BRENDA - Enzyme Database
show all sequences of 1.14.11.9

Isolation and expression analysis of the flavanone 3-hydroxylase genes in lotus (Nelumbo nucifera Gaertn.), waterlily (Nymphaea sp.) and transient silencing in waterlily

Chaipanya, C.; Saetiew, K.; Arunyanart, S.; Parinthawong, N.; Chiang Mai J. Sci. 44, 427-437 (2017)
No PubMed abstract available

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
gene F3H, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, semi-quantitative RT-PCR enzyme expression analysis
Nelumbo nucifera
gene F3H, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, semi-quantitative RT-PCR enzyme expression analysis
Nymphaea hybrid cultivar
Engineering
Protein Variants
Commentary
Organism
additional information
method evaluation for RNAi-mediated silencing downregulation of F3H expression, vector pJA8F3H is efficient and can be used as a transformation vector to transiently suppress F3H expression in waterlily or lotus
Nelumbo nucifera
additional information
method evaluation for RNAi-mediated silencing downregulation of F3H expression, introduction of RNAi gene-silencing vector, pJA8F3H, encoding a hairpin F3H RNA, to waterlily petals using the Agrobacterium tumefaciens strain GV3101 infiltration method, F3H expression is analysed at 1 and 3 days post infiltration semi-quantitative RT-PCR, showing that F3H expression is downregulated at 3 dpi in flowers tested of the red petal variety and purplish blue petal variety compared to controls. Vector pJA8F3H is efficient and can be used as a transformation vector to transiently suppress F3H expression in waterlily or lotus
Nymphaea hybrid cultivar
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
a (2S)-flavan-4-one + 2-oxoglutarate + O2
Nelumbo nucifera
-
a (2R,3R)-dihydroflavonol + succinate + CO2
-
-
?
a (2S)-flavan-4-one + 2-oxoglutarate + O2
Nymphaea hybrid cultivar
-
a (2R,3R)-dihydroflavonol + succinate + CO2
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Nelumbo nucifera
V9P4G8
cv. Buntharik (white petal lotus) and cv. Satabankacha (pink petal lotus)
-
Nymphaea hybrid cultivar
V9P4H2
var. St. Louis Gold
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
leaf
low expression level
Nymphaea hybrid cultivar
-
additional information
expression of F3H is specifically regulated in petals and stamens. The F3H expression is slightly diminished in petals of cultivar Satabankacha at the fully-opening stage, whereas it is detected in the cultivar Buntharik only when white petals are tinted with pink.
Nelumbo nucifera
-
petal
-
Nelumbo nucifera
-
petal
-
Nymphaea hybrid cultivar
-
stamen
-
Nelumbo nucifera
-
stamen
-
Nymphaea hybrid cultivar
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
a (2S)-flavan-4-one + 2-oxoglutarate + O2
-
744729
Nelumbo nucifera
a (2R,3R)-dihydroflavonol + succinate + CO2
-
-
-
?
a (2S)-flavan-4-one + 2-oxoglutarate + O2
-
744729
Nymphaea hybrid cultivar
a (2R,3R)-dihydroflavonol + succinate + CO2
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
F3H
-
Nelumbo nucifera
F3H
-
Nymphaea hybrid cultivar
flavanone 3-hydroxylase
-
Nelumbo nucifera
flavanone 3-hydroxylase
-
Nymphaea hybrid cultivar
Cloned(Commentary) (protein specific)
Commentary
Organism
gene F3H, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, semi-quantitative RT-PCR enzyme expression analysis
Nelumbo nucifera
gene F3H, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, semi-quantitative RT-PCR enzyme expression analysis
Nymphaea hybrid cultivar
Engineering (protein specific)
Protein Variants
Commentary
Organism
additional information
method evaluation for RNAi-mediated silencing downregulation of F3H expression, vector pJA8F3H is efficient and can be used as a transformation vector to transiently suppress F3H expression in waterlily or lotus
Nelumbo nucifera
additional information
method evaluation for RNAi-mediated silencing downregulation of F3H expression, introduction of RNAi gene-silencing vector, pJA8F3H, encoding a hairpin F3H RNA, to waterlily petals using the Agrobacterium tumefaciens strain GV3101 infiltration method, F3H expression is analysed at 1 and 3 days post infiltration semi-quantitative RT-PCR, showing that F3H expression is downregulated at 3 dpi in flowers tested of the red petal variety and purplish blue petal variety compared to controls. Vector pJA8F3H is efficient and can be used as a transformation vector to transiently suppress F3H expression in waterlily or lotus
Nymphaea hybrid cultivar
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
a (2S)-flavan-4-one + 2-oxoglutarate + O2
Nelumbo nucifera
-
a (2R,3R)-dihydroflavonol + succinate + CO2
-
-
?
a (2S)-flavan-4-one + 2-oxoglutarate + O2
Nymphaea hybrid cultivar
-
a (2R,3R)-dihydroflavonol + succinate + CO2
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
leaf
low expression level
Nymphaea hybrid cultivar
-
additional information
expression of F3H is specifically regulated in petals and stamens. The F3H expression is slightly diminished in petals of cultivar Satabankacha at the fully-opening stage, whereas it is detected in the cultivar Buntharik only when white petals are tinted with pink.
Nelumbo nucifera
-
petal
-
Nelumbo nucifera
-
petal
-
Nymphaea hybrid cultivar
-
stamen
-
Nelumbo nucifera
-
stamen
-
Nymphaea hybrid cultivar
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
a (2S)-flavan-4-one + 2-oxoglutarate + O2
-
744729
Nelumbo nucifera
a (2R,3R)-dihydroflavonol + succinate + CO2
-
-
-
?
a (2S)-flavan-4-one + 2-oxoglutarate + O2
-
744729
Nymphaea hybrid cultivar
a (2R,3R)-dihydroflavonol + succinate + CO2
-
-
-
?
General Information
General Information
Commentary
Organism
physiological function
F3H is a key regulator for the flavonoid biosynthesis pathway and plays a role in accumulation of anthocyanin pigments in the vacuole
Nelumbo nucifera
physiological function
F3H is a key regulator for the flavonoid biosynthesis pathway and plays a role in accumulation of anthocyanin pigments in the vacuole
Nymphaea hybrid cultivar
General Information (protein specific)
General Information
Commentary
Organism
physiological function
F3H is a key regulator for the flavonoid biosynthesis pathway and plays a role in accumulation of anthocyanin pigments in the vacuole
Nelumbo nucifera
physiological function
F3H is a key regulator for the flavonoid biosynthesis pathway and plays a role in accumulation of anthocyanin pigments in the vacuole
Nymphaea hybrid cultivar
Other publictions for EC 1.14.11.9
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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Isolation and expression anal ...
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Chiang Mai J. Sci.
44
427-437
2017
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2
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-
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4
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4
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4
1
1
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1
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1
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Pohlia nutans
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Molecular characterization of ...
Carthamus tinctorius
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1
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1
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Molecular cloning and identif ...
Lycium chinense
Plant Physiol. Biochem.
98
89-100
2016
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1
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2
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2
2
2
2
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1
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1
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3
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742549
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153
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5
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11
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13
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15
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5
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5
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743524
Li
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Viola x wittrockiana
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84
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Cloning and functional analys ...
Reaumuria trigyna
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36
1221-1229
2014
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1
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2
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3
6
2
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746091
Mahajan
Overexpression of a tea flava ...
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Plant Mol. Biol.
85
551-573
2014
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726211
Liu
Regulation of flavanone 3-hydr ...
Hololachna songarica
Plant Physiol. Biochem.
73C
161-167
2013
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726318
Cheng
Diversifying selection on flav ...
Glycine max, Glycine soja
PLoS ONE
8
e54154
2013
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4
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726200
Flachowsky
Silencing of flavanone-3-hydro ...
Malus domestica
Plant Physiol. Biochem.
51
18-25
2012
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1
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2
2
1
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725165
Himi
Differential expression of thr ...
Triticum aestivum
Int. J. Plant Genomics
2011
369460
2011
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3
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10
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3
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3
9
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713238
Lovdal
Synergetic effects of nitrogen ...
Solanum lycopersicum, Solanum lycopersicum Suzanne
Phytochemistry
71
605-613
2010
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1
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2
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1
1
1
1
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713256
Liu
Molecular dissection of the pa ...
Sorghum bicolor
Plant Cell Physiol.
51
1173-1185
2010
-
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1
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1
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1
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1
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1
3
3
1
-
-
698431
Halbwirth
Measuring flavonoid enzyme act ...
no activity in Actinidia deliciosa, Prunus avium, Prunus cerasus, Prunus domestica, Ribes uva-crispa, Rubus idaeus, Rubus plicatus, Sambucus nigra
J. Agric. Food Chem.
57
4983-4987
2009
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7
-
14
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9
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7
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22
28
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21
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21
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21
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21
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7
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14
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7
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22
28
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21
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-
-
700130
Cheng
-
Polymorphisms of soybean isofl ...
Glycine max, Glycine soja
Mol. Breed.
25
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2009
-
2
2
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2
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2
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2
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4
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2
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-
2
-
-
-
-
-
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-
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-
-
-
-
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-
700225
Khlestkina
Anthocyanin biosynthesis genes ...
Secale cereale
Mol. Genet. Genomics
282
475-485
2009
-
-
1
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-
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4
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1
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-
-
-
-
-
-
-
-
700742
Zheng
-
Sugars induce anthocyanin accu ...
Vitis vinifera
Plant Growth Regul.
58
251-260
2009
1
1
-
-
-
-
-
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1
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1
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1
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3
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1
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1
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1
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-
700794
Eungwanichayapant
Accumulation of catechins in t ...
Camellia sinensis var. sinensis
Plant Physiol. Biochem.
47
94-97
2009
-
1
-
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1
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2
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2
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1
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688942
Kim
Accumulation of flavonols in r ...
Glycine max
Mol. Cells
25
247-252
2008
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1
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1
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1
2
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1
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1
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4
1
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-
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-
-
-
-
688943
Kim
Flavanone 3beta-hydroxylases f ...
Oryza sativa
Mol. Cell
25
312-316
2008
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-
1
-
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1
3
-
-
3
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5
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1
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12
1
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1
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3
3
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3
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3
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3
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3
-
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12
3
-
-
-
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-
-
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689653
Owens
Biochemical and genetic charac ...
Arabidopsis thaliana
Plant Physiol. Biochem.
46
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2008
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1
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1
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4
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1
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1
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1
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1
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1
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1
-
1
-
1
-
1
-
1
-
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690168
Singh
An early gene of the flavonoid ...
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Tree Physiol.
28
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2008
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-
1
-
-
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3
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1
1
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1
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1
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1
1
-
1
-
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-
-
-
-
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-
-
-
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700308
Hukkanen
Benzothiadiazole affects the l ...
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Mol. Plant Pathol.
9
799-808
2008
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4
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1
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1
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-
-
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701213
Nakatsuka
-
Isolation and expression analy ...
Rhododendron x pulchrum
Sci. Hortic.
118
314-320
2008
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1
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4
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2
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1
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4
-
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-
-
-
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1
1
1
1
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746540
Singh
An early gene of the flavonoi ...
Camellia sinensis
Tree Physiol.
28
1349-1356
2008
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-
1
-
-
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-
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1
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3
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1
1
-
1
1
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1
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-
1
-
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1
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1
1
-
1
1
-
-
-
-
-
-
-
1
2
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-
2
-
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689577
Gebhardt
Evolution of flavone synthase ...
Petroselinum crispum
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144
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2007
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1
-
16
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4
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1
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1
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16
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1
-
-
-
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671524
Miyahisa
Combinatorial biosynthesis of ...
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1
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1
-
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-
-
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-
-
-
-
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-
-
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674117
Halbwirth
Two-phase flavonoid formation ...
Fragaria x ananassa
J. Agric. Food Chem.
54
1479-1485
2006
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-
-
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3
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1
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1
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1
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1
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2
-
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-
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-
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675792
Leonard
Functional expression of a P45 ...
Malus domestica
Metab. Eng.
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172-181
2006
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1
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1
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2
-
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-
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-
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-
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676671
Halbwirth
-
Screening for inhibitors of 2- ...
Malus domestica, Pyrus communis
Plant Sci.
171
194-205
2006
12
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2
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22
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2
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2
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4
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6
2
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2
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2
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-
-
12
-
2
2
-
-
-
-
22
-
-
-
2
-
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-
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-
-
2
-
-
4
-
2
-
-
-
2
-
-
-
-
-
-
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-
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658733
Wellmann
Significance of C-terminal seq ...
Petunia x hybrida
FEBS Lett.
561
149-154
2004
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1
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1
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4
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2
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1
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-
1
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1
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1
-
-
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4
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655572
Martens
Divergent evolution of flavono ...
Petroselinum crispum
FEBS Lett.
544
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1
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1
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660098
Pelt
Flavanone 3-hydroxylase expres ...
Citrus x paradisi, Petunia sp.
Phytochemistry
64
435-444
2003
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10
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439125
Lukacin
Site-directed mutagenesis of t ...
Petunia x hybrida
Eur. J. Biochem.
267
853-860
2000
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4
1
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1
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1
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439127
Lukacin
The monomeric polypeptide comp ...
Petunia x hybrida
FEBS Lett.
467
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2000
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Lukacin
Identification of strictly con ...
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439128
Pelletier
Analysis of flavanone 3-hydrox ...
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439122
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Molecular characterization and ...
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439126
Britsch
Molecular characterization of ...
Antirrhinum majus, Callistephus chinensis, Dianthus caryophyllus, Hordeum vulgare, Matthiola incana, Petunia x hybrida
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439113
Britsch
Purification and characterizat ...
Petroselinum crispum
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282
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1990
1
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3
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3
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1
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3
1
3
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1
1
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1
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1
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3
1
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1
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439114
Britsch
Purification of flavanone 3 be ...
Petunia x hybrida
Arch. Biochem. Biophys.
276
348-354
1990
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1
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1
-
-
-
-
-
-
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-
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-
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439123
O'Neill
Molecular genetic analysis of ...
Solanum lycopersicum
Mol. Gen. Genet.
224
279-288
1990
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1
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1
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1
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1
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1
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-
-
-
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-
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-
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-
439115
Beerhues
-
Flavanone 3-hydroxylase and di ...
Tulipa hybrid cultivar
J. Plant Physiol.
133
743-746
1989
-
-
-
-
-
-
4
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-
1
-
-
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
1
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-
-
-
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-
-
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-
-
-
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4
-
-
-
1
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-
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-
-
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1
-
-
2
-
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-
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1
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-
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-
439116
Takeda
-
Anthocyanin composition of Sin ...
Sinapis alba
Phytochemistry
27
1351-1353
1988
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-
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1
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1
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1
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1
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1
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1
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1
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439119
Britsch
Purification and characterizat ...
Petunia x hybrida
Eur. J. Biochem.
156
569-577
1986
2
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-
-
-
-
10
4
-
2
3
-
-
2
1
-
1
-
-
1
1
1
3
1
-
-
-
-
-
1
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-
-
2
-
-
2
-
-
-
-
-
-
-
10
2
4
-
2
3
-
-
1
-
1
-
1
1
1
3
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
439120
Forkmann
Selection and characterisation ...
Dahlia pinnata, Glandularia x hybrida, Streptocarpus hybrida, Zinnia elegans
Planta
161
261-265
1984
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-
-
-
-
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12
-
4
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-
4
-
7
-
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-
4
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4
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-
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-
-
-
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12
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4
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4
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4
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-
4
-
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-
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-
438998
Dangelmayr
-
Relationship between flower de ...
Matthiola incana
Z. Naturforsch. C
38
551-555
1983
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-
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2
-
1
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4
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2
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2
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4
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-
2
-
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-
-
-
-
439001
Forkmann
-
Genetic control of flavanone 3 ...
Antirrhinum majus
Z. Naturforsch. C
36
411-416
1981
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-
-
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1
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1
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1
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1
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1
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-
-
-
-
439006
Forkmann
-
Anthocyanin biosynthesis in fl ...
Matthiola incana
Z. Naturforsch. C
35
691-695
1980
1
-
-
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5
-
1
1
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1
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-
1
1
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2
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1
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5
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1
1
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1
1
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2
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