Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.11.55 extracted from

  • Hara, R.; Nishikawa, T.; Okuhara, T.; Koketsu, K.; Kino, K.
    Ectoine hydroxylase displays selective trans-3-hydroxylation activity towards L-proline (2019), Appl. Microbiol. Biotechnol., 103, 5689-5698 .
    View publication on PubMed
Search for more literature for 1.14.11.55

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Streptomyces cattleya

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline complete loss of activity Halomonas elongata
1,10-phenanthroline complete loss of activity Streptomyces cattleya
Co2+ more than 50% decrease in activity Halomonas elongata
Co2+ more than 50% decrease in activity Streptomyces cattleya
Cu2+ more than 50% decrease in activity Halomonas elongata
Cu2+ more than 50% decrease in activity Streptomyces cattleya
EDTA complete loss of activity Halomonas elongata
EDTA complete loss of activity Streptomyces cattleya
Mn2+ more than 50% decrease in activity Halomonas elongata
Mn2+ more than 50% decrease in activity Streptomyces cattleya
Ni2+ more than 50% decrease in activity Halomonas elongata
Ni2+ more than 50% decrease in activity Streptomyces cattleya
Zn2+ more than 50% decrease in activity Halomonas elongata
Zn2+ more than 50% decrease in activity Streptomyces cattleya

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.56
-
 ectoine pH 7.5, 30°C Halomonas elongata
1.7
-
 ectoine pH 7.5, 30°C Streptomyces cattleya
5.5
-
 L-proline pH 7.5, 30°C Halomonas elongata
17
-
 L-proline pH 7.5, 30°C Streptomyces cattleya

Organism

Organism UniProt Comment Textmining
Halomonas elongata E1VA04
-
-
Halomonas elongata DSM 2581 E1VA04
-
-
Streptomyces cattleya F8JYW1
-
-
Streptomyces cattleya NBRC 14057 F8JYW1
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ectoine + 2-oxoglutarate + O2
-
 Halomonas elongata 5-hydroxyectoine + succinate + CO2
-
 ?
ectoine + 2-oxoglutarate + O2
-
 Streptomyces cattleya 5-hydroxyectoine + succinate + CO2
-
 ?
ectoine + 2-oxoglutarate + O2
-
 Halomonas elongata DSM 2581 5-hydroxyectoine + succinate + CO2
-
 ?
ectoine + 2-oxoglutarate + O2
-
 Streptomyces cattleya NBRC 14057 5-hydroxyectoine + succinate + CO2
-
 ?
L-proline + 2-oxoglutarate + O2
-
 Halomonas elongata trans-3-hydroxyproline + succinate + CO2
-
 ?
L-proline + 2-oxoglutarate + O2
-
 Streptomyces cattleya trans-3-hydroxyproline + succinate + CO2
-
 ?
L-proline + 2-oxoglutarate + O2
-
 Halomonas elongata DSM 2581 trans-3-hydroxyproline + succinate + CO2
-
 ?
L-proline + 2-oxoglutarate + O2
-
 Streptomyces cattleya NBRC 14057 trans-3-hydroxyproline + succinate + CO2
-
 ?

Synonyms

Synonyms Comment Organism
ectD
-
 Halomonas elongata
ectD
-
 Streptomyces cattleya
HELO_4008
-
 Halomonas elongata
SCATT_10620
-
 Streptomyces cattleya

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Halomonas elongata
30
-
-
 Streptomyces cattleya

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.029
-
 L-proline pH 7.5, 30°C Halomonas elongata
0.11
-
 L-proline pH 7.5, 30°C Streptomyces cattleya
0.22
-
 ectoine pH 7.5, 30°C Halomonas elongata
0.55
-
 ectoine pH 7.5, 30°C Streptomyces cattleya

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Halomonas elongata
7.5
-
-
 Streptomyces cattleya

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0007
-
 L-proline pH 7.5, 30°C Streptomyces cattleya
0.0053
-
 L-proline pH 7.5, 30°C Halomonas elongata
0.33
-
 ectoine pH 7.5, 30°C Streptomyces cattleya
0.39
-
 ectoine pH 7.5, 30°C Halomonas elongata