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Literature summary for 1.14.11.77 extracted from
- Characterization of a sulfur-regulated oxygenative alkylsulfatase from Pseudomonas putida S-313 (2000), J. Biol. Chem., 275, 31661-31667 .
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| ascorbate | 0.2 mM, 3fold increase in activity | Pseudomonas putida |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Pseudomonas putida |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | 0.1 mM, complete loss of activity | Pseudomonas putida | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.034 | - |
Dodecyl sulfate | pH 7, 30°C | Pseudomonas putida | |
| 0.04 | - |
hexyl sulfate | pH 7, 30°C | Pseudomonas putida | |
| 0.06 | - |
nonyl sulfate | pH 7, 30°C | Pseudomonas putida | |
| 0.066 | - |
2-ethylhexyl sulfate | pH 7, 30°C | Pseudomonas putida | |
| 0.097 | - |
decyl sulfate | pH 7, 30°C | Pseudomonas putida | |
| 0.14 | - |
2-oxoglutarate | pH 7, 30°C | Pseudomonas putida | |
| 0.271 | - |
heptyl sulfate | pH 7, 30°C | Pseudomonas putida | |
| 0.437 | - |
octyl sulfate | pH 7, 30°C | Pseudomonas putida | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | required, maximum acitivy at 0.1 mM | Pseudomonas putida | |
| additional information | not activating: Ni2+, Co2+, Mn2+, Cu2+, Zn2+, Mg2+, and Ca2+ | Pseudomonas putida |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas putida | Q9WWU5 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant protein | Pseudomonas putida |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-ethylhexyl sulfate + 2-oxoglutarate + O2 | - |
Pseudomonas putida | 2-ethylhexanal + succinate + CO2 + sulfate | - |
? | |
| decyl sulfate + 2-oxoglutarate + O2 | - |
Pseudomonas putida | decanal + succinate + CO2 + sulfate | - |
? | |
| dodecyl sulfate + 2-oxoglutarate + O2 | - |
Pseudomonas putida | dodecanal + succinate + CO2 + sulfate | - |
? | |
| heptyl sulfate + 2-oxoglutarate + O2 | - |
Pseudomonas putida | heptanal + succinate + CO2 + sulfate | - |
? | |
| hexyl sulfate + 2-oxoglutarate + O2 | - |
Pseudomonas putida | hexanal + succinate + CO2 + sulfate | - |
? | |
| additional information | 2-keto acids support desulfation at the following rates, relative to the rate obtained with 2-oxoglutarate: 2-oxo-valerate 87%, 2-oxo-adipate 81%, 2-oxo-octanoate 31%, 3-methyl-2-oxo-butyrate 25%, and oxaloacetate 15%. No desulfation is obtained with pyruvate | Pseudomonas putida | ? | - |
- |
|
| nonyl sulfate + 2-oxoglutarate + O2 | - |
Pseudomonas putida | nonanal + succinate + CO2 + sulfate | - |
? | |
| octyl sulfate + 2-oxoglutarate + O2 | - |
Pseudomonas putida | octanal + succinate + CO2 + sulfate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 32000, SDS-PAGE | Pseudomonas putida |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alpha-ketoglutarate-dependent sulfate ester dioxygenase | - |
Pseudomonas putida |
| AtsK | - |
Pseudomonas putida |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 7.5 | - |
Pseudomonas putida |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | AtsK protein catalyzes the alpha-ketoglutarate-dependent cleavage of a range of alkyl sulfate esters, with chain lengths ranging from C4 to C12, requires oxygen and Fe2+ for activity and releases succinate, sulfate, and the corresponding aldehyde as products | Pseudomonas putida |
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