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Literature summary for 1.14.11.66 extracted from

  • Ponnaluri, V.; Vavilala, D.; Putty, S.; Gutheil, W.; Mukherji, M.
    Identification of non-histone substrates for JMJD2A-C histone demethylases (2009), Biochem. Biophys. Res. Commun., 390, 280-284 .
    View publication on PubMed
Search for more literature for 1.14.11.66

Activating Compound

Activating Compound Comment Organism Structure
ascorbate
-
 Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
gene KDM4A, recombinant expression of GST-tagged enzyme in Escherichia coli strain BL21(DE3) Homo sapiens
gene KDM4B, recombinant expression of GST-tagged enzyme in Escherichia coli strain BL21(DE3) Homo sapiens
gene KDM4C, recombinant expression of GST-tagged enzyme in Escherichia coli strain BL21(DE3) Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
 Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ required for catalysis Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
[histone H3]-N6,N6,N6-trimethyl-L-lysine 9 + 2-oxoglutarate + O2 Homo sapiens
-
 [histone H3]-N6,N6-dimethyl-L-lysine 9 + succinate + formaldehyde + CO2
-
 ?
[histone H3]-N6,N6-dimethyl-L-lysine 9 + 2-oxoglutarate + O2 Homo sapiens
-
 [histone H3]-N6-methyl-L-lysine 9 + succinate + formaldehyde + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens O75164
-
-
Homo sapiens O94953
-
-
Homo sapiens Q9H3R0
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant GST-tagged enzyme from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography and dialysis Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00029
-
 recombinant isozyme JMJD2B, pH 7.5, 22°C, substrate H3K9me3 Homo sapiens
0.0004
-
 recombinant isozyme JMJD2B, pH 7.5, 22°C, substrate CSB-K297me3 Homo sapiens
0.0005
-
 recombinant isozyme JMJD2C, pH 7.5, 22°C, substrate H3K9me3 Homo sapiens
0.0006
-
 recombinant isozyme JMJD2A, pH 7.5, 22°C, substrate CSB-K448me3 Homo sapiens
0.00064
-
 recombinant isozyme JMJD2A, pH 7.5, 22°C, substrate CSB-K297me3 Homo sapiens
0.00095
-
 recombinant isozyme JMJD2B, pH 7.5, 22°C, substrate CDYL1-K135me3 Homo sapiens
0.001
-
 recombinant isozyme JMJD2A, pH 7.5, 22°C, substrate CDYL1-K135me3 Homo sapiens
0.001
-
 recombinant isozyme JMJD2A, pH 7.5, 22°C, substrate H3K9me3 Homo sapiens
0.0011
-
 recombinant isozyme JMJD2A, pH 7.5, 22°C, substrate CSB-K170me3 Homo sapiens
0.00143
-
 recombinant isozyme JMJD2B, pH 7.5, 22°C, substrate CSB-K1054me3 Homo sapiens
0.0015
-
 recombinant isozyme JMJD2C, pH 7.5, 22°C, substrate CSB-K297me3 Homo sapiens
0.00163
-
 recombinant isozyme JMJD2C, pH 7.5, 22°C, substrate CSB-K1054me3 Homo sapiens
0.00188
-
 recombinant isozyme JMJD2A, pH 7.5, 22°C, substrate WIZ-K305me3 Homo sapiens
0.0019
-
 recombinant isozyme JMJD2A, pH 7.5, 22°C, substrate CSB-K1054me3 Homo sapiens
0.0019
-
 recombinant isozyme JMJD2C, pH 7.5, 22°C, substrate CSB-K170me3 Homo sapiens
0.002
-
 recombinant isozyme JMJD2B, pH 7.5, 22°C, substrate CSB-K170me3 Homo sapiens
0.00212
-
 recombinant isozyme JMJD2C, pH 7.5, 22°C, substrate G9a-K185me3 Homo sapiens
0.00232
-
 recombinant isozyme JMJD2C, pH 7.5, 22°C, substrate CSB-K448me3 Homo sapiens
0.0024
-
 recombinant isozyme JMJD2B, pH 7.5, 22°C, substrate G9a-K185me3 Homo sapiens
0.0025
-
 recombinant isozyme JMJD2B, pH 7.5, 22°C, substrate CSB-K448me3 Homo sapiens
0.0026
-
 recombinant isozyme JMJD2A, pH 7.5, 22°C, substrate G9a-K185me3 Homo sapiens
0.0031
-
 recombinant isozyme JMJD2C, pH 7.5, 22°C, substrate WIZ-K305me3 Homo sapiens
0.0034
-
 recombinant isozyme JMJD2C, pH 7.5, 22°C, substrate CDYL1-K135me3 Homo sapiens
0.0035
-
 recombinant isozyme JMJD2B, pH 7.5, 22°C, substrate WIZ-K305me3 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CDYL1-K135me3 + 2-oxoglutarate + O2 from chromodomain Y-like protein Homo sapiens CDYL1-K135me2 + succinate + formaldehyde + CO2
-
 ?
CSB-K1054me3 + 2-oxoglutarate + O2 CBS is the Cockayne syndrome group B protein Homo sapiens CSB-K1054me2 + succinate + formaldehyde + CO2
-
 ?
CSB-K170me3 + 2-oxoglutarate + O2 CBS is the Cockayne syndrome group B protein Homo sapiens CSB-K170me2 + succinate + formaldehyde + CO2
-
 ?
CSB-K297me3 + 2-oxoglutarate + O2 CBS is the Cockayne syndrome group B protein Homo sapiens CSB-K297me2 + succinate + formaldehyde + CO2
-
 ?
CSB-K448me3 + 2-oxoglutarate + O2 CBS is the Cockayne syndrome group B protein Homo sapiens CSB-K448me2 + succinate + formaldehyde + CO2
-
 ?
G9a-K185me3 + 2-oxoglutarate + O2
-
 Homo sapiens G9a-K185me2 + succinate + formaldehyde + CO2
-
 ?
additional information the bifunctional enzyme is active on H3K9me3/me2 and H3K36me3/me2 (EC 1.14.11.69) substrates, and also on non-histone substrates, substrate specificities of isozymes JMJD2A-C, overview Homo sapiens ?
-
 ?
WIZ-K305me3 + 2-oxoglutarate + O2 from widely interspaced zinc finger motifs protein Homo sapiens WIZ-K305me2 + succinate + formaldehyde + CO2
-
 ?
[histone H3]-N6,N6,N6-trimethyl-L-lysine 9 + 2-oxoglutarate + O2
-
 Homo sapiens [histone H3]-N6,N6-dimethyl-L-lysine 9 + succinate + formaldehyde + CO2
-
 ?
[histone H3]-N6,N6-dimethyl-L-lysine 9 + 2-oxoglutarate + O2
-
 Homo sapiens [histone H3]-N6-methyl-L-lysine 9 + succinate + formaldehyde + CO2
-
 ?

Synonyms

Synonyms Comment Organism
histone demethylase
-
 Homo sapiens
JMJD2A
-
 Homo sapiens
JMJD2B
-
 Homo sapiens
JMJD2C
-
 Homo sapiens
KDM4A
-
 Homo sapiens
KDM4B
-
 Homo sapiens
Kdm4c
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Homo sapiens