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Literature summary for 1.14.11.80 extracted from

  • Liu, M.; Denizio, J.; Kohli, R.
    Quantification of oxidized 5-methylcytosine bases and TET enzyme activity (2016), Methods Enzymol., 573, 365-385.
    View publication on PubMed
Search for more literature for 1.14.11.80

Cloned(Commentary)

Cloned (Comment) Organism
expressed in HEK-293T cells and Sf9 insect cells Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ 0.075 mM ammonium iron(II) sulfate is used in optimized assay conditions Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5-formylcytosine in DNA + 2-oxoglutarate + O2 Homo sapiens
-
 5-carboxycytosine in DNA + succinate + CO2
-
 ?
5-hydroxymethylcytosine in DNA + 2-oxoglutarate + O2 Homo sapiens
-
 5-formylcytosine in DNA + succinate + CO2 + H2O
-
 ?
5-methylcytosine in DNA + 2-oxoglutarate + O2 Homo sapiens
-
 5-hydroxymethylcytosine in DNA + succinate + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens O43151
-
-
Homo sapiens Q6N021
-
-
Homo sapiens Q8NFU7
-
-

Purification (Commentary)

Purification (Comment) Organism
anti-FLAG M2 affinity gel filtration Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-formylcytosine in DNA + 2-oxoglutarate + O2
-
 Homo sapiens 5-carboxycytosine in DNA + succinate + CO2
-
 ?
5-hydroxymethylcytosine in DNA + 2-oxoglutarate + O2
-
 Homo sapiens 5-formylcytosine in DNA + succinate + CO2 + H2O
-
 ?
5-methylcytosine in DNA + 2-oxoglutarate + O2
-
 Homo sapiens 5-hydroxymethylcytosine in DNA + succinate + CO2
-
 ?

Synonyms

Synonyms Comment Organism
Ten-eleven translocation
-
 Homo sapiens
TET1
-
 Homo sapiens
TET2
-
 Homo sapiens
TET3
-
 Homo sapiens

Cofactor

Cofactor Comment Organism Structure
2-oxoglutarate
-
 Homo sapiens
ascorbate
-
 Homo sapiens