Application | Comment | Organism |
---|---|---|
synthesis | functional expression of plant-derived O-methyltransferase, flavanone 3-hydroxylase, and flavonol synthase in Corynebacterium glutamicum for production of pterostilbene, kaempferol, and quercetin | Petunia x hybrida |
synthesis | expression of heterologous dioxygenase genes in (2S)-flavanone-producing Corynebacterium glutamicum strains enables the production of flavanonols and flavonols, e.g kaempferol and quercetin, starting from thephenylpropanoids p-coumaric acid and caffeic acid | Petunia x hybrida |
Cloned (Comment) | Organism |
---|---|
functional expression of plant-derived O-methyltransferase, flavanone 3-hydroxylase, and flavonol synthase in Corynebacterium glutamicum for production of pterostilbene, kaempferol, and quercetin | Petunia x hybrida |
gene F3H, functional recombinant expression of codon-optimized enzyme in Corynebacterium glutamicum strain DelAro4, coexpression with gene FLS encoding flavonol synthase from Populus deltoides. Coexpression fo genes F3H and FLS both from Petunia hybrida is not successful | Petunia x hybrida |
Protein Variants | Comment | Organism |
---|---|---|
additional information | the enzyme is ngineered for synthesizing polyphenol core structuressuch as the stilbene resveratrol and the (2S)-flavanone naringenin | Petunia x hybrida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
a (2S)-flavan-4-one + 2-oxoglutarate + O2 | Petunia x hybrida | - |
a (2R,3R)-dihydroflavonol + succinate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Petunia x hybrida | O22530 | - |
- |
Petunia x hybrida | Q07512 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
a (2S)-flavan-4-one + 2-oxoglutarate + O2 | - |
Petunia x hybrida | a (2R,3R)-dihydroflavonol + succinate + CO2 | - |
? | |
eriodictyol + 2-oxoglutarate + O2 | - |
Petunia x hybrida | dihydroquercetin + succinate + CO2 | - |
? | |
naringenin + 2-oxoglutarate + O2 | - |
Petunia x hybrida | dihydrokaempferol + succinate + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
F3H | - |
Petunia x hybrida |
flavanone 3-hydroxylase | - |
Petunia x hybrida |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the family of 2-oxoglutarate-dependent dioxygenases | Petunia x hybrida |
metabolism | (2S)-flavanones are converted to flavonols by the activity of the 2-oxoglutarate-dependent dioxygenases flavanone 3-hydroxylase (F3H) and flavonol synthase (FLS) | Petunia x hybrida |