Cloned (Comment) | Organism |
---|---|
when the putative cDNA is expressed in either Escherichia coli or Saccharomyces cerevisiae no enzyme activity can be detected in lysates of theses cells. High activity can be measured in lysates of COS cells transfected with the putative rat cDNA, whereas only low activity is measured in lysates of cells transfected with the pcDNA3 vector without insert | Homo sapiens |
when the putative cDNA is expressed in either Escherichia coli or Saccharomyces cerevisiae no enzyme activity can be detected in lysates of theses cells. High activity can be measured in lysates of COS cells transfected with the putative rat cDNA, whereas only low activity is measured in lysates of cells transfected with the pcDNA3 vector without insert | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.109 | - |
2-oxoglutarate | - |
Rattus norvegicus | |
1.1 | - |
N6,N6,N6-Trimethyl-L-lysine | pH 6.7, 37°C | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | exclusively in | Rattus norvegicus | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | Km: 0.054 mM | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
2 * 43000, calculation from nucleotide sequence | Rattus norvegicus |
87000 | - |
gel filtration, non-denaturing PAGE | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N6,N6,N6-trimethyl-L-lysine + 2-oxoglutarate + O2 | Rattus norvegicus | first enzyme of the L-carnitine biosynthesis | 3-hydroxy-N6,N6,N6-trimethyl-L-lysine + succinate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q9NVH6 | - |
- |
Mus musculus | Q91ZE0 | - |
- |
Rattus norvegicus | Q91ZW6 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | the enzyme is synthesized as a 47500 Da precursor and processed to a mature protein of 43000 Da, presumably upon import into mitochondria | Rattus norvegicus |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0586 | - |
- |
Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N6,N6,N6-trimethyl-L-lysine + 2-oxoglutarate + O2 | - |
Homo sapiens | 3-hydroxy-N6,N6,N6-trimethyl-L-lysine + succinate + CO2 | - |
? | |
N6,N6,N6-trimethyl-L-lysine + 2-oxoglutarate + O2 | - |
Rattus norvegicus | 3-hydroxy-N6,N6,N6-trimethyl-L-lysine + succinate + CO2 | - |
? | |
N6,N6,N6-trimethyl-L-lysine + 2-oxoglutarate + O2 | first enzyme of the L-carnitine biosynthesis | Rattus norvegicus | 3-hydroxy-N6,N6,N6-trimethyl-L-lysine + succinate + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 43000, calculation from nucleotide sequence | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
epsilon-N-trimethyllysine hydroxylase | - |
Homo sapiens |
epsilon-N-trimethyllysine hydroxylase | - |
Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7.5 | at 37°C | Rattus norvegicus |