Activating Compound | Comment | Organism | Structure |
---|---|---|---|
histone H3 | the in vitro demethylase activity of the enzyme is allosterically enhanced by binding of its product, unmodified histone H3 peptides, to its PHD1 reader domain | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expressed in Sf21 insect cells | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0168 | - |
[acetylated histone H3 21mer]-N6,N6,N6-trimethyl-L-lysine4 | in the presence of 0.038 mM unmodified histone H3 10mer peptide, at pH 7.5 and 25°C | Homo sapiens | |
0.0547 | - |
[acetylated histone H3 21mer]-N6,N6-dimethyl-L-lysine4 | in the presence of 0.038 mM unmodified histone H3 10mer peptide, at pH 7.5 and 25°C | Homo sapiens | |
0.0955 | - |
[acetylated histone H3 21mer]-N6,N6,N6-trimethyl-L-lysine4 | in the absence of unmodified histone H3 10mer peptide, at pH 7.5 and 25°C | Homo sapiens | |
0.229 | - |
[acetylated histone H3 21mer]-N6,N6-dimethyl-L-lysine4 | in the absence of unmodified histone H3 10mer peptide, at pH 7.5 and 25°C | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | 0.05 mM used in assay conditions | Homo sapiens | |
K+ | 50 mM used in assay conditions | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
[histone H3]-N6,N6,N6-trimethyl-L-lysine4 + 2-oxoglutarate + O2 | Homo sapiens | - |
[histone H3]-N6,N6-dimethyl-L-lysine4 + succinate + formaldehyde + CO2 | - |
? | |
[histone H3]-N6,N6,N6-trimethyl-L-lysine4 + 3 2-oxoglutarate + 3 O2 | Homo sapiens | overall reaction | [histone H3]-L-lysine4 + 3 succinate + 3 formaldehyde + 3 CO2 | - |
? | |
[histone H3]-N6,N6-dimethyl-L-lysine4 + 2-oxoglutarate + O2 | Homo sapiens | - |
[histone H3]-N6-methyl-L-lysine4 + succinate + formaldehyde + CO2 | - |
? | |
[histone H3]-N6-methyl-L-lysine4 + 2-oxoglutarate + O2 | Homo sapiens | - |
[histone H3]-L-lysine4 + succinate + formaldehyde + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
cobalt resin column chromatography and Superdex S200 gel filtration | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
[acetylated histone H3 21mer]-N6,N6,N6-trimethyl-L-lysine4 + 2-oxoglutarate + O2 | - |
Homo sapiens | [acetylated histone H3 21mer]-N6,N6-dimethyl-L-lysine4 + succinate + formaldehyde + CO2 | - |
? | |
[acetylated histone H3 21mer]-N6,N6-dimethyl-L-lysine4 + 2-oxoglutarate + O2 | - |
Homo sapiens | [acetylated histone H3 21mer]-N6-methyl-L-lysine4 + succinate + formaldehyde + CO2 | - |
? | |
[histone H3]-N6,N6,N6-trimethyl-L-lysine4 + 2-oxoglutarate + O2 | - |
Homo sapiens | [histone H3]-N6,N6-dimethyl-L-lysine4 + succinate + formaldehyde + CO2 | - |
? | |
[histone H3]-N6,N6,N6-trimethyl-L-lysine4 + 3 2-oxoglutarate + 3 O2 | overall reaction | Homo sapiens | [histone H3]-L-lysine4 + 3 succinate + 3 formaldehyde + 3 CO2 | - |
? | |
[histone H3]-N6,N6-dimethyl-L-lysine4 + 2-oxoglutarate + O2 | - |
Homo sapiens | [histone H3]-N6-methyl-L-lysine4 + succinate + formaldehyde + CO2 | - |
? | |
[histone H3]-N6-methyl-L-lysine4 + 2-oxoglutarate + O2 | - |
Homo sapiens | [histone H3]-L-lysine4 + succinate + formaldehyde + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
KDM5A | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.023 | - |
[acetylated histone H3 21mer]-N6,N6-dimethyl-L-lysine4 | in the absence of unmodified histone H3 10mer peptide, at pH 7.5 and 25°C | Homo sapiens | |
0.023 | - |
[acetylated histone H3 21mer]-N6,N6-dimethyl-L-lysine4 | in the presence of 0.038 mM unmodified histone H3 10mer peptide, at pH 7.5 and 25°C | Homo sapiens | |
0.041 | - |
[acetylated histone H3 21mer]-N6,N6,N6-trimethyl-L-lysine4 | in the presence of 0.038 mM unmodified histone H3 10mer peptide, at pH 7.5 and 25°C | Homo sapiens | |
0.047 | - |
[acetylated histone H3 21mer]-N6,N6,N6-trimethyl-L-lysine4 | in the absence of unmodified histone H3 10mer peptide, at pH 7.5 and 25°C | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.098 | - |
[acetylated histone H3 21mer]-N6,N6-dimethyl-L-lysine4 | in the absence of unmodified histone H3 10mer peptide, at pH 7.5 and 25°C | Homo sapiens | |
0.43 | - |
[acetylated histone H3 21mer]-N6,N6-dimethyl-L-lysine4 | in the presence of 0.038 mM unmodified histone H3 10mer peptide, at pH 7.5 and 25°C | Homo sapiens | |
0.49 | - |
[acetylated histone H3 21mer]-N6,N6,N6-trimethyl-L-lysine4 | in the absence of unmodified histone H3 10mer peptide, at pH 7.5 and 25°C | Homo sapiens | |
2.43 | - |
[acetylated histone H3 21mer]-N6,N6,N6-trimethyl-L-lysine4 | in the presence of 0.038 mM unmodified histone H3 10mer peptide, at pH 7.5 and 25°C | Homo sapiens |