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Literature summary for 1.14.11.9 extracted from

  • Kumar, A.; Singh, B.; Singh, K.
    Functional characterization of flavanone 3-hydroxylase gene from Phyllanthus emblica (L.) (2015), J. Plant Biochem. Biotechnol., 24, 453-460 .
No PubMed abstract available
Search for more literature for 1.14.11.9

Activating Compound

Activating Compound Comment Organism Structure
ascorbate required for activity Phyllanthus emblica

Cloned(Commentary)

Cloned (Comment) Organism
gene PeF3H, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, semi-quantitative RT-PCR enzyme expression analysis, recombinant expression in Escherichia coli tsrain M15 pREP-4 Phyllanthus emblica

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ required for activity Phyllanthus emblica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
a (2S)-flavan-4-one + 2-oxoglutarate + O2 Phyllanthus emblica
-
 a (2R,3R)-dihydroflavonol + succinate + CO2
-
 ?
eriodictyol + 2-oxoglutarate + O2 Phyllanthus emblica
-
 dihydroquercetin + succinate + CO2
-
 ?
naringenin + 2-oxoglutarate + O2 Phyllanthus emblica
-
 dihydrokaempferol + succinate + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Phyllanthus emblica T1UPN7 ten year old healthy tree growing in the botanical garden of Panjab University, Chandigarh, India under natural conditions
-

Source Tissue

Source Tissue Comment Organism Textmining
flower
-
 Phyllanthus emblica
-
fruit
-
 Phyllanthus emblica
-
leaf
-
 Phyllanthus emblica
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
a (2S)-flavan-4-one + 2-oxoglutarate + O2
-
 Phyllanthus emblica a (2R,3R)-dihydroflavonol + succinate + CO2
-
 ?
eriodictyol + 2-oxoglutarate + O2
-
 Phyllanthus emblica dihydroquercetin + succinate + CO2
-
 ?
naringenin + 2-oxoglutarate + O2
-
 Phyllanthus emblica dihydrokaempferol + succinate + CO2
-
 ?

Subunits

Subunits Comment Organism
More three-dimensional protein structure modeling, overview. F3H has a jelly roll in the enzyme core, a typical structure shared by all 2-oxoglutarate-dependent dioxygenases including F3Hs. Position between 194 and 295 amino acids represent the 2-oxoglutarate-ferrous(II) oxygenase superfamily domain which plays key role in catalyzing a redox reaction Phyllanthus emblica

Synonyms

Synonyms Comment Organism
F3H
-
 Phyllanthus emblica
flavanone 3-hydroxylase
-
 Phyllanthus emblica
PeF3H
-
 Phyllanthus emblica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Phyllanthus emblica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Phyllanthus emblica

General Information

General Information Comment Organism
evolution the enzyme belongs to the Fe(II)- and 2-oxoglutarate-dependent dioxygenase (2-ODD) superfamily, sharing the conserved motif of pfam 03171. F3H has a jelly roll in the enzyme core, a typical structure shared by all 2-oxoglutarate-dependent dioxygenases including F3Hs. Phylogenetic and molecular evolutionary analyses Phyllanthus emblica
additional information three-dimensional protein structure modeling, overview Phyllanthus emblica
physiological function flavanone 3-hydroxylase (F3H) is an important regulatory enzyme of the flavonoid pathway which catalyzes the stereospecific hydroxylation of (2S)-naringenin to form (2R,3R)-dihydroflavonol. These dihydroflavonols serve as intermediates for the biosynthesis of anthocyanidins and flavonols Phyllanthus emblica