1
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12
1
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1
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3
3
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3
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3
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12
3
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689653
Owens
Biochemical and genetic charac ...
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2008
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1
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1
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1
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1
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1
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690168
Singh
An early gene of the flavonoid ...
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28
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2008
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1
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1
1
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1
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Hukkanen
Benzothiadiazole affects the l ...
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701213
Nakatsuka
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Isolation and expression analy ...
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2008
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746540
Singh
An early gene of the flavonoi ...
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1
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1
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1
1
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1
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689577
Gebhardt
Evolution of flavone synthase ...
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16
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Combinatorial biosynthesis of ...
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1
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674117
Halbwirth
Two-phase flavonoid formation ...
Fragaria x ananassa
J. Agric. Food Chem.
54
1479-1485
2006
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Leonard
Functional expression of a P45 ...
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2
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676671
Halbwirth
-
Screening for inhibitors of 2- ...
Malus domestica, Pyrus communis
Plant Sci.
171
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2006
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22
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2
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12
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2
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22
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2
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2
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4
-
2
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2
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658733
Wellmann
Significance of C-terminal seq ...
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FEBS Lett.
561
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2004
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1
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Martens
Divergent evolution of flavono ...
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660098
Pelt
Flavanone 3-hydroxylase expres ...
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64
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2003
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439125
Lukacin
Site-directed mutagenesis of t ...
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2000
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439127
Lukacin
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Molecular characterization of ...
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Britsch
Purification and characterizat ...
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Britsch
Purification of flavanone 3 be ...
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439123
O'Neill
Molecular genetic analysis of ...
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1
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1
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1
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-
1
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-
1
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-
-
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-
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-
439115
Beerhues
-
Flavanone 3-hydroxylase and di ...
Tulipa hybrid cultivar
J. Plant Physiol.
133
743-746
1989
-
-
-
-
-
-
4
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-
1
-
-
-
1
-
-
-
-
-
1
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-
2
-
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-
-
-
-
1
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-
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-
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-
-
-
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-
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4
-
-
-
1
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-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
1
-
-
-
-
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-
-
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-
439116
Takeda
-
Anthocyanin composition of Sin ...
Sinapis alba
Phytochemistry
27
1351-1353
1988
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-
-
-
-
-
-
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1
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1
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1
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1
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1
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1
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1
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439119
Britsch
Purification and characterizat ...
Petunia x hybrida
Eur. J. Biochem.
156
569-577
1986
2
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-
-
-
-
10
4
-
2
3
-
-
2
1
-
1
-
-
1
1
1
3
1
-
-
-
-
-
1
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-
-
2
-
-
2
-
-
-
-
-
-
-
10
2
4
-
2
3
-
-
1
-
1
-
1
1
1
3
1
-
-
-
-
1
-
-
-
-
-
-
-
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-
439120
Forkmann
Selection and characterisation ...
Dahlia pinnata, Glandularia x hybrida, Streptocarpus hybrida, Zinnia elegans
Planta
161
261-265
1984
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-
-
-
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12
-
4
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-
4
-
7
-
-
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-
-
4
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-
4
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-
-
-
-
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-
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-
-
-
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12
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4
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4
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4
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-
4
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-
-
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-
-
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-
438998
Dangelmayr
-
Relationship between flower de ...
Matthiola incana
Z. Naturforsch. C
38
551-555
1983
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-
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2
-
1
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4
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2
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2
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4
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-
2
-
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-
-
-
-
439001
Forkmann
-
Genetic control of flavanone 3 ...
Antirrhinum majus
Z. Naturforsch. C
36
411-416
1981
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-
-
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-
-
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1
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1
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1
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1
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1
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-
-
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-
439006
Forkmann
-
Anthocyanin biosynthesis in fl ...
Matthiola incana
Z. Naturforsch. C
35
691-695
1980
1
-
-
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-
5
-
1
1
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1
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-
1
1
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2
-
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1
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-
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5
-
-
1
1
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1
1
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2
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