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Literature summary for 1.14.11.75 extracted from

  • Smirnov, S.; Sokolov, P.; Kotlyarova, V.; Samsonova, N.; Kodera, T.; Sugiyama, M.; Torii, T.; Hibi, M.; Shimizu, S.; Yokozeki, K.; Ogawa, J.
    A novel L-isoleucine-4'-dioxygenase and L-isoleucine dihydroxylation cascade in Pantoea ananatis (2013), MicrobiologyOpen, 2, 471-481.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.11.75

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Pantoea ananatis

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ dependent on Pantoea ananatis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
 x * 30000, SDS-PAGE Pantoea ananatis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(3S)-3'-hydroxy-L-isoleucine + 2-oxoglutarate + O2 Pantoea ananatis
-
 (3S,4R)-3',4-dihydroxy-L-isoleucine + succinate + CO2
-
 ?
(3S)-3'-hydroxy-L-isoleucine + 2-oxoglutarate + O2 Pantoea ananatis AJ13355
-
 (3S,4R)-3',4-dihydroxy-L-isoleucine + succinate + CO2
-
 ?
additional information Pantoea ananatis L-isoleucine 3'-hydroxylase HilA and 3'-hydroxy-L-isoleucine 4-hydroxylase HilB catalyze the consecutive hydroxylation of L-isoleucine at the C-3' and C-4 positions, respectively, to synthesize L-3',4-dihydroxyisoleucine. When expressed without HilA, HilB acts as a minimally effective L-isoleucine-4-dioxygenase ?
-
 ?
additional information Pantoea ananatis AJ13355 L-isoleucine 3'-hydroxylase HilA and 3'-hydroxy-L-isoleucine 4-hydroxylase HilB catalyze the consecutive hydroxylation of L-isoleucine at the C-3' and C-4 positions, respectively, to synthesize L-3',4-dihydroxyisoleucine. When expressed without HilA, HilB acts as a minimally effective L-isoleucine-4-dioxygenase ?
-
 ?

Organism

Organism UniProt Comment Textmining
Pantoea ananatis
-
-
-
Pantoea ananatis AJ13355
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(3S)-3'-hydroxy-L-isoleucine + 2-oxoglutarate + O2
-
 Pantoea ananatis (3S,4R)-3',4-dihydroxy-L-isoleucine + succinate + CO2
-
 ?
(3S)-3'-hydroxy-L-isoleucine + 2-oxoglutarate + O2
-
 Pantoea ananatis AJ13355 (3S,4R)-3',4-dihydroxy-L-isoleucine + succinate + CO2
-
 ?
additional information L-isoleucine 3'-hydroxylase HilA and 3'-hydroxy-L-isoleucine 4-hydroxylase HilB catalyze the consecutive hydroxylation of L-isoleucine at the C-3' and C-4 positions, respectively, to synthesize L-3',4-dihydroxyisoleucine. When expressed without HilA, HilB acts as a minimally effective L-isoleucine-4-dioxygenase Pantoea ananatis ?
-
 ?
additional information L-isoleucine 3'-hydroxylase HilA and 3'-hydroxy-L-isoleucine 4-hydroxylase HilB catalyze the consecutive hydroxylation of L-isoleucine at the C-3' and C-4 positions, respectively, to synthesize L-3',4-dihydroxyisoleucine. When expressed without HilA, HilB acts as a minimally effective L-isoleucine-4-dioxygenase Pantoea ananatis AJ13355 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 30000, SDS-PAGE Pantoea ananatis

Synonyms

Synonyms Comment Organism
HilB
-
 Pantoea ananatis
L-4'-hydroxyisoleucine, 2-oxoglutarate: oxygen oxidoreductase (4-hydroxylating)
-
 Pantoea ananatis
L-isoleucine-4'-dioxygenase
-
 Pantoea ananatis