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Literature summary for 1.14.11.60 extracted from

  • Siwinska, J.; Siatkowska, K.; Olry, A.; Grosjean, J.; Hehn, A.; Bourgaud, F.; Meharg, A.A.; Carey, M.; Lojkowska, E.; Ihnatowicz, A.
    Scopoletin 8-hydroxylase a novel enzyme involved in coumarin biosynthesis and iron-deficiency responses in Arabidopsis (2018), J. Exp. Bot., 69, 1735-1748 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.11.60

Cloned(Commentary)

Cloned (Comment) Organism
expression of S8H protein in Nicotiana benthamiana leaves Arabidopsis thaliana
heterologous expression in Escherichia coli Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
Fe2+ concentrations above 0.05 mM significantly reduce the enzyme activity, leading to decreased fraxetin synthesis Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.011
-
 scopoletin pH 8.0, 31.5°C Arabidopsis thaliana

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ enzyme efficiency is best in the presence of 0.05 mM Fe2+ Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
scopoletin + 2-oxoglutarate + O2 Arabidopsis thaliana the enzyme is involved in coumarin biosynthesis and iron-deficiency responses in Arabidopsis fraxetin + succinate + CO2
-
 ?
scopoletin + 2-oxoglutarate + O2 Arabidopsis thaliana Col-0 the enzyme is involved in coumarin biosynthesis and iron-deficiency responses in Arabidopsis fraxetin + succinate + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9LE86
-
-
Arabidopsis thaliana Col-0 Q9LE86
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
root
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
scopoletin + 2-oxoglutarate + O2
-
 Arabidopsis thaliana fraxetin + succinate + CO2
-
 ?
scopoletin + 2-oxoglutarate + O2 the enzyme is involved in coumarin biosynthesis and iron-deficiency responses in Arabidopsis Arabidopsis thaliana fraxetin + succinate + CO2
-
 ?
scopoletin + 2-oxoglutarate + O2
-
 Arabidopsis thaliana Col-0 fraxetin + succinate + CO2
-
 ?
scopoletin + 2-oxoglutarate + O2 the enzyme is involved in coumarin biosynthesis and iron-deficiency responses in Arabidopsis Arabidopsis thaliana Col-0 fraxetin + succinate + CO2
-
 ?

Synonyms

Synonyms Comment Organism
At3g12900 protein
-
 Arabidopsis thaliana
S8H
-
 Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
31.5
-
-
 Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.73
-
 scopoletin pH 8.0, 31.5°C Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Arabidopsis thaliana

General Information

General Information Comment Organism
metabolism the enzyme is involved in coumarin biosynthesis and is part of mechanisms used by plants to assimilate iron Arabidopsis thaliana

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
157.3
-
 scopoletin pH 8.0, 31.5°C Arabidopsis thaliana