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Literature summary for 1.14.11.72 extracted from

  • Rajakovich, L.; Pandelia, M.; Mitchell, A.; Chang, W.; Zhang, B.; Boal, A.; Krebs, C.; Bollinger, J.
    A new microbial pathway for organophosphonate degradation catalyzed by two previously misannotated non-heme-iron oxygenases (2019), Biochemistry, 58, 1627-1647 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.11.72

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Leisingera caerulea

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with Fe2+, [2-(trimethylamino)ethyl]phosphonate and 2-oxoglutarate, hanging drop vapor diffusion method, using 1.0-1.4 M Li2SO4 and 1.6-1.8 M (NH4)2SO4 Leisingera caerulea

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ dependent on Leisingera caerulea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
 gel filtration Leisingera caerulea

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
[2-(trimethylamino)ethyl]phosphonate + 2-oxoglutarate + O2 Leisingera caerulea
-
 [(1R)-1-hydroxy-2-(trimethylamino)ethyl]phosphonate + succinate + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Leisingera caerulea A0A4V8H042
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and Sephadex S-200 gel filtration Leisingera caerulea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-aminoethylphosphonate + 2-oxoglutarate + O2
-
 Leisingera caerulea ?
-
 ?
3-aminopropylphosphonate + 2-oxoglutarate + O2
-
 Leisingera caerulea ?
-
 ?
additional information no activity with gamma-butyrobetaine or analogue substrates Leisingera caerulea ?
-
-
phosphocholine + 2-oxoglutarate + O2
-
 Leisingera caerulea glycine betaine aldehyde + phosphate + succinate + CO2
-
 ?
[2-(dimethylamino)ethyl]phosphonate + 2-oxoglutarate + O2
-
 Leisingera caerulea [(1R)-1-hydroxy-2-(dimethylamino)ethyl]phosphonate + 2-oxoglutarate + O2
-
 ?
[2-(trimethylamino)ethyl]phosphonate + 2-oxoglutarate + O2
-
 Leisingera caerulea [(1R)-1-hydroxy-2-(trimethylamino)ethyl]phosphonate + succinate + CO2
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 41000, calculated from amino acid sequence Leisingera caerulea

Synonyms

Synonyms Comment Organism
gamma-butyrobetaine hydroxylase misannotation Leisingera caerulea
tmpA
-
 Leisingera caerulea

Cofactor

Cofactor Comment Organism Structure
2-oxoglutarate dependent on Leisingera caerulea