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Literature summary for 1.14.11.65 extracted from

  • Maina, P.K.; Shao, P.; Jia, X.; Liu, Q.; Umesalma, S.; Marin, M.; Long, D.; Concepcion-Roman, S.; Qi, H.H.
    Histone demethylase PHF8 regulates hypoxia signaling through HIF1alpha and H3K4me3 (2017), Biochim. Biophys. Acta, 1860, 1002-1012 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.11.65

Protein Variants

Protein Variants Comment Organism
F279S site-directed mutagenesis Homo sapiens
additional information knockdown or knockout of PHF8 in 293T cells by RNAi or CRISPR-Cas9 system. The small guide RNAs (sgRNAs) target exon 8, which encodes amino acids 262-315 of the JmjC domain, and abolish PHF8 expression Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleus
-
 Homo sapiens 5634
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
[histone H3]-N6,N6-dimethyl-L-lysine 9 + 2-oxoglutarate + O2 Homo sapiens
-
 [histone H3]-N6-methyl-L-lysine 9 + succinate + formaldehyde + CO2
-
 ?
[histone H3]-N6-methyl-L-lysine 9 + 2-oxoglutarate + O2 Homo sapiens
-
 [histone H3]-L-lysine 9 + succinate + formaldehyde + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9UPP1
-
-
Homo sapiens Q9Y4C1
-
-

Source Tissue

Source Tissue Comment Organism Textmining
HEK-293T cell
-
 Homo sapiens
-
LNCaP cell
-
 Homo sapiens
-
Phoenix-A cell
-
 Homo sapiens
-
prostate gland cancer cell
-
 Homo sapiens
-
RWPE-1 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the bifunctional enzyme catalyzes the demethylation of H3K9me2/me1 and H3K27me3/me2 (EC 1.14.11.68), as well as monomethylated histone H4 Lys20 residue (H4K20Me1). PHF8 acts upstream of KDM3A to regulate specific hypoxia-induced NED markers Homo sapiens ?
-
 ?
[histone H3]-N6,N6-dimethyl-L-lysine 9 + 2-oxoglutarate + O2
-
 Homo sapiens [histone H3]-N6-methyl-L-lysine 9 + succinate + formaldehyde + CO2
-
 ?
[histone H3]-N6-methyl-L-lysine 9 + 2-oxoglutarate + O2
-
 Homo sapiens [histone H3]-L-lysine 9 + succinate + formaldehyde + CO2
-
 ?
[histone H4]-N6-methyl-L-lysine 20 + 2-oxoglutarate + O2
-
 Homo sapiens [histone H4]-L-lysine 20 + succinate + formaldehyde + CO2
-
 ?

Synonyms

Synonyms Comment Organism
histone demethylase
-
 Homo sapiens
PHF8
-
 Homo sapiens

Expression

Organism Comment Expression
Homo sapiens treatment by hypoxia (1% O2, 6 days) but not by androgen deprivation or interleukin-6, leads to posttranscriptional upregulation of PHF8 in LNCaP cells up

General Information

General Information Comment Organism
malfunction PHF8 knockout inhibits the hypoxic activation of HIF1alpha protein, and attenuates the upregulation of KDM3A and ENO2 proteins. The fact that PHF8 knockdown by shRNAs in LNCaP cells does not affect HIF1A mRNA when compared with the impaired activation of HIF1alpha protein suggests that PHF8 indirectly regulates HIF1alpha protein Homo sapiens
physiological function histone demethylase PHF8 plays an essential role in hypoxia signaling. Knockdown or knockout of PHF8 reduces the activation of HIF1alpha and the induction of HIF1alpha target genes including KDM3A. PHF8 regulates hypoxia inducible genes mainly through sustaining the level of trimethylated histone 3 lysine 4 (H3K4me3). The positive role of PHF8 in hypoxia signaling extends to hypoxia-induced neuroendocrine differentiation, wherein PHF8 cooperates with KDM3A to regulate the expression of neuroendocrine differentiation genes. The role of PHF8 in hypoxia signaling is associated with the presence of full-length androgen receptor in castration-resistant prostate cancer cells Homo sapiens
physiological function histone demethylase PHF8 regulates hypoxia signaling through HIF1alpha and H3K4me3. Enzyme PHF8 binds and stabilizes histone H3 N6,N6,N6-trimethyl-L-lysine4, PHF8 is important in maintaining H3K4me3 levels on hypoxia-inducible genes, regulation, overview. PHF8 cooperates with KDM3A (EC 1.14.11.65) and PHF8 plays a role in hypoxia signaling. The regulation of hypoxia signaling by PHF8 is associated with androgen receptor AR status in prostate cancer cells Homo sapiens