BRENDA - Enzyme Database
show all sequences of 1.14.11.55

Biochemical properties of ectoine hydroxylases from extremophiles and their wider taxonomic distribution among microorganisms

Widderich, N.; Hoeppner, A.; Pittelkow, M.; Heider, J.; Smits, S.H.; Bremer, E.; PLoS ONE 9, e93809 (2014)

Data extracted from this reference:

Crystallization (Commentary)
Crystallization (Commentary)
Organism
to 1.9 A resolution, and comparison of iron-bound and apo structure. The iron ligand is bound via interaction with histidine side-chains His146 and His248, and the side-chain of Asp-148. These residues form a conserved H6D/E…H motif, the so-called 2-His-1-carboxylate facial triad
Virgibacillus salexigens
Inhibitors
Inhibitors
Commentary
Organism
Structure
NaCl
maximum activity in presence of 50 mM KCl. High concentrations of NaCl are inhibitory
Acidiphilium cryptum
NaCl
maximum activity in presence of 150 mM KCl. High concentrations of NaCl are inhibitory
Alkalilimnicola ehrlichii
NaCl
maximum activity in presence of 100 mM KCl. High concentrations of NaCl are inhibitory
Halomonas elongata
NaCl
maximum activity in presence of 150 mM KCl. High concentrations of NaCl are inhibitory
Paenibacillus lautus
NaCl
maximum activity in presence of 100 mM KCl. High concentrations of NaCl are inhibitory
Pseudomonas stutzeri
NaCl
maximum activity in presence of 100 mM KCl. High concentrations of NaCl are inhibitory
Sphingopyxis alaskensis
NaCl
maximum activity in presence of 100 mM KCl. High concentrations of NaCl are inhibitory
Virgibacillus salexigens
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7
-
2-oxoglutarate
pH 7.5, 32°C
Sphingopyxis alaskensis
3.9
-
2-oxoglutarate
pH 7.5, 32°C
Paenibacillus lautus
4.1
-
2-oxoglutarate
pH 7.5, 32°C
Acidiphilium cryptum
4.6
-
2-oxoglutarate
pH 7.5, 32°C
Pseudomonas stutzeri
4.8
-
2-oxoglutarate
pH 7.5, 32°C
Halomonas elongata
4.9
-
2-oxoglutarate
pH 7.5, 32°C
Virgibacillus salexigens
5
-
2-oxoglutarate
pH 7.5, 32°C
Alkalilimnicola ehrlichii
5.7
-
ectoine
pH 7.5, 32°C
Halomonas elongata
5.9
-
ectoine
pH 7.5, 32°C
Virgibacillus salexigens
6.2
-
ectoine
pH 7.5, 32°C
Pseudomonas stutzeri
9
-
ectoine
pH 7.5, 32°C
Alkalilimnicola ehrlichii
9.5
-
ectoine
pH 7.5, 32°C
Paenibacillus lautus
9.8
-
ectoine
pH 7.5, 32°C
Sphingopyxis alaskensis
10
-
ectoine
pH 7.5, 32°C
Acidiphilium cryptum
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Iron
the iron ligand is bound via interaction with histidine side-chains His146 and His248, and the side-chain of Asp-148. These residues form a conserved H6D/E…H motif, the so-called 2-His-1-carboxylate facial triad
Acidiphilium cryptum
Iron
the iron ligand is bound via interaction with histidine side-chains His146 and His248, and the side-chain of Asp-148. These residues form a conserved H6D/E…H motif, the so-called 2-His-1-carboxylate facial triad
Alkalilimnicola ehrlichii
Iron
the iron ligand is bound via interaction with histidine side-chains His146 and His248, and the side-chain of Asp-148. These residues form a conserved H6D/E…H motif, the so-called 2-His-1-carboxylate facial triad
Halomonas elongata
Iron
the iron ligand is bound via interaction with histidine side-chains His146 and His248, and the side-chain of Asp-148. These residues form a conserved H6D/E…H motif, the so-called 2-His-1-carboxylate facial triad
Paenibacillus lautus
Iron
the iron ligand is bound via interaction with histidine side-chains His146 and His248, and the side-chain of Asp-148. These residues form a conserved H6D/E…H motif, the so-called 2-His-1-carboxylate facial triad
Pseudomonas stutzeri
Iron
the iron ligand is bound via interaction with histidine side-chains His146 and His248, and the side-chain of Asp-148. These residues form a conserved H6D/E…H motif, the so-called 2-His-1-carboxylate facial triad
Sphingopyxis alaskensis
Iron
the iron ligand is bound via interaction with histidine side-chains His146 and His248, and the side-chain of Asp-148. These residues form a conserved H6D/E…H motif, the so-called 2-His-1-carboxylate facial triad
Virgibacillus salexigens
KCl
maximum activity in presence of 100 mM KCl
Acidiphilium cryptum
KCl
maximum activity in presence of 150 mM KCl
Alkalilimnicola ehrlichii
KCl
maximum activity in presence of 150 mM KCl
Halomonas elongata
KCl
maximum activity in presence of 200 mM KCl
Paenibacillus lautus
KCl
maximum activity in presence of 150 mM KCl
Pseudomonas stutzeri
KCl
maximum activity in presence of 100 mM KCl
Sphingopyxis alaskensis
KCl
maximum activity in presence of 150 mM KCl
Virgibacillus salexigens
NaCl
maximum activity in presence of 50 mM KCl. High concentrations of NaCl are inhibitory
Acidiphilium cryptum
NaCl
maximum activity in presence of 150 mM KCl. High concentrations of NaCl are inhibitory
Alkalilimnicola ehrlichii
NaCl
maximum activity in presence of 100 mM KCl. High concentrations of NaCl are inhibitory
Halomonas elongata
NaCl
maximum activity in presence of 150 mM KCl. High concentrations of NaCl are inhibitory
Paenibacillus lautus
NaCl
maximum activity in presence of 100 mM KCl. High concentrations of NaCl are inhibitory
Pseudomonas stutzeri
NaCl
maximum activity in presence of 100 mM KCl. High concentrations of NaCl are inhibitory
Sphingopyxis alaskensis
NaCl
maximum activity in presence of 100 mM KCl. High concentrations of NaCl are inhibitory
Virgibacillus salexigens
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
34100
-
-
Acidiphilium cryptum
34100
-
-
Sphingopyxis alaskensis
34200
-
x * 34200, SDS-PAGE
Pseudomonas stutzeri
34300
-
x * 34300, SDS-PAGE
Alkalilimnicola ehrlichii
34400
-
x * 34400, SDS-PAGE
Virgibacillus salexigens
34800
-
x * 34800, SDS-PAGE
Paenibacillus lautus
37400
-
-
Halomonas elongata
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
ectoine + 2-oxoglutarate + O2
Pseudomonas stutzeri
-
5-hydroxyectoine + succinate + CO2
-
-
ir
ectoine + 2-oxoglutarate + O2
Virgibacillus salexigens
-
5-hydroxyectoine + succinate + CO2
-
-
ir
ectoine + 2-oxoglutarate + O2
Sphingopyxis alaskensis
-
5-hydroxyectoine + succinate + CO2
-
-
ir
ectoine + 2-oxoglutarate + O2
Halomonas elongata
-
5-hydroxyectoine + succinate + CO2
-
-
ir
ectoine + 2-oxoglutarate + O2
Paenibacillus lautus
-
5-hydroxyectoine + succinate + CO2
-
-
ir
ectoine + 2-oxoglutarate + O2
Alkalilimnicola ehrlichii
-
5-hydroxyectoine + succinate + CO2
-
-
ir
ectoine + 2-oxoglutarate + O2
Acidiphilium cryptum
-
5-hydroxyectoine + succinate + CO2
-
-
ir
ectoine + 2-oxoglutarate + O2
Pseudomonas stutzeri A1501
-
5-hydroxyectoine + succinate + CO2
-
-
ir
ectoine + 2-oxoglutarate + O2
Sphingopyxis alaskensis DSM 13593
-
5-hydroxyectoine + succinate + CO2
-
-
ir
ectoine + 2-oxoglutarate + O2
Halomonas elongata DSM 2581
-
5-hydroxyectoine + succinate + CO2
-
-
ir
additional information
Pseudomonas stutzeri
ectoine hydroxylase operates exclusively in one direction under physiologically relevant conditions to direct the formation of 5-hydroxyectoine from the precursor ectoine
?
-
-
-
additional information
Virgibacillus salexigens
ectoine hydroxylase operates exclusively in one direction under physiologically relevant conditions to direct the formation of 5-hydroxyectoine from the precursor ectoine
?
-
-
-
additional information
Sphingopyxis alaskensis
ectoine hydroxylase operates exclusively in one direction under physiologically relevant conditions to direct the formation of 5-hydroxyectoine from the precursor ectoine
?
-
-
-
additional information
Halomonas elongata
ectoine hydroxylase operates exclusively in one direction under physiologically relevant conditions to direct the formation of 5-hydroxyectoine from the precursor ectoine
?
-
-
-
additional information
Paenibacillus lautus
ectoine hydroxylase operates exclusively in one direction under physiologically relevant conditions to direct the formation of 5-hydroxyectoine from the precursor ectoine
?
-
-
-
additional information
Alkalilimnicola ehrlichii
ectoine hydroxylase operates exclusively in one direction under physiologically relevant conditions to direct the formation of 5-hydroxyectoine from the precursor ectoine
?
-
-
-
additional information
Acidiphilium cryptum
ectoine hydroxylase operates exclusively in one direction under physiologically relevant conditions to direct the formation of 5-hydroxyectoine from the precursor ectoine
?
-
-
-
additional information
Pseudomonas stutzeri A1501
ectoine hydroxylase operates exclusively in one direction under physiologically relevant conditions to direct the formation of 5-hydroxyectoine from the precursor ectoine
?
-
-
-
additional information
Sphingopyxis alaskensis DSM 13593
ectoine hydroxylase operates exclusively in one direction under physiologically relevant conditions to direct the formation of 5-hydroxyectoine from the precursor ectoine
?
-
-
-
additional information
Halomonas elongata DSM 2581
ectoine hydroxylase operates exclusively in one direction under physiologically relevant conditions to direct the formation of 5-hydroxyectoine from the precursor ectoine
?
-
-
-
Organism
Organism
UniProt
Commentary
Textmining
Acidiphilium cryptum
JN019030
ectD gene optimized for the expression in Escherichia coli
-
Alkalilimnicola ehrlichii
JN019031
ectD gene optimized for the expression in Escherichia coli
-
Halomonas elongata
E1VA04
-
-
Halomonas elongata DSM 2581
E1VA04
-
-
Paenibacillus lautus
A0A1R1AUM9
ectD gene optimized for the expression in Escherichia coli
-
Pseudomonas stutzeri
-
-
-
Pseudomonas stutzeri A1501
-
-
-
Sphingopyxis alaskensis
Q1GNW5
-
-
Sphingopyxis alaskensis DSM 13593
Q1GNW5
-
-
Virgibacillus salexigens
Q2TDY4
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
ectoine + 2-oxoglutarate + O2
-
741304
Pseudomonas stutzeri
5-hydroxyectoine + succinate + CO2
-
-
-
ir
ectoine + 2-oxoglutarate + O2
-
741304
Virgibacillus salexigens
5-hydroxyectoine + succinate + CO2
-
-
-
ir
ectoine + 2-oxoglutarate + O2
-
741304
Sphingopyxis alaskensis
5-hydroxyectoine + succinate + CO2
-
-
-
ir
ectoine + 2-oxoglutarate + O2
-
741304
Halomonas elongata
5-hydroxyectoine + succinate + CO2
-
-
-
ir
ectoine + 2-oxoglutarate + O2
-
741304
Paenibacillus lautus
5-hydroxyectoine + succinate + CO2
-
-
-
ir
ectoine + 2-oxoglutarate + O2
-
741304
Alkalilimnicola ehrlichii
5-hydroxyectoine + succinate + CO2
-
-
-
ir
ectoine + 2-oxoglutarate + O2
-
741304
Acidiphilium cryptum
5-hydroxyectoine + succinate + CO2
-
-
-
ir
ectoine + 2-oxoglutarate + O2
-
741304
Pseudomonas stutzeri A1501
5-hydroxyectoine + succinate + CO2
-
-
-
ir
ectoine + 2-oxoglutarate + O2
-
741304
Sphingopyxis alaskensis DSM 13593
5-hydroxyectoine + succinate + CO2
-
-
-
ir
ectoine + 2-oxoglutarate + O2
-
741304
Halomonas elongata DSM 2581
5-hydroxyectoine + succinate + CO2
-
-
-
ir
additional information
ectoine hydroxylase operates exclusively in one direction under physiologically relevant conditions to direct the formation of 5-hydroxyectoine from the precursor ectoine
741304
Pseudomonas stutzeri
?
-
-
-
-
additional information
ectoine hydroxylase operates exclusively in one direction under physiologically relevant conditions to direct the formation of 5-hydroxyectoine from the precursor ectoine
741304
Virgibacillus salexigens
?
-
-
-
-
additional information
ectoine hydroxylase operates exclusively in one direction under physiologically relevant conditions to direct the formation of 5-hydroxyectoine from the precursor ectoine
741304
Sphingopyxis alaskensis
?
-
-
-
-
additional information
ectoine hydroxylase operates exclusively in one direction under physiologically relevant conditions to direct the formation of 5-hydroxyectoine from the precursor ectoine
741304
Halomonas elongata
?
-
-
-
-
additional information
ectoine hydroxylase operates exclusively in one direction under physiologically relevant conditions to direct the formation of 5-hydroxyectoine from the precursor ectoine
741304
Paenibacillus lautus
?
-
-
-
-
additional information
ectoine hydroxylase operates exclusively in one direction under physiologically relevant conditions to direct the formation of 5-hydroxyectoine from the precursor ectoine
741304
Alkalilimnicola ehrlichii
?
-
-
-
-
additional information
ectoine hydroxylase operates exclusively in one direction under physiologically relevant conditions to direct the formation of 5-hydroxyectoine from the precursor ectoine
741304
Acidiphilium cryptum
?
-
-
-
-
additional information
ectoine hydroxylase operates exclusively in one direction under physiologically relevant conditions to direct the formation of 5-hydroxyectoine from the precursor ectoine
741304
Pseudomonas stutzeri A1501
?
-
-
-
-
additional information
ectoine hydroxylase operates exclusively in one direction under physiologically relevant conditions to direct the formation of 5-hydroxyectoine from the precursor ectoine
741304
Sphingopyxis alaskensis DSM 13593
?
-
-
-
-
additional information
ectoine hydroxylase operates exclusively in one direction under physiologically relevant conditions to direct the formation of 5-hydroxyectoine from the precursor ectoine
741304
Halomonas elongata DSM 2581
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
?
x * 34100, SDS-PAGE
Acidiphilium cryptum
?
x * 34300, SDS-PAGE
Alkalilimnicola ehrlichii
?
x * 37400, SDS-PAGE
Halomonas elongata
?
x * 34800, SDS-PAGE
Paenibacillus lautus
?
x * 34200, SDS-PAGE
Pseudomonas stutzeri
?
x * 34100, SDS-PAGE
Sphingopyxis alaskensis
?
x * 34400, SDS-PAGE
Virgibacillus salexigens
Synonyms
Synonyms
Commentary
Organism
ectD
-
Acidiphilium cryptum
ectD
-
Alkalilimnicola ehrlichii
ectD
-
Halomonas elongata
ectD
-
Paenibacillus lautus
ectD
-
Pseudomonas stutzeri
ectD
-
Sphingopyxis alaskensis
ectD
-
Virgibacillus salexigens
Sala_2952
-
Sphingopyxis alaskensis
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
32
-
-
Acidiphilium cryptum
32
-
-
Halomonas elongata
32
-
-
Virgibacillus salexigens
35
-
-
Alkalilimnicola ehrlichii
35
-
-
Pseudomonas stutzeri
40
-
-
Paenibacillus lautus
40
-
-
Sphingopyxis alaskensis
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
5
47
-
Halomonas elongata
5
50
-
Sphingopyxis alaskensis
5
50
-
Virgibacillus salexigens
10
47
-
Acidiphilium cryptum
10
50
-
Pseudomonas stutzeri
15
45
-
Alkalilimnicola ehrlichii
15
50
-
Paenibacillus lautus
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.2
-
ectoine
pH 7.5, 32°C
Alkalilimnicola ehrlichii
1.2
-
ectoine
pH 7.5, 32°C
Sphingopyxis alaskensis
1.6
-
ectoine
pH 7.5, 32°C
Paenibacillus lautus
2.8
-
ectoine
pH 7.5, 32°C
Halomonas elongata
3.4
-
ectoine
pH 7.5, 32°C
Acidiphilium cryptum
7.7
-
ectoine
pH 7.5, 32°C
Virgibacillus salexigens
8.9
-
ectoine
pH 7.5, 32°C
Pseudomonas stutzeri
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Alkalilimnicola ehrlichii
7.5
-
-
Paenibacillus lautus
7.5
-
-
Pseudomonas stutzeri
7.5
-
-
Virgibacillus salexigens
8
-
-
Acidiphilium cryptum
8
-
-
Halomonas elongata
8
-
-
Sphingopyxis alaskensis
pH Range
pH Minimum
pH Maximum
Commentary
Organism
5.5
9.6
-
Acidiphilium cryptum
5.5
9.6
-
Paenibacillus lautus
5.5
9.6
-
Pseudomonas stutzeri
5.5
9.6
-
Sphingopyxis alaskensis
5.5
9.6
-
Virgibacillus salexigens
6.5
9.6
-
Alkalilimnicola ehrlichii
6.5
9.6
-
Halomonas elongata
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Pseudomonas stutzeri
calculated
-
5.5
Sphingopyxis alaskensis
calculated
-
5.5
Paenibacillus lautus
calculated
-
5.6
Alkalilimnicola ehrlichii
calculated
-
5.7
Acidiphilium cryptum
calculated
-
5.8
Halomonas elongata
calculated
-
5.8
Virgibacillus salexigens
calculated
-
5.8
Crystallization (Commentary) (protein specific)
Crystallization
Organism
to 1.9 A resolution, and comparison of iron-bound and apo structure. The iron ligand is bound via interaction with histidine side-chains His146 and His248, and the side-chain of Asp-148. These residues form a conserved H6D/E…H motif, the so-called 2-His-1-carboxylate facial triad
Virgibacillus salexigens
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
NaCl
maximum activity in presence of 50 mM KCl. High concentrations of NaCl are inhibitory
Acidiphilium cryptum
NaCl
maximum activity in presence of 150 mM KCl. High concentrations of NaCl are inhibitory
Alkalilimnicola ehrlichii
NaCl
maximum activity in presence of 100 mM KCl. High concentrations of NaCl are inhibitory
Halomonas elongata
NaCl
maximum activity in presence of 150 mM KCl. High concentrations of NaCl are inhibitory
Paenibacillus lautus
NaCl
maximum activity in presence of 100 mM KCl. High concentrations of NaCl are inhibitory
Pseudomonas stutzeri
NaCl
maximum activity in presence of 100 mM KCl. High concentrations of NaCl are inhibitory
Sphingopyxis alaskensis
NaCl
maximum activity in presence of 100 mM KCl. High concentrations of NaCl are inhibitory
Virgibacillus salexigens
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7
-
2-oxoglutarate
pH 7.5, 32°C
Sphingopyxis alaskensis
3.9
-
2-oxoglutarate
pH 7.5, 32°C
Paenibacillus lautus
4.1
-
2-oxoglutarate
pH 7.5, 32°C
Acidiphilium cryptum
4.6
-
2-oxoglutarate
pH 7.5, 32°C
Pseudomonas stutzeri
4.8
-
2-oxoglutarate
pH 7.5, 32°C
Halomonas elongata
4.9
-
2-oxoglutarate
pH 7.5, 32°C
Virgibacillus salexigens
5
-
2-oxoglutarate
pH 7.5, 32°C
Alkalilimnicola ehrlichii
5.7
-
ectoine
pH 7.5, 32°C
Halomonas elongata
5.9
-
ectoine
pH 7.5, 32°C
Virgibacillus salexigens
6.2
-
ectoine
pH 7.5, 32°C
Pseudomonas stutzeri
9
-
ectoine
pH 7.5, 32°C
Alkalilimnicola ehrlichii
9.5
-
ectoine
pH 7.5, 32°C
Paenibacillus lautus
9.8
-
ectoine
pH 7.5, 32°C
Sphingopyxis alaskensis
10
-
ectoine
pH 7.5, 32°C
Acidiphilium cryptum
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Iron
the iron ligand is bound via interaction with histidine side-chains His146 and His248, and the side-chain of Asp-148. These residues form a conserved H6D/E…H motif, the so-called 2-His-1-carboxylate facial triad
Acidiphilium cryptum
Iron
the iron ligand is bound via interaction with histidine side-chains His146 and His248, and the side-chain of Asp-148. These residues form a conserved H6D/E…H motif, the so-called 2-His-1-carboxylate facial triad
Alkalilimnicola ehrlichii
Iron
the iron ligand is bound via interaction with histidine side-chains His146 and His248, and the side-chain of Asp-148. These residues form a conserved H6D/E…H motif, the so-called 2-His-1-carboxylate facial triad
Halomonas elongata
Iron
the iron ligand is bound via interaction with histidine side-chains His146 and His248, and the side-chain of Asp-148. These residues form a conserved H6D/E…H motif, the so-called 2-His-1-carboxylate facial triad
Paenibacillus lautus
Iron
the iron ligand is bound via interaction with histidine side-chains His146 and His248, and the side-chain of Asp-148. These residues form a conserved H6D/E…H motif, the so-called 2-His-1-carboxylate facial triad
Pseudomonas stutzeri
Iron
the iron ligand is bound via interaction with histidine side-chains His146 and His248, and the side-chain of Asp-148. These residues form a conserved H6D/E…H motif, the so-called 2-His-1-carboxylate facial triad
Sphingopyxis alaskensis
Iron
the iron ligand is bound via interaction with histidine side-chains His146 and His248, and the side-chain of Asp-148. These residues form a conserved H6D/E…H motif, the so-called 2-His-1-carboxylate facial triad
Virgibacillus salexigens
KCl
maximum activity in presence of 100 mM KCl
Acidiphilium cryptum
KCl
maximum activity in presence of 150 mM KCl
Alkalilimnicola ehrlichii
KCl
maximum activity in presence of 150 mM KCl
Halomonas elongata
KCl
maximum activity in presence of 200 mM KCl
Paenibacillus lautus
KCl
maximum activity in presence of 150 mM KCl
Pseudomonas stutzeri
KCl
maximum activity in presence of 100 mM KCl
Sphingopyxis alaskensis
KCl
maximum activity in presence of 150 mM KCl
Virgibacillus salexigens
NaCl
maximum activity in presence of 50 mM KCl. High concentrations of NaCl are inhibitory
Acidiphilium cryptum
NaCl
maximum activity in presence of 150 mM KCl. High concentrations of NaCl are inhibitory
Alkalilimnicola ehrlichii
NaCl
maximum activity in presence of 100 mM KCl. High concentrations of NaCl are inhibitory
Halomonas elongata
NaCl
maximum activity in presence of 150 mM KCl. High concentrations of NaCl are inhibitory
Paenibacillus lautus
NaCl
maximum activity in presence of 100 mM KCl. High concentrations of NaCl are inhibitory
Pseudomonas stutzeri
NaCl
maximum activity in presence of 100 mM KCl. High concentrations of NaCl are inhibitory
Sphingopyxis alaskensis
NaCl
maximum activity in presence of 100 mM KCl. High concentrations of NaCl are inhibitory
Virgibacillus salexigens
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
34100
-
-
Acidiphilium cryptum
34100
-
-
Sphingopyxis alaskensis
34200
-
x * 34200, SDS-PAGE
Pseudomonas stutzeri
34300
-
x * 34300, SDS-PAGE
Alkalilimnicola ehrlichii
34400
-
x * 34400, SDS-PAGE
Virgibacillus salexigens
34800
-
x * 34800, SDS-PAGE
Paenibacillus lautus
37400
-
-
Halomonas elongata
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
ectoine + 2-oxoglutarate + O2
Pseudomonas stutzeri
-
5-hydroxyectoine + succinate + CO2
-
-
ir
ectoine + 2-oxoglutarate + O2
Virgibacillus salexigens
-
5-hydroxyectoine + succinate + CO2
-
-
ir
ectoine + 2-oxoglutarate + O2
Sphingopyxis alaskensis
-
5-hydroxyectoine + succinate + CO2
-
-
ir
ectoine + 2-oxoglutarate + O2
Halomonas elongata
-
5-hydroxyectoine + succinate + CO2
-
-
ir
ectoine + 2-oxoglutarate + O2
Paenibacillus lautus
-
5-hydroxyectoine + succinate + CO2
-
-
ir
ectoine + 2-oxoglutarate + O2
Alkalilimnicola ehrlichii
-
5-hydroxyectoine + succinate + CO2
-
-
ir
ectoine + 2-oxoglutarate + O2
Acidiphilium cryptum
-
5-hydroxyectoine + succinate + CO2
-
-
ir
ectoine + 2-oxoglutarate + O2
Pseudomonas stutzeri A1501
-
5-hydroxyectoine + succinate + CO2
-
-
ir
ectoine + 2-oxoglutarate + O2
Sphingopyxis alaskensis DSM 13593
-
5-hydroxyectoine + succinate + CO2
-
-
ir
ectoine + 2-oxoglutarate + O2
Halomonas elongata DSM 2581
-
5-hydroxyectoine + succinate + CO2
-
-
ir
additional information
Pseudomonas stutzeri
ectoine hydroxylase operates exclusively in one direction under physiologically relevant conditions to direct the formation of 5-hydroxyectoine from the precursor ectoine
?
-
-
-
additional information
Virgibacillus salexigens
ectoine hydroxylase operates exclusively in one direction under physiologically relevant conditions to direct the formation of 5-hydroxyectoine from the precursor ectoine
?
-
-
-
additional information
Sphingopyxis alaskensis
ectoine hydroxylase operates exclusively in one direction under physiologically relevant conditions to direct the formation of 5-hydroxyectoine from the precursor ectoine
?
-
-
-
additional information
Halomonas elongata
ectoine hydroxylase operates exclusively in one direction under physiologically relevant conditions to direct the formation of 5-hydroxyectoine from the precursor ectoine
?
-
-
-
additional information
Paenibacillus lautus
ectoine hydroxylase operates exclusively in one direction under physiologically relevant conditions to direct the formation of 5-hydroxyectoine from the precursor ectoine
?
-
-
-
additional information
Alkalilimnicola ehrlichii
ectoine hydroxylase operates exclusively in one direction under physiologically relevant conditions to direct the formation of 5-hydroxyectoine from the precursor ectoine
?
-
-
-
additional information
Acidiphilium cryptum
ectoine hydroxylase operates exclusively in one direction under physiologically relevant conditions to direct the formation of 5-hydroxyectoine from the precursor ectoine
?
-
-
-
additional information
Pseudomonas stutzeri A1501
ectoine hydroxylase operates exclusively in one direction under physiologically relevant conditions to direct the formation of 5-hydroxyectoine from the precursor ectoine
?
-
-
-
additional information
Sphingopyxis alaskensis DSM 13593
ectoine hydroxylase operates exclusively in one direction under physiologically relevant conditions to direct the formation of 5-hydroxyectoine from the precursor ectoine
?
-
-
-
additional information
Halomonas elongata DSM 2581
ectoine hydroxylase operates exclusively in one direction under physiologically relevant conditions to direct the formation of 5-hydroxyectoine from the precursor ectoine
?
-
-
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
ectoine + 2-oxoglutarate + O2
-
741304
Pseudomonas stutzeri
5-hydroxyectoine + succinate + CO2
-
-
-
ir
ectoine + 2-oxoglutarate + O2
-
741304
Virgibacillus salexigens
5-hydroxyectoine + succinate + CO2
-
-
-
ir
ectoine + 2-oxoglutarate + O2
-
741304
Sphingopyxis alaskensis
5-hydroxyectoine + succinate + CO2
-
-
-
ir
ectoine + 2-oxoglutarate + O2
-
741304
Halomonas elongata
5-hydroxyectoine + succinate + CO2
-
-
-
ir
ectoine + 2-oxoglutarate + O2
-
741304
Paenibacillus lautus
5-hydroxyectoine + succinate + CO2
-
-
-
ir
ectoine + 2-oxoglutarate + O2
-
741304
Alkalilimnicola ehrlichii
5-hydroxyectoine + succinate + CO2
-
-
-
ir
ectoine + 2-oxoglutarate + O2
-
741304
Acidiphilium cryptum
5-hydroxyectoine + succinate + CO2
-
-
-
ir
ectoine + 2-oxoglutarate + O2
-
741304
Pseudomonas stutzeri A1501
5-hydroxyectoine + succinate + CO2
-
-
-
ir
ectoine + 2-oxoglutarate + O2
-
741304
Sphingopyxis alaskensis DSM 13593
5-hydroxyectoine + succinate + CO2
-
-
-
ir
ectoine + 2-oxoglutarate + O2
-
741304
Halomonas elongata DSM 2581
5-hydroxyectoine + succinate + CO2
-
-
-
ir
additional information
ectoine hydroxylase operates exclusively in one direction under physiologically relevant conditions to direct the formation of 5-hydroxyectoine from the precursor ectoine
741304
Pseudomonas stutzeri
?
-
-
-
-
additional information
ectoine hydroxylase operates exclusively in one direction under physiologically relevant conditions to direct the formation of 5-hydroxyectoine from the precursor ectoine
741304
Virgibacillus salexigens
?
-
-
-
-
additional information
ectoine hydroxylase operates exclusively in one direction under physiologically relevant conditions to direct the formation of 5-hydroxyectoine from the precursor ectoine
741304
Sphingopyxis alaskensis
?
-
-
-
-
additional information
ectoine hydroxylase operates exclusively in one direction under physiologically relevant conditions to direct the formation of 5-hydroxyectoine from the precursor ectoine
741304
Halomonas elongata
?
-
-
-
-
additional information
ectoine hydroxylase operates exclusively in one direction under physiologically relevant conditions to direct the formation of 5-hydroxyectoine from the precursor ectoine
741304
Paenibacillus lautus
?
-
-
-
-
additional information
ectoine hydroxylase operates exclusively in one direction under physiologically relevant conditions to direct the formation of 5-hydroxyectoine from the precursor ectoine
741304
Alkalilimnicola ehrlichii
?
-
-
-
-
additional information
ectoine hydroxylase operates exclusively in one direction under physiologically relevant conditions to direct the formation of 5-hydroxyectoine from the precursor ectoine
741304
Acidiphilium cryptum
?
-
-
-
-
additional information
ectoine hydroxylase operates exclusively in one direction under physiologically relevant conditions to direct the formation of 5-hydroxyectoine from the precursor ectoine
741304
Pseudomonas stutzeri A1501
?
-
-
-
-
additional information
ectoine hydroxylase operates exclusively in one direction under physiologically relevant conditions to direct the formation of 5-hydroxyectoine from the precursor ectoine
741304
Sphingopyxis alaskensis DSM 13593
?
-
-
-
-
additional information
ectoine hydroxylase operates exclusively in one direction under physiologically relevant conditions to direct the formation of 5-hydroxyectoine from the precursor ectoine
741304
Halomonas elongata DSM 2581
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 34100, SDS-PAGE
Acidiphilium cryptum
?
x * 34300, SDS-PAGE
Alkalilimnicola ehrlichii
?
x * 37400, SDS-PAGE
Halomonas elongata
?
x * 34800, SDS-PAGE
Paenibacillus lautus
?
x * 34200, SDS-PAGE
Pseudomonas stutzeri
?
x * 34100, SDS-PAGE
Sphingopyxis alaskensis
?
x * 34400, SDS-PAGE
Virgibacillus salexigens
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
32
-
-
Acidiphilium cryptum
32
-
-
Halomonas elongata
32
-
-
Virgibacillus salexigens
35
-
-
Alkalilimnicola ehrlichii
35
-
-
Pseudomonas stutzeri
40
-
-
Paenibacillus lautus
40
-
-
Sphingopyxis alaskensis
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
5
47
-
Halomonas elongata
5
50
-
Sphingopyxis alaskensis
5
50
-
Virgibacillus salexigens
10
47
-
Acidiphilium cryptum
10
50
-
Pseudomonas stutzeri
15
45
-
Alkalilimnicola ehrlichii
15
50
-
Paenibacillus lautus
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.2
-
ectoine
pH 7.5, 32°C
Alkalilimnicola ehrlichii
1.2
-
ectoine
pH 7.5, 32°C
Sphingopyxis alaskensis
1.6
-
ectoine
pH 7.5, 32°C
Paenibacillus lautus
2.8
-
ectoine
pH 7.5, 32°C
Halomonas elongata
3.4
-
ectoine
pH 7.5, 32°C
Acidiphilium cryptum
7.7
-
ectoine
pH 7.5, 32°C
Virgibacillus salexigens
8.9
-
ectoine
pH 7.5, 32°C
Pseudomonas stutzeri
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Alkalilimnicola ehrlichii
7.5
-
-
Paenibacillus lautus
7.5
-
-
Pseudomonas stutzeri
7.5
-
-
Virgibacillus salexigens
8
-
-
Acidiphilium cryptum
8
-
-
Halomonas elongata
8
-
-
Sphingopyxis alaskensis
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
5.5
9.6
-
Acidiphilium cryptum
5.5
9.6
-
Paenibacillus lautus
5.5
9.6
-
Pseudomonas stutzeri
5.5
9.6
-
Sphingopyxis alaskensis
5.5
9.6
-
Virgibacillus salexigens
6.5
9.6
-
Alkalilimnicola ehrlichii
6.5
9.6
-
Halomonas elongata
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Pseudomonas stutzeri
calculated
-
5.5
Sphingopyxis alaskensis
calculated
-
5.5
Paenibacillus lautus
calculated
-
5.6
Alkalilimnicola ehrlichii
calculated
-
5.7
Acidiphilium cryptum
calculated
-
5.8
Halomonas elongata
calculated
-
5.8
Virgibacillus salexigens
calculated
-
5.8
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.12
-
ectoine
pH 7.5, 32°C
Sphingopyxis alaskensis
0.13
-
ectoine
pH 7.5, 32°C
Alkalilimnicola ehrlichii
0.17
-
ectoine
pH 7.5, 32°C
Paenibacillus lautus
0.34
-
ectoine
pH 7.5, 32°C
Acidiphilium cryptum
0.49
-
ectoine
pH 7.5, 32°C
Halomonas elongata
1.31
-
ectoine
pH 7.5, 32°C
Virgibacillus salexigens
1.44
-
ectoine
pH 7.5, 32°C
Pseudomonas stutzeri
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.12
-
ectoine
pH 7.5, 32°C
Sphingopyxis alaskensis
0.13
-
ectoine
pH 7.5, 32°C
Alkalilimnicola ehrlichii
0.17
-
ectoine
pH 7.5, 32°C
Paenibacillus lautus
0.34
-
ectoine
pH 7.5, 32°C
Acidiphilium cryptum
0.49
-
ectoine
pH 7.5, 32°C
Halomonas elongata
1.31
-
ectoine
pH 7.5, 32°C
Virgibacillus salexigens
1.44
-
ectoine
pH 7.5, 32°C
Pseudomonas stutzeri
Other publictions for EC 1.14.11.55
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
741719
Tao
Ectoine and 5-hydroxyectoine ...
Virgibacillus halodenitrificans, Virgibacillus halodenitrificans PDB-F2
Appl. Microbiol. Biotechnol.
100
6779-6789
2016
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
742383
Widderich
Strangers in the archaeal wor ...
Nitrosopumilus maritimus
Environ. Microbiol.
18
1227-1248
2016
-
-
1
-
-
-
-
-
-
-
1
1
-
7
-
-
1
-
-
-
-
-
1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
741466
Yang
Crystallization and X-ray diff ...
Bacillus pseudofirmus, Bacillus pseudofirmus OF4
Wei Sheng Wu Xue Bao
55
1468-1474
2015
-
-
1
1
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
739813
Hoeppner
Overexpression, crystallizatio ...
Sphingopyxis alaskensis
Acta Crystallogr. Sect. F
70
493-496
2014
-
-
1
1
-
-
-
-
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
740729
Hoeppner
Crystal structure of the ectoi ...
Sphingopyxis alaskensis, Sphingopyxis alaskensis DSM 13593
J. Biol. Chem.
289
29570-29583
2014
-
-
-
1
7
-
-
2
-
-
2
-
-
5
-
-
-
-
-
-
-
-
2
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
7
-
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
740868
Widderich
Molecular dynamics simulations ...
Virgibacillus salexigens
J. Mol. Biol.
426
586-600
2014
-
-
-
1
36
-
-
6
-
-
-
-
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
36
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
741304
Widderich
Biochemical properties of ecto ...
Acidiphilium cryptum, Alkalilimnicola ehrlichii, Halomonas elongata, Halomonas elongata DSM 2581, Paenibacillus lautus, Pseudomonas stutzeri, Pseudomonas stutzeri A1501, Sphingopyxis alaskensis, Sphingopyxis alaskensis DSM 13593, Virgibacillus salexigens
PLoS ONE
9
e93809
2014
-
-
-
1
-
-
7
14
-
21
7
20
-
17
-
-
-
-
-
-
-
-
20
7
8
7
7
-
7
7
7
-
-
-
7
-
-
-
-
-
1
-
-
-
7
-
14
-
21
7
20
-
-
-
-
-
-
-
-
20
7
7
7
-
7
7
7
-
7
-
-
-
-
7
7
743087
Widderich
Molecular dynamics simulation ...
Virgibacillus salexigens
J. Mol. Biol.
426
586-600
2014
-
-
1
-
36
-
-
14
-
1
-
1
-
3
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
36
-
-
-
-
14
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
740784
Eilert
Synthesis and release of the b ...
Halomonas elongata, Halomonas elongata DSM 2581
J. Biotechnol.
167
85-93
2013
-
1
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
741265
Reuter
Synthesis of 5-hydroxyectoine ...
Virgibacillus salexigens
PLoS ONE
5
e10647
2010
-
-
1
1
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
739899
Bursy
Synthesis and uptake of the co ...
Streptomyces coelicolor, Streptomyces coelicolor A3(2)
Appl. Environ. Microbiol.
74
7286-7296
2008
-
-
-
-
-
-
1
2
-
1
1
-
-
43
-
-
1
-
-
-
1
-
4
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
2
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