BRENDA - Enzyme Database
show all sequences of 1.14.11.9

Molecular characterization of flavanone 3-hydroxylase gene and flavonoid accumulation in two chemotyped safflower lines in response to methyl jasmonate stimulation

Tu, Y.; Liu, F.; Guo, D.; Fan, L.; Zhu, Z.; Xue, Y.; Gao, Y.; Guo, M.; BMC Plant Biol. 16, 132 (2016)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
gene CtF3H, DNA and amino acid sequence determination and analysis, phylogenic analysis, quantitative real-time PCR enzyme expression analysis, functional recombinant expression of MBP-tagged enzyme in Escherichia coli BL21(DE3)pLysS from vector pMAL-C5x leading to production of dihydrokaempferol when naringenin is available the substrate. Recombinant expression of GFP-tagged enzyme in onion cells via transfromation of Agrobacterium tumefaciens strain GV3101, where it is localized both in the nucleus and in the cytosol
Carthamus tinctorius
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Carthamus tinctorius
5829
-
additional information
subcellular localization study
Carthamus tinctorius
-
-
nucleus
-
Carthamus tinctorius
5634
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Fe2+
dependent on, required for catalysis, the ferrous iron binding site HxDxnH is formed by His78, His121, His211, Asp213, His221, Asp 223, His265, His267, and His279
Carthamus tinctorius
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
a (2S)-flavan-4-one + 2-oxoglutarate + O2
Carthamus tinctorius
-
a (2R,3R)-dihydroflavonol + succinate + CO2
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Carthamus tinctorius
M9MTF3
a quinochalcone-type safflower line with orange-yellow flowers and a flavonol-type safflower line with white flowers, i.e. ZHH0119 line and XHH007 line, respectively
-
Purification (Commentary)
Purification (Commentary)
Organism
recombinant MBP-tagged enzyme from Escherichia coli BL21(DE3)pLysS by amylose affinity chromatography
Carthamus tinctorius
Source Tissue
Source Tissue
Commentary
Organism
Textmining
flower
the transcriptome expression of CtF3H shows a diametrically opposed expression pattern in a quinochalcone-type safflower line (with orange-yellow flowers) and a flavonol-type safflower line (with white flowers) under external stimulation by methyl jasmonate (MeJA), which has been identified as an elicitor of flavonoid metabolites. High expression of CtF3H in quinochalcone-type safflower line
Carthamus tinctorius
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
a (2S)-flavan-4-one + 2-oxoglutarate + O2
-
744604
Carthamus tinctorius
a (2R,3R)-dihydroflavonol + succinate + CO2
-
-
-
?
a (2S)-flavan-4-one + 2-oxoglutarate + O2
the 2-oxoglutarate binding site RxS is formed by Arg289 and Ser291
744604
Carthamus tinctorius
a (2R,3R)-dihydroflavonol + succinate + CO2
-
-
-
?
additional information
no activity with kaempferol, dihydrokaempferol, quercetin, and dihydroquercetin
744604
Carthamus tinctorius
?
-
-
-
-
naringenin + 2-oxoglutarate + O2
-
744604
Carthamus tinctorius
dihydroquercetin + succinate + CO2
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 40720, sequence calculation, x * 40000, recombinant detagged enzyme, SDS-PAGE
Carthamus tinctorius
Synonyms
Synonyms
Commentary
Organism
CtF3H
-
Carthamus tinctorius
F3H
-
Carthamus tinctorius
flavanone 3-hydroxylase
-
Carthamus tinctorius
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Carthamus tinctorius
sequence calculation
-
5.57
Cloned(Commentary) (protein specific)
Commentary
Organism
gene CtF3H, DNA and amino acid sequence determination and analysis, phylogenic analysis, quantitative real-time PCR enzyme expression analysis, functional recombinant expression of MBP-tagged enzyme in Escherichia coli BL21(DE3)pLysS from vector pMAL-C5x leading to production of dihydrokaempferol when naringenin is available the substrate. Recombinant expression of GFP-tagged enzyme in onion cells via transfromation of Agrobacterium tumefaciens strain GV3101, where it is localized both in the nucleus and in the cytosol
Carthamus tinctorius
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Carthamus tinctorius
5829
-
additional information
subcellular localization study
Carthamus tinctorius
-
-
nucleus
-
Carthamus tinctorius
5634
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Fe2+
dependent on, required for catalysis, the ferrous iron binding site HxDxnH is formed by His78, His121, His211, Asp213, His221, Asp 223, His265, His267, and His279
Carthamus tinctorius
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
a (2S)-flavan-4-one + 2-oxoglutarate + O2
Carthamus tinctorius
-
a (2R,3R)-dihydroflavonol + succinate + CO2
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant MBP-tagged enzyme from Escherichia coli BL21(DE3)pLysS by amylose affinity chromatography
Carthamus tinctorius
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
flower
the transcriptome expression of CtF3H shows a diametrically opposed expression pattern in a quinochalcone-type safflower line (with orange-yellow flowers) and a flavonol-type safflower line (with white flowers) under external stimulation by methyl jasmonate (MeJA), which has been identified as an elicitor of flavonoid metabolites. High expression of CtF3H in quinochalcone-type safflower line
Carthamus tinctorius
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
a (2S)-flavan-4-one + 2-oxoglutarate + O2
-
744604
Carthamus tinctorius
a (2R,3R)-dihydroflavonol + succinate + CO2
-
-
-
?
a (2S)-flavan-4-one + 2-oxoglutarate + O2
the 2-oxoglutarate binding site RxS is formed by Arg289 and Ser291
744604
Carthamus tinctorius
a (2R,3R)-dihydroflavonol + succinate + CO2
-
-
-
?
additional information
no activity with kaempferol, dihydrokaempferol, quercetin, and dihydroquercetin
744604
Carthamus tinctorius
?
-
-
-
-
naringenin + 2-oxoglutarate + O2
-
744604
Carthamus tinctorius
dihydroquercetin + succinate + CO2
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 40720, sequence calculation, x * 40000, recombinant detagged enzyme, SDS-PAGE
Carthamus tinctorius
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Carthamus tinctorius
sequence calculation
-
5.57
Expression
Organism
Commentary
Expression
Carthamus tinctorius
the enzyme flavanone 3-hydroxylase is induced by methyl jasmonate. Further metabolite analysis shows the increasing tendency of quinochalcones and flavonols, such as hydroxysafflor yellow A, kaempferol-3-O-beta-D-glucoside, kaempferol-3-O-beta-rutinoside, rutin, carthamin, and luteolin, in the quinochalcone-type safflower line. Also, the accumulation of kaempferol-3-O-beta-rutinoside and kaempferol-3-O-beta-D-glucoside in flavonols-typed safflower line shows enhanced accumulation pattern after methyl jasmonate treatment. Other flavonols, such as kaempferol, dihydrokaempferol and quercetin-3-O-beta-D-glucoside, in flavonols-typed safflower line present downregulation in respons to the methyl jasmonate stimulus
up
General Information
General Information
Commentary
Organism
evolution
the enzyme belongs to the Fe2+/2–oxoglutarate-dependent dioxygenase superfamily
Carthamus tinctorius
physiological function
flavanone 3-hydroxylase (F3H) is one of the nuclear enzymes acting at the bifurcation of the flavonoid biosynthetic pathway, initiating catalysis of the 3-hydroxylation of (2S)-flavanones, such as naringenin to dihydroflavonols
Carthamus tinctorius
General Information (protein specific)
General Information
Commentary
Organism
evolution
the enzyme belongs to the Fe2+/2–oxoglutarate-dependent dioxygenase superfamily
Carthamus tinctorius
physiological function
flavanone 3-hydroxylase (F3H) is one of the nuclear enzymes acting at the bifurcation of the flavonoid biosynthetic pathway, initiating catalysis of the 3-hydroxylation of (2S)-flavanones, such as naringenin to dihydroflavonols
Carthamus tinctorius
Expression (protein specific)
Organism
Commentary
Expression
Carthamus tinctorius
the enzyme flavanone 3-hydroxylase is induced by methyl jasmonate. Further metabolite analysis shows the increasing tendency of quinochalcones and flavonols, such as hydroxysafflor yellow A, kaempferol-3-O-beta-D-glucoside, kaempferol-3-O-beta-rutinoside, rutin, carthamin, and luteolin, in the quinochalcone-type safflower line. Also, the accumulation of kaempferol-3-O-beta-rutinoside and kaempferol-3-O-beta-D-glucoside in flavonols-typed safflower line shows enhanced accumulation pattern after methyl jasmonate treatment. Other flavonols, such as kaempferol, dihydrokaempferol and quercetin-3-O-beta-D-glucoside, in flavonols-typed safflower line present downregulation in respons to the methyl jasmonate stimulus
up
Other publictions for EC
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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1
1
-
1
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1
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5
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3
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2
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2
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1
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3
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2
2
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744729
Chaipanya
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Isolation and expression anal ...
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44
427-437
2017
-
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2
-
2
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2
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2
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6
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2
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2
2
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745484
Zhang
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Molecular cloning and functio ...
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4
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1
1
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745623
Khumkarjorn
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Cloning and expression analys ...
x Ascocenda, x Ascocenda NK-2012
J. Plant Biochem. Biotechnol.
26
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2017
1
-
1
-
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1
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1
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4
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2
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2
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4
1
3
1
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1
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1
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1
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1
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1
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1
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1
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4
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2
-
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4
1
1
-
1
-
1
-
-
1
-
-
-
-
-
-
746071
Li
-
PnF3H, a flavanone 3-hydroxyl ...
Pohlia nutans
Plant Growth Regul.
83
489-500
2017
1
-
1
-
1
-
-
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-
1
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2
-
1
-
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3
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1
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2
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1
1
1
1
-
-
744604
Tu
Molecular characterization of ...
Carthamus tinctorius
BMC Plant Biol.
16
132
2016
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-
1
-
-
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3
1
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1
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6
-
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1
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1
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4
1
3
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1
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1
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3
1
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1
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1
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1
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4
1
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1
1
2
2
1
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-
746122
Xiong
Molecular cloning and charact ...
Artemisia annua
Plant Physiol. Biochem.
105
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2016
-
-
1
-
1
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2
-
1
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1
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2
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1
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1
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1
3
1
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1
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1
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1
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1
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1
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1
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1
1
1
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1
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1
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746138
Song
Molecular cloning and identif ...
Lycium chinense
Plant Physiol. Biochem.
98
89-100
2016
-
-
1
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2
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4
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6
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2
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2
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1
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2
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6
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2
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2
2
2
2
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745621
Kumar
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Functional characterization o ...
Phyllanthus emblica
J. Plant Biochem. Biotechnol.
24
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2015
1
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1
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1
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1
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1
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3
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3
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3
1
1
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1
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3
3
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742549
Flores
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Fragaria x ananassa, Ribes nigrum, Ribes rubrum, Rubus idaeus, Rubus plicatus, Vaccinium myrtillus
Food Chem.
153
130-133
2014
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5
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5
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8
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11
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15
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15
5
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5
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13
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15
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5
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5
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743524
Li
Flower color patterning in pa ...
Viola x wittrockiana
Plant Physiol. Biochem.
84
134-141
2014
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1
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743971
Zhang
-
Cloning and functional analys ...
Reaumuria trigyna
Acta Physiol. Plant.
36
1221-1229
2014
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1
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2
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6
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2
2
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1
3
6
2
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746091
Mahajan
Overexpression of a tea flava ...
Camellia sinensis, Camellia sinensis UPASI-10
Plant Mol. Biol.
85
551-573
2014
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1
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6
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1
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6
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1
1
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726211
Liu
Regulation of flavanone 3-hydr ...
Hololachna songarica
Plant Physiol. Biochem.
73C
161-167
2013
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1
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1
1
1
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4
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1
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1
1
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1
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1
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726318
Cheng
Diversifying selection on flav ...
Glycine max, Glycine soja
PLoS ONE
8
e54154
2013
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2
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4
4
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726200
Flachowsky
Silencing of flavanone-3-hydro ...
Malus domestica
Plant Physiol. Biochem.
51
18-25
2012
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1
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1
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1
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1
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2
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3
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3
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3
1
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1
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Molecular dissection of the pa ...
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3
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698431
Halbwirth
Measuring flavonoid enzyme act ...
no activity in Actinidia deliciosa, Prunus avium, Prunus cerasus, Prunus domestica, Ribes uva-crispa, Rubus idaeus, Rubus plicatus, Sambucus nigra
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700225
Khlestkina
Anthocyanin biosynthesis genes ...
Secale cereale
Mol. Genet. Genomics
282
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700742
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Sugars induce anthocyanin accu ...
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2009
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1
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700794
Eungwanichayapant
Accumulation of catechins in t ...
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688942
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688943
Kim
Flavanone 3beta-hydroxylases f ...
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Halbwirth
Two-phase flavonoid formation ...
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Leonard
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Halbwirth
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Screening for inhibitors of 2- ...
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439125
Lukacin
Site-directed mutagenesis of t ...
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Lukacin
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3
-
1
1
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-
1
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-
1
1
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-
1
-
3
1
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-
-
-
-
1
-
-
-
1
-
-
1
-
-
-
-
-
-
-
3
1
3
-
1
1
-
-
-
-
1
-
-
1
-
3
1
-
-
-
-
1
-
-
-
-
-
-
-
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-
439114
Britsch
Purification of flavanone 3 be ...
Petunia x hybrida
Arch. Biochem. Biophys.
276
348-354
1990
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1
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1
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1
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1
-
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1
-
-
-
-
-
-
-
-
-
-
-
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-
439123
O'Neill
Molecular genetic analysis of ...
Solanum lycopersicum
Mol. Gen. Genet.
224
279-288
1990
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-
-
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1
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1
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1
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1
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1
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1
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1
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-
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439115
Beerhues
-
Flavanone 3-hydroxylase and di ...
Tulipa hybrid cultivar
J. Plant Physiol.
133
743-746
1989
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-
-
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4
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1
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1
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1
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2
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1
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4
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1
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1
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2
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1
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-
439116
Takeda
-
Anthocyanin composition of Sin ...
Sinapis alba
Phytochemistry
27
1351-1353
1988
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1
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1
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1
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1
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1
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1
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1
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439119
Britsch
Purification and characterizat ...
Petunia x hybrida
Eur. J. Biochem.
156
569-577
1986
2
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-
-
-
-
10
4
-
2
3
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-
2
1
-
1
-
-
1
1
1
3
1
-
-
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-
-
1
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-
-
2
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2
-
-
-
-
-
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10
2
4
-
2
3
-
-
1
-
1
-
1
1
1
3
1
-
-
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1
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-
439120
Forkmann
Selection and characterisation ...
Dahlia pinnata, Glandularia x hybrida, Streptocarpus hybrida, Zinnia elegans
Planta
161
261-265
1984
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12
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4
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4
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7
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4
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4
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12
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4
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4
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4
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4
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-
438998
Dangelmayr
-
Relationship between flower de ...
Matthiola incana
Z. Naturforsch. C
38
551-555
1983
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2
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1
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4
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2
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2
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4
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2
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-
439001
Forkmann
-
Genetic control of flavanone 3 ...
Antirrhinum majus
Z. Naturforsch. C
36
411-416
1981
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-
-
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-
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1
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1
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1
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1
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1
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-
439006
Forkmann
-
Anthocyanin biosynthesis in fl ...
Matthiola incana
Z. Naturforsch. C
35
691-695
1980
1
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5
-
1
1
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1
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1
1
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2
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1
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5
-
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1
1
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1
1
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2
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