Cloned (Comment) | Organism |
---|---|
gene KDM4A, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
purified recombinant enzyme in complex with substrate peptides, by vapour diffusion at 4°C from 0.1 M citrate, pH 5.5, 20% PEG 3350 and 4 mM NiCl2, X-ray diffraction structure determination and analysis at 2.1 A resolution | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | transposition of the Ala-Pro motif of H3K27 to AARK(me3)SPAAT, to mimic the proline position in H3K36, resulted in no detectable activity | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | structures of JMJD2A-Ni(II)-Zn(II) inhibitor complexes bound to tri-, di- and monomethyl forms of H3K9 and the trimethyl form of H3K36, overview | Homo sapiens | |
Ni2+ | - |
Homo sapiens | |
Zn2+ | - |
Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
nucleus | - |
Homo sapiens | 5634 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | the mechanism for achieving methylation state selectivity involves the orientation of the substrate methyl groups towards a ferryl intermediate | Homo sapiens | |
additional information | structures reveal a lysyl-binding pocket in which substrates are bound in distinct bent conformations involving the Zn-binding site | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
[histone H3]-N6,N6,N6-trimethyl-L-lysine 36 + 2-oxoglutarate + O2 | Homo sapiens | - |
[histone H3]-N6,N6-dimethyl-L-lysine 36 + succinate + formaldehyde + CO2 | - |
? | |
[histone H3]-N6,N6-dimethyl-L-lysine 36 + 2-oxoglutarate + O2 | Homo sapiens | - |
[histone H3]-N6-methyl-L-lysine 36 + succinate + formaldehyde + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | O75164 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally His-tagged enzyme from Escherichia coli by nickel affinity chromatography, gel filtration, and anion exchange chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | human enzyme JMJD2A (jumonji domain containing 2A) is selective towards tri- and dimethylated histone H3 lysyl residues 9 and 36 (H3K9me3/me2 and H3K36me3/me2), it discriminates between methylation states and achieves sequence selectivity for H3K9. Structures reveal a lysyl-binding pocket in which substrates are bound in distinct bent conformations involving the Zn2+-binding site | Homo sapiens | ? | - |
? | |
[histone H3]-N6,N6,N6-trimethyl-L-lysine 36 + 2-oxoglutarate + O2 | - |
Homo sapiens | [histone H3]-N6,N6-dimethyl-L-lysine 36 + succinate + formaldehyde + CO2 | - |
? | |
[histone H3]-N6,N6-dimethyl-L-lysine 36 + 2-oxoglutarate + O2 | - |
Homo sapiens | [histone H3]-N6-methyl-L-lysine 36 + succinate + formaldehyde + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
histone demethylase JmjD2A | - |
Homo sapiens |
JMJD2A | - |
Homo sapiens |
jumonji domain containing 2A | - |
Homo sapiens |
KDM4A | - |
Homo sapiens |
More | see also EC 1.14.11.66 | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
additional information | the mechanism for achieving methylation state selectivity involves the orientation of the substrate methyl groups towards a ferryl intermediate. Active site structure and mechanism of JMJD2A, overview | Homo sapiens |