Cloned (Comment) | Organism |
---|---|
gene jmj25, promoter-GUS fusion analysis and histochemical assay of GUS activity of wild-type and mutants | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | homozygous mutant ibm1-4 (SALK_035608) is identified by PCR-based genotyping. The jmj24/ibm1 double mutant is generated by crossing jmj24-3 to ibm1-4. jmj24-2 is in the Wassilewskija background, whereas jmj24-3 and ibm1-4 are in the Columbia background | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
histone H3 N6,N6-dimethyl-L-lysine9 + 2-oxoglutarate + O2 | Arabidopsis thaliana | - |
histone H3 N6-methyl-L-lysine9 + succinate + formaldehyde + CO2 | - |
? | |
histone H3 N6,N6-dimethyl-L-lysine9 + 2-oxoglutarate + O2 | Arabidopsis thaliana Col-0 | - |
histone H3 N6-methyl-L-lysine9 + succinate + formaldehyde + CO2 | - |
? | |
[histone H3]-N6,N6-dimethyl-L-lysine9 + 2 2-oxoglutarate + 2 O2 | Arabidopsis thaliana | overall reaction | [histone H3]-L-lysine9 + 2 succinate + 2 formaldehyde + 2 CO2 | - |
? | |
[histone H3]-N6,N6-dimethyl-L-lysine9 + 2-oxoglutarate + O2 | Arabidopsis thaliana | - |
[histone H3]-N6-methyl-L-lysine9 + succinate + formaldehyde + CO2 | - |
? | |
[histone H3]-N6-methyl-L-lysine9 + 2-oxoglutarate + O2 | Arabidopsis thaliana | - |
[histone H3]-L-lysine9 + succinate + formaldehyde + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Arabidopsis thaliana | Q9SSE9 | - |
- |
Arabidopsis thaliana Col-0 | Q9SSE9 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
histone H3 N6,N6-dimethyl-L-lysine9 + 2-oxoglutarate + O2 | - |
Arabidopsis thaliana | histone H3 N6-methyl-L-lysine9 + succinate + formaldehyde + CO2 | - |
? | |
histone H3 N6,N6-dimethyl-L-lysine9 + 2-oxoglutarate + O2 | - |
Arabidopsis thaliana Col-0 | histone H3 N6-methyl-L-lysine9 + succinate + formaldehyde + CO2 | - |
? | |
[histone H3]-N6,N6-dimethyl-L-lysine9 + 2 2-oxoglutarate + 2 O2 | overall reaction | Arabidopsis thaliana | [histone H3]-L-lysine9 + 2 succinate + 2 formaldehyde + 2 CO2 | - |
? | |
[histone H3]-N6,N6-dimethyl-L-lysine9 + 2-oxoglutarate + O2 | - |
Arabidopsis thaliana | [histone H3]-N6-methyl-L-lysine9 + succinate + formaldehyde + CO2 | - |
? | |
[histone H3]-N6-methyl-L-lysine9 + 2-oxoglutarate + O2 | - |
Arabidopsis thaliana | [histone H3]-L-lysine9 + succinate + formaldehyde + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
IBM1 | - |
Arabidopsis thaliana |
JMJ25 | - |
Arabidopsis thaliana |
jmjC histone demethylase | - |
Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
malfunction | The jmj24-3 mutation can partially complement ibm1 for phenotypic and gene expression defects | Arabidopsis thaliana |
malfunction | an enzyme mutation causes a very severe growth phenotype with a much reduced plant size | Arabidopsis thaliana |
physiological function | dimethylation of histone H3 lysine 9 (H3K9me2) is a heterochromatic mark linked to DNA methylation and gene repression. Removal of H3K9me2 from gene bodies by the jmjC histone demethylase JMJ25 inhibits DNA methylation and derepresses gene expression. JmjC domain protein JMJ24 antagonizes histone H3K9 demethylase IBM1/JMJ25 function and interacts with RNAi pathways for gene silencing | Arabidopsis thaliana |