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Literature summary for 1.14.11.69 extracted from

  • Strobl-Mazzulla, P.H.; Sauka-Spengler, T.; Bronner-Fraser, M.
    Histone demethylase JmjD2A regulates neural crest specification (2010), Dev. Cell, 19, 460-468 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene KDM4A, recombinant expression of GFP-tagged enzyme-deficient mutant and of point mutants in fibroblasts, overexpression of JmjD2A under the control beta-actin promoter in chicken fibroblasts specifically depletes H3K9me3 and H3K36me3 Gallus gallus

Protein Variants

Protein Variants Comment Organism
G133A site-directed mutagenesis, the mutation in the catalytic domain abrogates enzyme activity Gallus gallus
G138A site-directed mutagenesis, the mutation in the catalytic domain abrogates enzyme activity Gallus gallus
G165A site-directed mutagenesis, the mutation in the catalytic domain abrogates enzyme activity Gallus gallus
G170A site-directed mutagenesis, the mutation in the catalytic domain abrogates enzyme activity Gallus gallus
additional information overexpression of JmjD2A in fibroblasts specifically depletes H3K9me3 and H3K36me3 Gallus gallus
S288N site-directed mutagenesis, the mutation in the catalytic domain abrogates enzyme activity Gallus gallus
T289B site-directed mutagenesis, the mutation in the catalytic domain abrogates enzyme activity Gallus gallus

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleus
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Gallus gallus 5634
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Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Gallus gallus JmjD2A is specific for H3K9me3 and H3K36me3 substrates. JmjD2A directly binds to regulatory regions of neural crest specifier genes in vivo ?
-
?
[histone H3]-N6,N6,N6-trimethyl-L-lysine 36 + 2-oxoglutarate + O2 Gallus gallus
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[histone H3]-N6,N6-dimethyl-L-lysine 36 + succinate + formaldehyde + CO2
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?

Organism

Organism UniProt Comment Textmining
Gallus gallus E1C6D5
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-

Source Tissue

Source Tissue Comment Organism Textmining
embryo neural folds Gallus gallus
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neural crest JmjD2A transcripts are observed in the neural plate, neural plate border, and non-neural ectoderm, highest expression of JmjD2A at stage 5, decreasing from stage 8 onward Gallus gallus
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information JmjD2A is specific for H3K9me3 and H3K36me3 substrates. JmjD2A directly binds to regulatory regions of neural crest specifier genes in vivo Gallus gallus ?
-
?
additional information JmjD2A is specific for H3K9me3 and H3K36me3 substrates Gallus gallus ?
-
?
[histone H3]-N6,N6,N6-trimethyl-L-lysine 36 + 2-oxoglutarate + O2
-
Gallus gallus [histone H3]-N6,N6-dimethyl-L-lysine 36 + succinate + formaldehyde + CO2
-
?

Synonyms

Synonyms Comment Organism
JMJD2A
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Gallus gallus
JumonjiD2A
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Gallus gallus
KDM4A
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Gallus gallus
More see also EC 1.14.11.66 Gallus gallus

General Information

General Information Comment Organism
malfunction loss of JmjD2A function causes dramatic downregulation of neural crest specifier genes analyzed by multiplex NanoString and in situ hybridization. Cells overexpressing JmjD2A completely lack either H3K9me3 or H3K36me3 marks. Overexpression of JmjD2A in chicken fibroblasts specifically depletes H3K9me3 and H3K36me3. JmjD2A loss of function depletes Sox10 expression and expression of neural crest specifier genes Sox9, FoxD3, and Snail2, but not dorsal neural tube markers. JmjD2A knockdown inhibits demethylation of H3K9me3 on the Sox10 promoter Gallus gallus
physiological function histone demethylases such as members of the Jumonji family revert histone trimethylation. Unlike other demethylases, JmjD2/KDM4 proteins have been shown to remove both lysine 9 and 36 trimethyl marks. Dynamic histone modifications are critical for proper temporal control of neural crest gene expression in vivo. The histone demethylase, JumonjiD2A (JmjD2A/KDM4A), is expressed in the forming neural folds. Direct stage-specific interactions of JmjD2A with regulatory regions of neural crest genes, associated temporal modifications in methylation states of lysine residues are directly affected by JmjD2A activity. Chromatin modifications directly control neural crest genes in vertebrate embryos via modulating histone methylation. JmjD2A plays an important role in neural crest development. H3K9me3 and H3K36me3 occupancy regulates neural crest specifier expression in vivo Gallus gallus