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Literature summary for 1.14.11.71 extracted from

  • Gama, S.; Vogt, M.; Kalina, T.; Hupp, K.; Hammerschmidt, F.; Pallitsch, K.; Zechel, D.
    An oxidative pathway for microbial utilization of methylphosphonic acid as a phosphate source (2019), ACS Chem. Biol., 14, 735-741 .
    View publication on PubMed
Search for more literature for 1.14.11.71

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Gimesia maris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.011
-
 2-oxoglutarate at pH 7.0 and 25°C Gimesia maris
1.5
-
 methylphosphonate at pH 7.0 and 25°C Gimesia maris

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ required for activity, Km value of 0.004 mM Gimesia maris

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
methylphosphonate + 2-oxoglutarate + O2 Gimesia maris
-
 hydroxymethylphosphonate + succinate + CO2
-
 ?
methylphosphonate + 2-oxoglutarate + O2 Gimesia maris DSM 8797
-
 hydroxymethylphosphonate + succinate + CO2
-
 ?
additional information Gimesia maris 2-aminoethylphosphonic acid, ethylphosphonic acid, aminomethylphosphonic acid, phosphonoalanine and glyphosate are not substrates ?
-
-
additional information Gimesia maris DSM 8797 2-aminoethylphosphonic acid, ethylphosphonic acid, aminomethylphosphonic acid, phosphonoalanine and glyphosate are not substrates ?
-
-

Organism

Organism UniProt Comment Textmining
Gimesia maris
-
-
-
Gimesia maris DSM 8797
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Gimesia maris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methylphosphonate + 2-oxoglutarate + O2
-
 Gimesia maris hydroxymethylphosphonate + succinate + CO2
-
 ?
methylphosphonate + 2-oxoglutarate + O2
-
 Gimesia maris DSM 8797 hydroxymethylphosphonate + succinate + CO2
-
 ?
additional information 2-aminoethylphosphonic acid, ethylphosphonic acid, aminomethylphosphonic acid, phosphonoalanine and glyphosate are not substrates Gimesia maris ?
-
-
additional information 2-aminoethylphosphonic acid, ethylphosphonic acid, aminomethylphosphonic acid, phosphonoalanine and glyphosate are not substrates Gimesia maris DSM 8797 ?
-
-

Synonyms

Synonyms Comment Organism
phnY*
-
 Gimesia maris

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.36
-
 methylphosphonate at pH 7.0 and 25°C Gimesia maris
0.42
-
 2-oxoglutarate at pH 7.0 and 25°C Gimesia maris

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.24
-
 methylphosphonate at pH 7.0 and 25°C Gimesia maris
80
-
 2-oxoglutarate at pH 7.0 and 25°C Gimesia maris