Cloned (Comment) | Organism |
---|---|
expression of either the wild type JBP2 or the mutant JBP2 proteins in insect stage trypanosomes, which do not contain the modified base J and determined the base J content of each cell line, mutations made within the key catalytic domain ablates the ability of JBP2 to initiate de novo base J synthesis | Trypanosoma brucei |
Protein Variants | Comment | Organism |
---|---|---|
D393A | site-directed mutagenesis, mutation inside the catalytic domain, procyclic cells expressing mutant versions of JBP2 inside the catalytic domain | Trypanosoma brucei |
H391A | site-directed mutagenesis, mutation inside the catalytic domain, procyclic cells expressing mutant versions of JBP2, expression of the TH mutant H319A fails to stimulate base J formation | Trypanosoma brucei |
H441A | site-directed mutagenesis, mutation inside the catalytic domain, procyclic cells expressing mutant versions of JBP2 inside the catalytic domain | Trypanosoma brucei |
R445A | site-directed mutagenesis, mutation inside the catalytic domain, procyclic cells expressing mutant versions of JBP2 inside the catalytic domain | Trypanosoma brucei |
V459A | site-directed mutagenesis, control substitution of a residue outside the catalytic domain | Trypanosoma brucei |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
nucleus | - |
Trypanosoma brucei | 5634 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | 4 residues within this motif are critical for the coordination of Fe2+ and 2-oxoglutarate binding as well as functionality of this family of enzymes | Trypanosoma brucei | |
Fe2+ | acts as cofactor, 4 residues within this motif are critical for the coordination of Fe2+ and 2-oxoglutarate binding as well as functionality of this family of enzymes | Trypanosoma brucei |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
thymine + 2-oxoglutarate + O2 | Trypanosoma brucei | - |
5-hydroxymethyluracil + succinate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma brucei | - |
- |
- |
Trypanosoma brucei | Q57X81 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
29-13 cell | insect stage | Trypanosoma brucei | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
thymine + 2-oxoglutarate + O2 | - |
Trypanosoma brucei | 5-hydroxymethyluracil + succinate + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Fe2+/2-oxoglutarate dependent hydroxylase | - |
Trypanosoma brucei |
J binding protein 1 | - |
Trypanosoma brucei |
J binding protein 2 | - |
Trypanosoma brucei |
JBP1 | - |
Trypanosoma brucei |
JBP2 | - |
Trypanosoma brucei |
thymidine hydroxylase | - |
Trypanosoma brucei |